FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005284551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30185517
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACT3859541.2786065582378463No Hit
GTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCC2783410.9221011520193608No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGT1976670.6548405316364135No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGC1729780.5730496515928483No Hit
GTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGT1495390.4953998303226014No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTCTAATACTGGTG1490570.49380303805960984No Hit
CTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTG1420170.4704805950482809No Hit
CCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA1258350.41687210459241103No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTC1189330.39400683446965645No Hit
GTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATT1170630.3878118105447722No Hit
CTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATG1152520.38181224459398855No Hit
GGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGAC1140910.3779660292053305No Hit
CTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTA1119670.37092954213770796No Hit
GTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGT1033900.3425152532587068No Hit
GGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTG988990.3276372572979287No Hit
CTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGG949630.3145978914325039No Hit
GTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTT930080.308121275511034No Hit
GGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCT892370.2956285294036872No Hit
GTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCA866050.2869091160505881No Hit
CTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGG777770.2576633025699046No Hit
GTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTC775450.25689472206157676No Hit
AGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGAC747440.24761543756232499No Hit
GCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG724100.239883252620785No Hit
CCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATA684850.2268803280725654No Hit
AGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTT649300.21510315692124804No Hit
GCAGGTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATT616590.2042668343232286No Hit
CTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG599850.19872112841400066No Hit
GGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTT588250.19487822587236125No Hit
GTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGACTGGTGAAG558060.18487674072304278No Hit
GGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTC539800.17882748206697935No Hit
CTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTTTCTCTTGCGTCT539360.17868171679815853No Hit
GGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTT532120.17628321555665255No Hit
CTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA527130.17463010489434388No Hit
GTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATA502330.16641424428808027No Hit
GTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTA486280.1610971248231395No Hit
GGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATT442830.14670280452708495No Hit
CCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTTT435610.14431092897961628No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAG424870.14075293128158117No Hit
CACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACA423150.14018312159437257No Hit
CTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGG413540.13699947560944542No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTAT412180.13654892841490837No Hit
GGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCCCTAATT411850.13643960446329276No Hit
CGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGG390200.1292672906679054No Hit
CCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCAC377650.1251096676594938No Hit
ATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG373510.12373814899377074No Hit
CGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCA372330.1233472330455695No Hit
CCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAG368380.1220386584732009No Hit
AGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCC364950.12090235194580234No Hit
CGTTAAACATGTGTCACTGGGCAGGCGGTGCCTCTAATACTGGTGATGCT362780.1201834641427543No Hit
GATCAATTGAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTA360780.11952089473902335No Hit
GTCACTGGGCAGGCGGTGCCTCTAATACTGGTGATGCTAGAGGTGATGTT355700.11783796845354678No Hit
CTCGTGGAGCCATTCATACAGGTCCCTAATTAAGGAACAAGTGATTATGC351300.11638031576533872No Hit
CCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCAT343400.11376316662060153No Hit
GTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG341880.11325961387376603No Hit
GGCAATGCTTGTGGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTA323410.10714078543031084No Hit
GGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGACTGGTGAA321840.10662066844838204No Hit
CGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTGCTCGG312660.10357947488525705No Hit
GCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGT312250.10344364815749223No Hit
GCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGT311470.10318524609003715No Hit
GTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTG308310.10213838643214226No Hit
GTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGA306690.10160170521512021No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCGA2850.050.0535746
TTAAGCG11900.038.27754228
TTACGCG3100.037.1967866
CGCGAAA6600.037.0017664
CCGCTAG4000.034.7799032
TTCGCGA10300.034.45553646
GACGTTA39250.033.39768212
TTATGCT856700.033.34970570
ACGCGTA7200.032.8380520
CGCGAAT10600.032.82792748
GTTCGCG11150.031.82703245
CGATTAA8000.031.7371835
CGTTAAC41900.031.45139114
CTAGTCG4550.031.340655
TCGACGG6450.031.27132232
CGCGTAT4500.030.9154662
ACGTTAA42400.030.83447813
CGGATCG6050.030.4784431
CGCGACA1950.030.31300215
CGCTATA3800.030.29048363
Produced by FastQC (version 0.11.5)