FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005284557

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284557
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57432425
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACT9676341.6848217709769349No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGT7246201.261691457395365No Hit
GTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGT4599040.8007741271590046No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGC4055030.7060523737244249No Hit
GTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGT3371530.587042946558499No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTC3354410.5840620520550891No Hit
CACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACA3266730.5687954147852889No Hit
GGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGAC3264320.568375791201573No Hit
GTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCC3224350.5614163079479928No Hit
GGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCT3121140.5434456232694336No Hit
GTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGG2853250.4968012407625135No Hit
GGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTC2780260.4840923920590154No Hit
CTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGG2706080.4711763433287033No Hit
CTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA2627380.4574732827318366No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG2622210.4565730943800475No Hit
CGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATT2254770.39259529786527386No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTCTAATACTGGTG2179800.3795416961759842No Hit
CCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA2131480.3711283303813134No Hit
GTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATT2093140.3644526589291676No Hit
CTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGG2080390.3622326586418735No Hit
GGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTT2060900.35883910526153123No Hit
GGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTG2044610.35600272842388253No Hit
CTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTA1869410.32549731271141696No Hit
CTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATG1860020.3238623477939509No Hit
GGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTC1629780.28377349554715825No Hit
AGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGAC1606460.27971307149227986No Hit
CTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTG1585170.27600610630667255No Hit
GCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGT1540610.26824742294966647No Hit
GTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTT1252010.21799706350550233No Hit
CGGTGAACCGTCAAAGCCAGGCTATCTAAATGGTTTTAAGCGTGGCCCGT1249040.21747993402681498No Hit
GTCTAAATTGACCCTGACAACTTACAGTGCGTCTAGGTCGGGGCAGGAAG1238620.21566562790966948No Hit
GGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATT1202050.20929814473270109No Hit
CCCTCGTGGAGCCATTCATACAGGTCCCTAATTAAGGAACAAGTGATTAT1189210.20706247385514367No Hit
GCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG1166330.2030786615748856No Hit
GTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTC1104160.19225376605636973No Hit
CCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAG1069120.18615268291387663No Hit
GTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCA1027490.1789041643287742No Hit
CGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGG1016430.17697842290309004No Hit
TTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGA1010510.17594764629910717No Hit
GTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATA987040.17186110459379No Hit
ATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG980400.17070496326770113No Hit
GTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG939750.16362707999879858No Hit
CATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG920370.16025267956211148No Hit
GGCTAATTTATATACGTCACGGCTTCTATTCGATGATCCGATATGGTAAA913880.15912265588646135No Hit
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGAACTTCCATAGGGCTGGCAGGAGTC892970.15548185541529894No Hit
CAGCACTACAGAAAAAATTCAAACAGGTCCCCGAGGCGTTTTGAAATTCATCCCAACTGTAGGCTGAGTGACCTG883710.1538695257948798No Hit
CCTCGTGGAGCCATTCATACAGGTCCCTAATTAAGGAACAAGTGATTATG882390.15363969047101877No Hit
GCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATG877540.15279521977349902No Hit
AGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCC855170.14890020750473273No Hit
AGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTT852630.14845794862396983No Hit
CTCTCCTTTCTCTCTCCTCCTCCTGCAGCTTGATGGAGATACCTCTTACTGGGCCTCTCTGAATTCGCTTCATC840830.1464033601227878No Hit
GTCTAGGTCGGGGCAGGAAGGAAGTTACTAGGCTAATTTATATACGTCAC811610.14131564181731837No Hit
CTCGTGGAGCCATTCATACAGGTCCCTAATTAAGGAACAAGTGATTATGC779100.13565507637889224No Hit
CCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCAC776930.13527724103587824No Hit
GTGTCTCTGTGGACGGGAAAGGGCGAATCATAGGGAGGTGAAGGGAGGTG773080.1346068880079502No Hit
ACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCCCTAATTAAGGAACAAGTGATT763350.13291272308282298No Hit
CTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGA758350.13204213473486448No Hit
CTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG738260.12854411075276728No Hit
GTGCGTCTAGGTCGGGGCAGGAAGGAAGTTACTAGGCTAATTTATATACG723770.12602114572038356No Hit
GTATGGTTTAGGGCCCTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGGGAATGTTCGCG719690.12531074562844943No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA719300.12524283973730868No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC706210.12296363944235335No Hit
CCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTTT680640.1185114506308936No Hit
GTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGTTTCGTTTCCCCGGGACCTCTTGCCCC678410.11812316822770413No Hit
GGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGAC672910.11716552104494979No Hit
GGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAAT669610.11659093273529716No Hit
GGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCG662130.11528853256675127No Hit
GGCTTTCTCTTTCCTCTTCTCCTCCAGGGTGGCTGTCACTGCCTGGTACT661750.11522236785230643No Hit
GTCACTGGGCAGGCGGTGCCTCTAATACTGGTGATGCTAGAGGTGATGTT654240.11391474415367278No Hit
CCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATA649770.11313643817059789No Hit
GCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAAT647610.1127603440042798No Hit
GTCCAACTTAATGAAACCGATATCCTTCGCGTACTGACGGAAACACTGGC636700.1108607202290344No Hit
GCCTACAGCACCCGGTATTCCCAGGCGGTCTCCCATCCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCG631980.11003888482856157No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC620840.10809921398931004No Hit
GTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCG604200.10520189596730418No Hit
AGCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGG593990.10342415456077293No Hit
GGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTT590600.1028338956608571No Hit
GTTCCTTTTACTTTTTTTAACCTTTCCTTATGAGCATGCCTGTGTTGGGT575720.10024302473733261No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)