FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005284569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1687743
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTTACTCGTCTATCGGCTAGGATGCGCGCCTTGGAATTCTCGGGTGCC1124246.6612037496230165No Hit
TCTTACTCGTCTATCGGCTAGGATGCGCGCCTTGGAATTCTCGGGTGCCA730194.326428846098014No Hit
CTTACTCGTCTATCGGCTAGGATGCGCGCCTTGGAATTCTCGGGTGCCAA388942.304497781949029No Hit
GCTACGATCTGCTGAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC371452.200868260155723RNA PCR Primer, Index 1 (100% over 31bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA281901.6702779984867366RNA PCR Primer, Index 1 (100% over 34bp)
CGGTGTGTAGGGGAACCAGGAATGGAATTCTCGGGTGCCAAGGAACTCCA198881.1783784616496706RNA PCR Primer, Index 1 (100% over 28bp)
GCCTTGGCTGGAAGAATTTGGACCTGGCTTGGAATTCTCGGGTGCCAAGG186031.1022412772560752Illumina Small RNA Adapter 2 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA172661.023023055050443No Hit
CCTCCTGGTGAGCCTGCATGTCGTTGGAATTCTCGGGTGCCAAGGAACTC151460.8974115134827992RNA PCR Primer, Index 1 (100% over 26bp)
TCTTTGCTCGTCTATCGGCTAGGATACTCGCCTTGGAATTCTCGGGTGCC151100.8952784873052354No Hit
CGGTATGTACTATCCGGCTGAGCCTTGGAATTCTCGGGTGCCAAGGAACT150360.8908939334957988RNA PCR Primer, Index 1 (100% over 25bp)
ACTATCCGGCTGAGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC143400.8496554273962327RNA PCR Primer, Index 1 (100% over 33bp)
CGGGCTTGGCAGAATCAGCGGGGATGGAATTCTCGGGTGCCAAGGAACTC117860.6983290702435145RNA PCR Primer, Index 1 (100% over 26bp)
AGGCTGGAGGAACGTAGTACCCTTGGAATTCTCGGGTGCCAAGGAACTCC110870.6569128119624847RNA PCR Primer, Index 1 (100% over 27bp)
GGCTTAGTGAGATCTCCGGATTGGCTTGGAATTCTCGGGTGCCAAGGAAC104360.6183405885848734RNA PCR Primer, Index 1 (100% over 24bp)
CCTCCATCGTTTTTTTACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC97390.5770428317581527RNA PCR Primer, Index 1 (100% over 31bp)
GCCTTGGCTGGAAGAATTTGGACCTGGCTGGAATTCTCGGGTGCCAAGGA96750.5732507852202616RNA PCR Primer, Index 1 (100% over 22bp)
CCTGGTGAGCCTGCATGTCGTTGGAATTCTCGGGTGCCAAGGAACTCCAG92300.5468842116364874RNA PCR Primer, Index 1 (100% over 29bp)
GGAGCACCTTCGACCGATCTGGATCTTGGAATTCTCGGGTGCCAAGGAAC89490.530234757306059RNA PCR Primer, Index 1 (100% over 24bp)
CTTGTGAAACTCAGTCGTGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG84000.49770610809821164RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA83810.4965803442822752RNA PCR Primer, Index 1 (100% over 28bp)
CCTTGGCTGGAAGAATTTGGACCTGGCTTGGAATTCTCGGGTGCCAAGGA81260.48147140885786516RNA PCR Primer, Index 1 (100% over 22bp)
TACTCGTCTATCGGCTAGGATGCGCGCCTTGGAATTCTCGGGTGCCAAGG73130.4333005676812169Illumina Small RNA Adapter 2 (100% over 21bp)
GCCTTGGCTGGAAGAATTTGGACCTGGCTGGAATGGAATTCTCGGGTGCC73090.43306356477259866No Hit
CGAGCTGTAGTGTAGTTGGTTATCACTTTTGGTTTGGAATTCTCGGGTGC72760.43110829077649854No Hit
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG67570.4003571633832876RNA PCR Primer, Index 1 (100% over 29bp)
GTCCCACTCCGTAGATCTGTTCTGGAATTCTCGGGTGCCAAGGAACTCCA61300.3632069574573854RNA PCR Primer, Index 1 (100% over 28bp)
