FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005284548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56769113
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGC3737970.6584513659743108No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC3108050.547489618166132No Hit
CTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACT2765090.48707648470744996No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG2597070.45747940433735507No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGC2582490.4549111063264279No Hit
GTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGG2294150.4041194020417405No Hit
GGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTC1650190.29068447837118755No Hit
CGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATT1632150.28750669400101425No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAA1526060.2688187148529166No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGT1455980.25647397379627895No Hit
GTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGT1142730.20129432002927364No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTC1095150.19291300182900517No Hit
GTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCC1083950.19094009800716807No Hit
CTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGG1071240.18870120447363692No Hit
GGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCT989790.1743536137335808No Hit
CACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACA982560.1730800338557342No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGC964630.1699216262195254No Hit
GTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGT912220.16068949324608964No Hit
CCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA882490.15545249051187396No Hit
CTCCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA813070.14322400985902317No Hit
CTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATG784850.13825299683650158No Hit
CTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGG727830.12820880255782752No Hit
GGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGAC679510.11969713178361621No Hit
GTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATT615940.10849914107342137No Hit
CGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCG592880.10443707302596043No Hit
GGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTT588260.10362325019945265No Hit
GGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGGCTTCACGCTCAGG575950.10145481751670138No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)