FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005284578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31007583
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGT7254422.3395631965251855No Hit
GGGCTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCT5153611.6620482802545429No Hit
CTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACT4462141.4390479902932132No Hit
GGCTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTT4368391.408813450567882No Hit
GTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGT4088251.3184678083422368No Hit
GCTCCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGT4002961.2909616334817198No Hit
GGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGAC3774891.2174086577467196No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGC3698611.192808223717405No Hit
CTCCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA2922140.9423952844051082No Hit
GTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCC2554650.8238791136993813No Hit
GTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATT2505360.8079830020933912No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTCTAATACTGGTG2436550.7857916561893907No Hit
GTTTGGTAGTTTAGGACCTGTGGGCTTGTTAGGTACTGTTTGCATTAATA2391790.7713564775429288No Hit
AGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGAC2272090.7327530172216261No Hit
CACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACA2218660.7155217483413654No Hit
GTGGGCTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGT1981310.6389759563007539No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTC1921760.6197709766672236No Hit
GGACCTGTGGGCTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTT1671750.5391423123821034No Hit
GTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGTTTCGTTTCCCCGGGACCTCTTGCCCC1563100.5041024964764264No Hit
ATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG1543510.4977846870554212No Hit
GGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTG1454120.4689562549909163No Hit
GTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTT1435090.4628190465538704No Hit
CTGTGGGCTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGG1408640.45428887507936366No Hit
GGTAGTTTAGGACCTGTGGGCTTGTTAGGTACTGTTTGCATTAATAAATT1357130.4376768095726778No Hit
GCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG1292020.41667872016983715No Hit
CCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGCTTGTTAGGTACTGTTTGCATTAATAA1248060.40250154292903123No Hit
AGGACCTGTGGGCTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCC1238650.3994668013950007No Hit
CTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGG1220060.39347149373106566No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA1219020.3931360919037127No Hit
CCCTCGTGGAGCCATTCATACAGGTCCCTAATTAAGGAACAAGTGATTAT1159810.3740407628675863No Hit
GGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAAT1022850.329870922219252No Hit
CCTCGTGGAGCCATTCATACAGGTCCCTAATTAAGGAACAAGTGATTATG988700.3188574872153047No Hit
GTCACTGGGCAGGCGGTGCCTCTAATACTGGTGATGCTAGAGGTGATGTT966240.3116140977515081No Hit
CATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG962080.3102724904420961No Hit
GCAGGTTTGGTAGTTTAGGACCTGTGGGCTTGTTAGGTACTGTTTGCATT882750.28468842605371725No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA866000.27928652162279144No Hit
ACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCCCTAATTAAGGAACAAGTGATT860130.2773934363087894No Hit
GGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGAC859130.27707093455171916No Hit
GTAGTTTAGGACCTGTGGGCTTGTTAGGTACTGTTTGCATTAATAAATTA854600.2756100015921912No Hit
CTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTG818930.2641063639174972No Hit
AGCTCCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGG812750.26211330305880337No Hit
GTCGGAAGATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTA803270.2590559864017779No Hit
CTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCT797850.25730802687845744No Hit
GCTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTC776150.25030973875003415No Hit
GCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTTATG762250.2458269643267584No Hit
CTCGTGGAGCCATTCATACAGGTCCCTAATTAAGGAACAAGTGATTATGC730780.23567783403175926No Hit
GGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTC726090.23416530079110004No Hit
GTCCAGCGTGGCAAACAGGAGGTCTTCTTCATAGCTGTCAGCACTCGTCA717560.23141436080329125No Hit
GTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCA717560.23141436080329125No Hit
GTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGG717130.23127568504775103No Hit
CGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGG704010.2270444619949901No Hit
GCCTTCTCTCGTTCTCGCCCGCTTTGCAAAAATATCTAATATCAATTGCG650770.20987446844857272No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC645050.2080297583981312No Hit
CTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTA629150.20290198046071503No Hit
GTCGTCGGAAGATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACT616890.19894810891903444No Hit
GGTTTGGTAGTTTAGGACCTGTGGGCTTGTTAGGTACTGTTTGCATTAAT612030.19738075037967323No Hit
GTCTGCGATAGGCTAGTTCATAAACGAGGGGCGATGCCCGGGCTCAGAATCCAAGGAGCAAAACTGCGTCGGTTT611770.197296899922835No Hit
GCCCTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGGGAATGTTCGCGAATATATGCTGA609490.19656159591671493No Hit
CTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATG599560.1933591534690079No Hit
CATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTTATGC589160.19000513519547782No Hit
GCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAAT564110.18192646618086936No Hit
GCCCCTCTGAGGTCGTAGCCGGGCTCACAGCTGTAGAACACTTCCTGCCC557170.17968830398680222No Hit
GTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCG547000.1764084611173983No Hit
ACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGC537520.1733511444603728No Hit
GTGCCATTCGGACCTACCGTAAGCCTATATTTCGTTTTTCTGAGACCTAT528980.1705969794549933No Hit
GTGTCACTGGGCAGGCGGTGCCTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTA523900.1689586705290767No Hit
GACCTGTGGGCTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCAT520160.16775251395763416No Hit
AGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTT507410.16364061655498915No Hit
CCAATACATAAAAGGCACTTCTGTAGGGCTGGCATGAGTCACACAGTTCAAGACAACCTGAAGGAGTTGAATAAC505560.16304398830440928No Hit
TTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGA496850.16023499800032784No Hit
CCTGTTGATACCGTCTGCGATAGGCTAGTTCATAAACGAGGGGCGATGCC494260.15939971844951606No Hit
GGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCCCTAATT490740.15826451226462895No Hit
GGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTT488160.15743245773138784No Hit
CCTGTGGGCTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAG485450.1565584779697276No Hit
CTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG483180.15582639898117825No Hit
AGCCGCTTGGCAGGACCATTTTTCTGGTCATCGGGTCCAGCGTGGCAAAC480860.1550781949047754No Hit
CCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGCTT480790.15505561978178048No Hit
GTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAG471600.15209182863430537No Hit
GGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGT466220.1503567691812677No Hit
CGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATT462150.149044187029992No Hit
GCCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGCTTGTTAGGTACTGTTTGCATTAATA459270.1481153819696298No Hit
TCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCAC455170.14679312476564202No Hit
ATCGGGTCCAGCGTGGCAAACAGGAGGTCTTCTTCATAGCTGTCAGCACT449240.14488068934621573No Hit
GTGCGGTCAATCATCTTCACTTCAATTGCTGTTGCCAATGACTTCAGCTGACTTGGCGACAGTTCATCATTAA446910.14412926025224218No Hit
GGTCAATCATCTTCACTTCAATTGCTGTTGCCAATGACTTCAGCTGACTTGGCGACAGTTCATCATTAAAGATG437190.14099454317351984No Hit
CTCTCCTTTCTCTCTCCTCCTCCTGCAGCTTGATGGAGATACCTCTTACTGGGCCTCTCTGAATTCGCTTCATC430930.1389756821742604No Hit
CCAGAATTCAAGACGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCT428140.13807590227203456No Hit
CAGGTTTGGTAGTTTAGGACCTGTGGGCTTGTTAGGTACTGTTTGCATTA422910.1363892180825574No Hit
GCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGT416390.13428650662645972No Hit
GCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAG414880.13379952897328373No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA414310.1336157029717537No Hit
CGTGGAGCGGCGGGAGGAGGCCCAGCGGGCACAGCAGGCTGTGCCTGGCCCTGGGCCCATGACCCCAGAGTCTGC405780.13086476298394492No Hit
AGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCCCTAATTAAGGAA405710.13084218786095003No Hit
GTCGGTTTAGCTGAGATTGCACCGTTGTTATAATCGACCCGGGCTGCGAC405680.1308325128082379No Hit
GCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTT403700.1301939593292389No Hit
GACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCCCTAATTAAGGAAC400530.1291716287593264No Hit
GTTTAGGACCTGTGGGCTTGTTAGGTACTGTTTGCATTAATAAATTAAAG399980.12899425279293777No Hit
GACGGCCTTAGTACCCTCGGACACGGCGTGCTTGGCCAACTCCCCAGGCA384960.12415027640174339No Hit
AGGGCTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC379150.12227654119316554No Hit
GGTTTAGCTGAGATTGCACCGTTGTTATAATCGACCCGGGCTGCGACAAT374690.12083818335663248No Hit
CGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCA372740.12020930493034558No Hit
CCCAGGGTTTCTCCTCCAGCATCTTCCACATTCACCTTGCCCCACAGGCT371900.11993840345440661No Hit
AGCTGAACCCTCGTGGAGCCATTCATACAGGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCACGGTT369220.11907409874545849No Hit
CACTGGGCAGGCGGTGCCTCTAATACTGGTGATGCTAGAGGTGATGTTTT367840.11862904632070162No Hit
GGATCGAGGTCATGACTGGACACTGCATCGGAAGACACTTCGTGCCGACACGAAGCAGCCTCAATATCTAACACG367510.11852262074086846No Hit
GTTTGTATAGCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTTTCTTCTTTCACGGTATCGGCTTCTATGGCG366920.11833234470419703No Hit
GTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCTGCGTTTGTATAGCCGACAAGCGCAATTTGAAGCAC365320.11781634189288472No Hit
TGGTAGTTTAGGACCTGTGGGCTTGTTAGGTACTGTTTGCATTAATAAAT365090.11774216648875857No Hit
CGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGC363270.1171552132908908No Hit
TGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTTAT356310.11491060106168223No Hit
GTTCCTGTTGATACCGTCTGCGATAGGCTAGTTCATAAACGAGGGGCGATGCCCGGGCTCAGAATCCAAGGAGCA349640.11275951434202403No Hit
CAGCTGAACCCTCGTGGAGCCATTCATACAGGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCACGGTT349420.11268856395546857No Hit
TGGGCTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC348290.11232413696997925No Hit
GCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGG346320.11168880850855095No Hit
AGGTTTGGTAGTTTAGGACCTGTGGGCTTGTTAGGTACTGTTTGCATTAA345950.11156948285843497No Hit
GTTTCATCAATGGCACTCTCTCACAGCCAATAACTTCAACAACTTCCCTA341580.11016015018003823No Hit
GCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCG331920.1070447832067401No Hit
ATCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGA329950.10640945474531183No Hit
GGCTAGTTCATAAACGAGGGGCGATGCCCGGGCTCAGAATCCAAGGAGCA328600.10597407737326706No Hit
GTTTTTCTGAGACCTATCCGAGTTCAGTGCGACCGTACAGCTCTGGAACC324590.10468084532741556No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG320790.10345533865054882No Hit
CTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAGTA320280.10329086275444299No Hit
GTCTTCTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATT316860.10218790674526293No Hit
CCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCAC313940.10124620161461795No Hit
CGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGAC311680.1005173476436393No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)