FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005284550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3593400
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA109507830.474703623309402No Hit
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA1438804.004007346802471RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA1156713.2189848054767074No Hit
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG722922.0117994100294982RNA PCR Primer, Index 1 (100% over 29bp)
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA679791.8917738075360384RNA PCR Primer, Index 1 (100% over 23bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG679751.8916624923470806Illumina Small RNA Adapter 2 (100% over 21bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC666881.8558468302999942No Hit
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC533261.4839984415873546RNA PCR Primer, Index 1 (100% over 27bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG377721.0511493293259864No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGAATTCTCGGGTGCC352460.9808537874993042No Hit
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGC324120.901986976122892No Hit
CGCGACCTCAGATCAGACGTGGCGACCTGGAATTCTCGGGTGCCAAGGAA227400.6328268492235766RNA PCR Primer, Index 1 (100% over 23bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG220630.6139867534925141RNA PCR Primer, Index 6 (100% over 34bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACATGGAATTCTCGGGTGCCA216210.6016864251127066No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA203590.566566482996605No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTTGGAATTCTCGGGT201890.5618355874659097No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC191160.5319752880280514No Hit
GGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCAAGGA182030.5065675961484944RNA PCR Primer, Index 1 (100% over 22bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCC171700.47782044860021144No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTTGGAATTCTCGGGTGCCAAGGA165890.4616519174041298RNA PCR Primer, Index 1 (100% over 22bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA156500.4355206767963488RNA PCR Primer, Index 1 (100% over 22bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC154000.4285634774865031RNA PCR Primer, Index 1 (100% over 27bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC134270.37365726053319975RNA PCR Primer, Index 1 (100% over 31bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA127500.35481716480213726RNA PCR Primer, Index 1 (100% over 28bp)
CCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAAC101380.28212834641286805RNA PCR Primer, Index 1 (100% over 24bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA98400.2738353648355318RNA PCR Primer, Index 1 (100% over 23bp)
GTCTACGGCCATACCACCCTGAACGTGGAATTCTCGGGTGCCAAGGAACT96370.26818611899593703RNA PCR Primer, Index 1 (100% over 25bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA81110.22571937440863804No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGTGGAATTCTCGGGTGCCAAGG80420.2237991873991206Illumina Small RNA Adapter 2 (100% over 21bp)
CTCCCACTGCTTCACTTGACTAGCCTTGGAATTCTCGGGTGCCAAGGAAC78260.21778816719541383RNA PCR Primer, Index 1 (100% over 24bp)
CGCGACCTCAGATCAGACGTGGCGACCCTGGAATTCTCGGGTGCCAAGGA69980.19474592308120442RNA PCR Primer, Index 1 (100% over 22bp)
AGAGGTGTAGAATAAGTGGGAGGCCCTGGAATTCTCGGGTGCCAAGGAAC69610.19371625758334726RNA PCR Primer, Index 1 (100% over 24bp)
CGACTCTTAGCGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCAAGGAA69400.1931318528413202RNA PCR Primer, Index 1 (100% over 23bp)
CCCCCCACTGCTAAATTTGACTGGCTATGGAATTCTCGGGTGCCAAGGAA68340.19018200033394556RNA PCR Primer, Index 1 (100% over 23bp)
TCCTGTACTGAGCTGCCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT65490.18225079312072132RNA PCR Primer, Index 1 (100% over 30bp)
CTCCCACTGCTTCACTTGACTAGCCTTTGGAATTCTCGGGTGCCAAGGAA65310.1817498747704124RNA PCR Primer, Index 1 (100% over 23bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC63900.1778260143596594No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATTCTCGGGTGCCAA63430.17651806088940836No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA62670.17440307229921523RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC56100.1561195525129404No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG55210.15364278955863528No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC53770.14963544275616408No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTGGAATTCTCGGGTGC53340.1484388044748706No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA52510.14612901430400177No Hit
GGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTG49830.13867089664384705No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTATGGAATTCTCGGG47210.13137975176712863No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATGGAATTCTCGGGTGC46670.12987699671620193No Hit
GGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAAGGAA44430.12364334613458007RNA PCR Primer, Index 1 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC44370.12347637335114377No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA44090.12269716702844104No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTTGGAATTCTCGGGTG44050.12258585183948349No Hit
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA43460.1209439528023599RNA PCR Primer, Index 1 (100% over 32bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC43240.12033171926309344RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT43000.11966382812934825No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTATCGGGTGCCA40040.11142650414649079No Hit
AGGTTCCGGATAAGTAAGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCA39480.10986809150108533RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT38720.1077531029108922RNA PCR Primer, Index 1 (100% over 30bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC38470.1070573829799076RNA PCR Primer, Index 1 (100% over 24bp)
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG37970.10566594311793845RNA PCR Primer, Index 1 (100% over 29bp)
CCCCCCACTGCTAAATTTGACTGGCTTGGAATTCTCGGGTGCCAAGGAAC37490.10433016085044805RNA PCR Primer, Index 1 (100% over 24bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA37250.10366226971670284No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC37250.10366226971670284RNA PCR Primer, Index 1 (100% over 26bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC36990.10293872098847887RNA PCR Primer, Index 1 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGCA455.456968E-1269.9983826
TTATAAT252.8428494E-669.9974122
GCTAGTC252.8428494E-669.997412
TTCGCAT550.069.99740627
CCGTTAC4050.069.9935154
TGAGTTA1850.069.98962418
GTGTTAT401.364242E-1069.9896219
TCCTACA850.069.98679
TGCCCCT353.7234713E-969.98572514
ATGGTAC207.938923E-569.98572511
GGCTGGT1385000.069.9644241
GTCTACG199850.069.957541
TACGGCC199500.069.888264
CTACGGC199850.069.8874743
GCTGGTC1388150.069.8486562
CTGGTCC1387650.069.843743
GTTTCCG94700.069.842751
TGGTCCG1398200.069.835824
TCTACGG200150.069.805062
ACGGCCA199800.069.765815
Produced by FastQC (version 0.11.5)