TCTTCACTCGTCTATCGGCTAGGATATGTGCCTTGGAATTCTCGGGTGCC60130.35627462238030316No Hit
AGGTTCCGGATAAGTAAGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCA59840.35455635129282126RNA PCR Primer, Index 1 (100% over 28bp)
GGAGCACCTTCGACCGATCTGGATTGGAATTCTCGGGTGCCAAGGAACTC55490.3287822849805924RNA PCR Primer, Index 1 (100% over 26bp)
GCCTTGGCTGGAAGAATTTGGACCTGGCTGTGGAATTCTCGGGTGCCAAG54200.32113894117765557No Hit
CGGCAGGTCGTGGGTTCGATCCCCACAATGGGCTCTGGAATTCTCGGGTG51940.30774827684072753No Hit
GCCTTGGCTGGAAGAATTTGGACCTGGCTGGATGGAATTCTCGGGTGCCA51220.3034822244856No Hit
TCTTAACCCGGACCAGAAACTATGGAATTCTCGGGTGCCAAGGAACTCCA50990.3021194577610454RNA PCR Primer, Index 1 (100% over 28bp)
GTTGGCTTAGTAGTTTGGACGTGGAATTCTCGGGTGCCAAGGAACTCCAG49390.29263934141631753RNA PCR Primer, Index 1 (100% over 29bp)
TCGGCAGGTCGTGGGTTCGATCCCCACAATGGGCTCTGGAATTCTCGGGT48360.286536516519399No Hit
CGGTATGTACTATCCGGCTGAGCCTTTGGAATTCTCGGGTGCCAAGGAAC48130.2851737497948444RNA PCR Primer, Index 1 (100% over 24bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC46590.27604913781304385RNA PCR Primer, Index 5 (100% over 35bp)
GGCTGGAGGAACGTAGTACCCTTGGAATTCTCGGGTGCCAAGGAACTCCA44560.26402124020067036RNA PCR Primer, Index 1 (100% over 28bp)
TCTTTACTCGTCTATCGGCTAAGATATCCGCCTTGGAATTCTCGGGTGCC44190.26182896329595207No Hit
TCTTCACTCGTCTATCGGCTAAGATATGCGCCTTGGAATTCTCGGGTGCC43600.2583331703938337No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACA42990.25471887603740617RNA PCR Primer, Index 5 (100% over 36bp)
CCATCGGCTAGCACTCGGTTCGATTCCGGGCTATTGTTGGAATTCTCGGG42140.2496825642292695No Hit
TCGGCTAGCACTCGGTTCGATTCCGGGCTTGGAATTCTCGGGTGCCAAGG41830.24784579168747847Illumina Small RNA Adapter 2 (100% over 21bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC41140.2437574915138146No Hit
GTCGCCTTAGTTTAGTGGTAGAATATCTGACTTGGAATTCTCGGGTGCCA41090.24346123787804186No Hit
AAAGGCTGGAGGAACGTAGTACCCTTGGAATTCTCGGGTGCCAAGGAACT39860.23617339843803234RNA PCR Primer, Index 1 (100% over 25bp)
CCGCAGCAGGTCTCCAAGGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG39290.23279610699022302RNA PCR Primer, Index 1 (100% over 29bp)
GGTACGGACAAGGGGAATCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT39030.23125558808420477RNA PCR Primer, Index 1 (100% over 30bp)
TCTGGCCGGGACTGAGTTTGGAAGGCTTAGGCTTGGAATTCTCGGGTGCC38610.2287670575437137No Hit
CGGTGTGTAGGGGAACCAGGAATTGGAATTCTCGGGTGCCAAGGAACTCC37310.22106446301362234RNA PCR Primer, Index 1 (100% over 27bp)
ACGGGCTTGGCAGAATCAGCGGGGATGGAATTCTCGGGTGCCAAGGAACT35620.21105109012450354RNA PCR Primer, Index 1 (100% over 25bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATGC35540.21057708430726715RNA PCR Primer, Index 5 (100% over 50bp)
CTTTGCTCGTCTATCGGCTAGGATACTCGCCTTGGAATTCTCGGGTGCCA35220.2086810610383216No Hit
TCTTTACTCGTCTATCGGCTAGGATACCCGCCTTGGAATTCTCGGGTGCC33590.1990231925121301No Hit
AAGGCTGGAGGAACGTAGTACCCTTGGAATTCTCGGGTGCCAAGGAACTC30160.1787001930981198RNA PCR Primer, Index 1 (100% over 26bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA28810.1707013449322557RNA PCR Primer, Index 1 (100% over 32bp)
