FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005284572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50629155
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCT5948581.1749317167153985No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGT4873580.9626034643477657No Hit
CTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACT4139250.8175625289420692No Hit
GGCTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTT4044540.7988559161218471No Hit
GTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGT3165200.6251733808316572No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGC3131890.6185941677280611No Hit
GGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGAC3101060.6125047909648107No Hit
GGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTC2790610.551186366827572No Hit
GCTCCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGT2761760.5454880690779847No Hit
GTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGG2668430.527054026479407No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTC2454670.48483329417605325No Hit
GTTTGGTAGTTTAGGACCTGTGGGCTTGTTAGGTACTGTTTGCATTAATA2381230.47032781803291007No Hit
GTGGGCTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGT2316790.457599973770054No Hit
GTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCC2308400.4559428258283197No Hit
GTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATT2251290.4446627639746308No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTCTAATACTGGTG2247050.4438253018443622No Hit
CTCCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA2203210.43516625944083015No Hit
GGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTG2039770.4028844644948153No Hit
CACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACA1997500.39453552009706655No Hit
GGTAGTTTAGGACCTGTGGGCTTGTTAGGTACTGTTTGCATTAATAAATT1977200.3905259726337522No Hit
AGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGAC1803240.35616632353433514No Hit
CGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATT1724260.34056661621154843No Hit
GCAGGTTTGGTAGTTTAGGACCTGTGGGCTTGTTAGGTACTGTTTGCATT1655520.3269894589392219No Hit
GTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTT1602770.31657056097420544No Hit
GGACCTGTGGGCTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTT1529230.30204533336572575No Hit
CCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGCTTGTTAGGTACTGTTTGCATTAATAA1491990.2946898876744042No Hit
CTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGG1254650.2478117598446982No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG1247430.24638570404740115No Hit
GCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG1207140.23842783866331563No Hit
ATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG1064180.21019114381822093No Hit
GCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTT1042900.20598803199460866No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGC1001280.19776747212154736No Hit
CTGTGGGCTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGG988960.19533409159208762No Hit
GTAGTTTAGGACCTGTGGGCTTGTTAGGTACTGTTTGCATTAATAAATTA988050.19515435325752523No Hit
AGGACCTGTGGGCTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCC949560.18755201424949713No Hit
GGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAAT906890.17912406399040237No Hit
GTCACTGGGCAGGCGGTGCCTCTAATACTGGTGATGCTAGAGGTGATGTT891470.17607838803551037No Hit
CCCTCGTGGAGCCATTCATACAGGTCCCTAATTAAGGAACAAGTGATTAT886480.17509278991521782No Hit
CTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATG881780.1741644710444012No Hit
GGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCG854480.16877232100753015No Hit
CATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG817210.16141094987660767No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGC795710.15716438482925502No Hit
CTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTTTCTCTTGCGTCT769930.1520724570654991No Hit
GTTCCTTTTACTTTTTTTAACCTTTCCTTATGAGCATGCCTGTGTTGGGT764450.1509900767650576No Hit
GCTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTC756200.14936058087479437No Hit
GCCTTGACTTTGGGGTTGCCCATGATGGCAGAGGCAGAGGACAGGTTGCC740660.14629120316149855No Hit
CTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTG735850.14534115767881176No Hit
GGTTTGGTAGTTTAGGACCTGTGGGCTTGTTAGGTACTGTTTGCATTAAT723850.14297098183842888No Hit
GCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCG712920.14081214667714678No Hit
CTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCT702200.13869478959307144No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA700680.13839456731995625No Hit
GGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTT669350.1322064332300233No Hit
GTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCA662140.1307823525792599No Hit
GCCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGG643250.12705130077719054No Hit
GCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAG642210.1268458855376907No Hit
CTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTA635630.12554623911854743No Hit
GCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTTATG609580.12040098239838291No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC609440.12037333034691176No Hit
CCCAGGGTTTCTCCTCCAGCATCTTCCACATTCACCTTGCCCCACAGGCT603310.11916256552178285No Hit
CCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGCTT583360.11522214818714632No Hit
AGGGCTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC581920.11493772708630037No Hit
TTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGA581910.11493575193976671No Hit
CCTCGTGGAGCCATTCATACAGGTCCCTAATTAAGGAACAAGTGATTATG568000.11218832311145624No Hit
AGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTT566410.1118742748126055No Hit
GGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGAC560510.11070893835775059No Hit
CGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGG550490.1087298415310309No Hit
GCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAAT539840.10662631047269108No Hit
ACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCCCTAATTAAGGAACAAGTGATT529280.10454055573315416No Hit
GACCTGTGGGCTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCAT527360.10416132759869288No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTT524520.1036003859831356No Hit
GTGTCACTGGGCAGGCGGTGCCTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTA523560.10341077191590499No Hit
CTCGTGGAGCCATTCATACAGGTCCCTAATTAAGGAACAAGTGATTATGC523190.10333769149415983No Hit
AGCTCCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGG521640.10303154378144372No Hit
CAGGTTTGGTAGTTTAGGACCTGTGGGCTTGTTAGGTACTGTTTGCATTA520320.10277082443900161No Hit
GCGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGAT518850.10248047789855469No Hit
CCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTTT516570.10203014448888195No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC516540.10202421904928098No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)