FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005284535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47571181
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTT19191994.03437324795447No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2740310.5760441389924711No Hit
ATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGT2444190.51379636759491No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA2256110.47425982550233514No Hit
CCTAGACGCACTGTAAGTTGTCAGGGTCAATTTAGACACCACTCCCCTTA1951560.41023997281042907No Hit
CGTATATAAATTAGCCTAGTAACTTCCTTCCTGCCCCGACCTAGACGCAC1787100.37566862172288723No Hit
AGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTC1575940.33128040272954334No Hit
TCAAAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGAT1563680.3287032121401401No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCG1436520.3019727426989883No Hit
GATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCC1401340.29457750901748686No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACC1385950.29134235704596023No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATAACACAGCA1351350.28406904592089066No Hit
CCCCACCTCCCTTCACCTCCCTATGATTCGCCCTTTCCCGTCCACAGAGA1289720.2711137232434906No Hit
CCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTTAATTCCCCTATGGTCCTACTTTACAGTCTGAAT1247440.26222598930222063No Hit
ATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG1149120.24155801387398812No Hit
GTGTGATGTGAGCTTGCATAGTACAGAGCATGCAGCCCTTGTGACGATGACACGTTTGAGAGTGCTCCCGGGAC1105100.23230451226342266No Hit
ATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCA1073330.22562609912921858No Hit
CACCACTCCCCTTATACCCCACCTCCCTTCACCTCCCTATGATTCGCCCTTTCCCGTCCACAGAGACACGGCTTC1057330.2222627182621344No Hit
CGAAGGTACCCTGCCTCCCGACGTACTTGTGATTCATCAATCAAAATACA970150.20393649676260928No Hit
GTTTCTCTCACCCTTGCATCCTCATTACTCTCTTAGACGCTTGAATCATG962820.2023956479028763No Hit
GGATAGTCGCGCCGTAGAAAACTCTGCCAGGGCAGAGGGATTCGCCTATA941960.19801064009741529No Hit
GGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAG931530.19581813619468477No Hit
TGAAAATCATCACCACCATCCTTAGGAATTTATTCAAGAGAAGGTAAAGA929310.19535146709937684No Hit
CAGTGTTCATGGACTCCTGATGTGTCGGGGTCGTCGAAGAGAGGTTTAGC923370.1941028119524718No Hit
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG901080.18941720198201512No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT881410.18528234562854348No Hit
GTTAGTTCAGAGGTGCTTAGCAGATTGTCTCAGCTCAGGAATCGTCGTAT838800.1763252419568898No Hit
AAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCC815350.17139578687356952No Hit
AGAACACTTGCACACATGTGCTAATGTTTCTCTCACCCTTGCATCCTCATTACTCTCTTAGACGCTTGAATCATG796820.1675005714068776No Hit
AGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTG786620.16535641610411145No Hit
AGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAA781550.16429064479185412No Hit
CATTGGTCTGTTTTAGGTAGTATCTTCCATTCAGTATTTTATTCGGAAGATGTTAAAAGGAGCAGAAATAGCGAT778950.16374409540095294No Hit
GTTATGTTATGTGTGTTACTTTTATGAAGTTAAAATTAGCTTTATATCAGAATTTCTTATTTTTTAGTTAAGTGT747700.15717499214492908No Hit
GCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGG745470.1567062209365792No Hit
AAAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTTA717160.1507551389148821No Hit
AGAACGAGAGAAGGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCACTGCCGCGTCTGACGGGACAAGGG708560.14894732169882435No Hit
AGGTCGTGGATGAACCTATTCCTTGGGGATGTTCGGTAGCTGGACGGAGG699390.1470196840393767No Hit
CTTCCTTCCTGCCCCGACCTAGACGCACTGTAAGTTGTCAGGGTCAATTT696860.146487849439769No Hit
GAATATTAATGTTTCCCGGTTTTAATGTGTGATTTTTTGAGCTGTGGTCT689070.14485030338010738No Hit
TTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA665130.13981784475773262No Hit
ACTTCCTTCCTGCCCCGACCTAGACGCACTGTAAGTTGTCAGGGTCAATT664630.13971273910563625No Hit
CCTCCCTTCCTTAATTCCCCTATGGTCCTACTTTACAGTCTGAATAAATTTTACCATATCGGATCATCGAATAGA658630.13845147128047966No Hit
GAACGAGAGAAGGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCA649480.1365280378471159No Hit
GTAAAGAACACTTGCACACATGTGCTAATGTTTCTCTCACCCTTGCATCC644580.13549800245657134No Hit
CGTCAAGCACCGAAATAATATCGTTAGCGCCTCGATCCTGGAGAATGACGATACGTTTTTACGCTCAACGCGACC644460.13547277710006822No Hit
AGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTG638590.1342388367444567No Hit
GGAGAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAA633680.13320669924087022No Hit
ATTTAGACACCACTCCCCTTATACCCCACCTCCCTTCACCTCCCTATGAT626190.13163221657246643No Hit
CGTGTGCACACAAATGGACGCGTTGCGATGGGGAGAGTGTGTGGGCTGGACCAACGGAGGTGTTTAAGATACACA613160.1288931632788347No Hit
TGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTG606880.12757303628850417No Hit
CTATTGTATGTTAGTTCAGAGGTGCTTAGCAGATTGTCTCAGCTCAGGAATCGTCGTATATTTGATAAATCAATA604830.127142103114909No Hit
AGGAGAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGA601610.1264652227154083No Hit
CGTATATAAATTAGCCTAGTAACTGCCTTCCTGCCCCGACCTAGACGCAC598590.12583038457674617No Hit
GTGTAGGGGAATCAGCTTAATGTGTCTGTCCATGTCTGAATTTATTGCAGAAATTGAAAAGAAGGGGAAGGGGA592510.12455229984725416No Hit
AGGGAATTTACCTACAGAGGACTCGGGATCCCTGGGCCACTGTCATACGA592210.12448923645599633No Hit
GTCTGAATAAATTTTACCATATCGGATCATCGAATAGAAGCCGTGACGTA579910.12190363741442535No Hit
TAATGTTTCCCGGTTTTAATGTGTGATTTTTTGAGCTGTGGTCTGTACACTTTATTGTTGTCCATCGTCTGTATA579820.12188471839704801No Hit
GAACACTTGCACACATGTGCTAATGTTTCTCTCACCCTTGCATCCTCATTACTCTCTTAGACGCTTGAATCATGA568700.11954716869442446No Hit
CAATAACGTCTCTTTCAGAAGGCATTGGTATCTTTTCCCCACTTCCAAGC567710.11933905950327363No Hit
GCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCGCT566530.11909101016432616No Hit
AGGAGAGGCTACGTGAACAGGAGGAGAGGCTGTGTGAACAGGAGGAGAGG560110.11774145359140863No Hit
AAGGGATCAACCTTTCCCGGCAAGGCGGAGGAATTGGGGCAAGAGGTCCC557780.1172516612526395No Hit
GATTAGCCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTTAA551580.11594835116664436No Hit
ATGTGCTAATGTTTCTCTCACCCTTGCATCCTCATTACTCTCTTAGACGC550160.11564985111469064No Hit
AGGAACTGAGCGGGAAAGAGAAAATATGGGACAGCGAAAATGAAACGTAA539330.11337326269028301No Hit
ATAAAACGAATAGCTCGTAACCAAACATGCACAGCGGTCAAACAGTATGT536350.11274683300378857No Hit
GTTACAAGGTGAGAAAATGAAGAAGGTGACTCACGGATATTTACGAAAAT534650.11238947378666088No Hit
AGTAAGCATAAGCCACATCATTATTGCTTCATTTCCCAATTAACATACTC530770.11157385392639295No Hit
GGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAA524040.11015913184917567No Hit
TGTAAGTTGTCAGGGTCAATTTAGACACCACTCCCCTTATACCCCACCTC520350.10938345213670436No Hit
ATTCATAAAGCCCCAGCCCCTGATCAGCGTCGGCGCAAATCAAATAATTT516140.10849846254605282No Hit
CATGAATCATACCGATTAATTCTGTGCTACTCTTCGCTTCCTCGAACACC514410.10813479698979936No Hit
GAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTT505070.10617142340863894No Hit
GAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTA503840.10591286350448186No Hit
TCGCCCTTTCCCGTCCACAGAGACACGGCTTCAGCATATATTCGCGAACA499900.10508463096596235No Hit
CACAGAGACACGGCTTCAGCATATATTCGCGAACATTCCCAAGTTGGCAA494810.1040146554276212No Hit
ATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGTTTCCT494010.10384648638426697No Hit
GGGCAAGCTAAGCTCGATCGCCCAAAGCGGCGGCTAACCATAACTACGGATCCCCGCAGGCCACTTGTAGTTCCC493170.10366990888874507No Hit
GTTAAAATTAGCTTTATATCAGAATTTCTTATTTTTTAGTTAAGTGTATT491620.10334408136724628No Hit
GGAAAGGAACGCCCAAGAAGGGGGGCACGGAGAAAAATGGGAGGAGCAGAAGTGAAAGAGAAAGAGAAGATGGTG490080.10302035595878942No Hit
GGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGA488510.10269032421120679No Hit
AGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAG483420.10162034867286561No Hit
TGTACACTTTATTGTTGTCCATCGTCTGTATAATTAAAGGGTGCTGTGTC483280.10159091909027863No Hit
GGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGCTTGAAGCTGATCTC483160.1015656937337755No Hit
CAAATAATTTTTTTTTTTGATTTGTTCGACTGCATAGCCGGTGTTGGATT482370.1013996268034632No Hit
GGGATGCTACCCGGAGCACCAGTATGCGGCCGAGCGCGCTGGAGCAGGCC478630.10061343652578228No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)