CTTTCAACAACGGATCTCTTGGCTCTTGGAATTCTCGGGTGCCAAGGAAC28200.16708705057582818RNA PCR Primer, Index 1 (100% over 24bp)
GACGGGCAAAGTGCAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT28100.1664945433042827RNA PCR Primer, Index 1 (100% over 30bp)
GTCCCCTTAGTTTAGTGGTAGAATATCTGACTTGGAATTCTCGGGTGCCA28100.1664945433042827No Hit
AACTTTCAACAACGGATCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC27330.16193223731338244RNA PCR Primer, Index 1 (100% over 31bp)
GGCTTAGTGAGATCTCCGGATTGGCTTTGGGAAGCTGTGGAATTCTCGGG27280.1616359836776097No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA26960.15973996040866412No Hit
GGCTTAGTGAGATCTCCGGATTGGCTTTGGGAAGCTTGGAATTCTCGGGT26180.15511840369060928No Hit
GAACGGGGTGCTGTAAGCAGTAGAGTAGCCTTGGAATTCTCGGGTGCCAA25930.15363713551174557No Hit
CGGGCTTGGCAGAATCAGCGGGGAATGGAATTCTCGGGTGCCAAGGAACT25590.1516226107884909RNA PCR Primer, Index 1 (100% over 25bp)
GCCGGGACTGAGTTTGGAAGGCTTGGAATTCTCGGGTGCCAAGGAACTCC24770.1467640511618179RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC24190.14332750898685404RNA PCR Primer, Index 1 (100% over 27bp)
CGGGCTTGGCAGAATCAGCGGGGTGGAATTCTCGGGTGCCAAGGAACTCC23130.13704693190847186RNA PCR Primer, Index 1 (100% over 27bp)
GCCTTGGCTGGAAGAATTTGGACCTGGTGGAATTCTCGGGTGCCAAGGAA22620.13402514482358985RNA PCR Primer, Index 1 (100% over 23bp)
CACCGCCCGTCGCTACTACCGTGGAATTCTCGGGTGCCAAGGAACTCCAG21650.12827782428959858RNA PCR Primer, Index 1 (100% over 29bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC21640.12821857356244404RNA PCR Primer, Index 1 (100% over 33bp)
ACCTCCTGGTGAGCCTGCATGTCGTTGGAATTCTCGGGTGCCAAGGAACT21640.12821857356244404RNA PCR Primer, Index 1 (100% over 25bp)
CGGTGTGTAGGGGAACCAGGATGGAATTCTCGGGTGCCAAGGAACTCCAG21350.1265003024749621RNA PCR Primer, Index 1 (100% over 29bp)
CGCGCGAGGGCGGGGTTCGACTCCCCGGTAGGAATGGAATTCTCGGGTGC21110.12507828502325297No Hit
GGCTTAGTGAGATCTCCGGATTGGCTTTGGGATGGAATTCTCGGGTGCCA21100.1250190342960984No Hit
CGGTGTGTAGGGGAACCAGGAATTTGGAATTCTCGGGTGCCAAGGAACTC20490.12140473993967091RNA PCR Primer, Index 1 (100% over 26bp)
CTGGCCGGGACTGAGTTTGGAAGGCTTAGGCTTGGAATTCTCGGGTGCCA19800.11731643976600702No Hit
GATCTCCGGATTGGCTTTGGGATGGAATTCTCGGGTGCCAAGGAACTCCA19630.1163091774043797RNA PCR Primer, Index 1 (100% over 28bp)
GCTCTACACTCTACTCGTCAGCTGTTGGAATTCTCGGGTGCCAAGGAACT19600.11613142522291604RNA PCR Primer, Index 1 (100% over 25bp)
GTCCTTGTTACTCGCTGATGTGGAATTCTCGGGTGCCAAGGAACTCCAGT18620.11032485396177026RNA PCR Primer, Index 1 (100% over 30bp)
CTTCACTCGTCTATCGGCTAGGATATGTGCCTTGGAATTCTCGGGTGCCA18000.1066513088781882No Hit
GGTACGGACAAGGGGAATCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG17980.1065328074238791RNA PCR Primer, Index 1 (100% over 29bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA17080.10120024197996971RNA PCR Primer, Index 1 (100% over 28bp)
TTCTTCGTAGCACAGTGGTAGTTGCACCGAGCTTGGAATTCTCGGGTGCC16880.10001522743687873No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)