FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005284579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31007583
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA19788016.3816679939226475No Hit
GTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGT5193051.6747677495533915No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATAACACAGCA4598391.4829888546940275No Hit
TGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTG3949311.2736594142149034No Hit
GGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACC3408481.0992407889386282No Hit
AATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATA3019080.9736586047354933No Hit
GCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGG2461300.7937735746768781No Hit
GAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCAT2175120.701480021838529No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATAACAC2073930.6688460690405956No Hit
TGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAAT2052100.6618058556837533No Hit
TATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAA1968010.6346866829317203No Hit
CCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTG1822330.5877046269617339No Hit
CAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAG1704910.5498364706465513No Hit
CACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCA1701150.5486238640399672No Hit
ATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGC1587810.512071514893631No Hit
CACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGC1419180.45768804359888354No Hit
GGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGT1359150.43832826312195955No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1267200.40867422655935487No Hit
GGCTTGTTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCT1180820.38081652478363115No Hit
GTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTTACCA1093650.352704046619822No Hit
GAACGAGAGAAGGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCA1066110.3438223482301087No Hit
CACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATAACACAGCA1060900.342142114075773No Hit
CCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACT1052040.33928474850813106No Hit
CCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTC998550.3220341295224462No Hit
CCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCAT988270.31871881145976455No Hit
CCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCACGAGGGTTC973380.3139167602969893No Hit
CAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCA893560.2881746700476461No Hit
CAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATA893270.28808114453809575No Hit
ACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTT863300.2784157668787019No Hit
ACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATAACACAGCA855280.2758293027869989No Hit
GTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAG846190.2728977618152308No Hit
ATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTG809110.26093939666306787No Hit
CATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATG793070.2557664684796619No Hit
CCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAG785750.253405755617908No Hit
GTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATAACACAGCAAGACGAGAAGACCCT773610.24949058428707585No Hit
AGGAACTCGGCAAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTA772730.24920678274085406No Hit
AGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCAT746270.24067338624877666No Hit
TGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTG744030.23995098231293938No Hit
CAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATAACACAGCAAGACGAGA731800.23600678582397083No Hit
GCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATAACAC730370.23554560831136045No Hit
GTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATA691160.222900314416638No Hit
AGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAA680700.21952694603768375No Hit
CGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGG676060.21803053788487806No Hit
GGCAAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACC646600.20852963612159No Hit
GTTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTT639300.20617537329497754No Hit
CTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGC617480.19913838495570582No Hit
GGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCG615100.1983708307738788No Hit
GATGCCTTCCATGGTTTCCTCCCTTCCTTAATTCCCCTATGGTCCTACTT614720.1982482801061921No Hit
GGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATAACACAGCAAGACG581400.1875025215606131No Hit
CTTGTTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTA575900.185728761896727No Hit
TGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATAAC568010.18318422303344314No Hit
GTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATAACACAGCAAGACG553310.17844344720451122No Hit
CAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAA548140.1767761131204583No Hit
CCCTGAACAAATGGGAGCCGGAGCTACCAAGCTGCTCCAGGGTATGTCAGCCACCTCCAGATGTCCTGCATGCTG544790.17569573223427315No Hit
CTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCAC541590.1746637266116485No Hit
CGAATAGCTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAG540470.1743025246437299No Hit
GACAAACCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAA523420.168803869685683No Hit
CTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATAACACAGCAAGACGAGAAGACCCTATGG520870.16798149020515402No Hit
AATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCACGAGGGTT519870.16765898844808383No Hit
GAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTG517100.16676565858099937No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT516980.16672695837015092No Hit
GGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCACTGCCGCGTCT499910.16122185337696268No Hit
GCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAA491270.15843543819587616No Hit
AAAACATGTTGGCTTTTCTCTTGACGTGGGAGAAATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGA487110.15709383088646414No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA484770.15633917677491985No Hit
GGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATAACACAGCAAGACG481590.15531362118743663No Hit
AAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGT475160.15323993488947527No Hit
GGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAAAAATCCACTGCCTTCCAA471900.15218857916142642No Hit
GTTTACCAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCT459500.14818955737375597No Hit
CAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTTACC451600.1456417934929014No Hit
CCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAAATAGGGACCTG448880.14476458871367046No Hit
CGGGACAAGGGATCAACCTTTCCCGGCAAGGCGGAGGAATTGGGGCAAGA447720.14439048667546903No Hit
AGAACAACCGCCGCATACGGCCGATTGTCGCAGCCCGGGTCGATTATAAC447590.1443485614470499No Hit
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG440890.14218779967467957No Hit
CATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCT437950.14123964450889318No Hit
AAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTTACCAAAA434630.14016893867542013No Hit
AAAACGAATAGCTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCC434550.14014313853485452No Hit
CATAATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACC431880.13928205884347708No Hit
GCCGCGTTCGCACCAAAACCGTGAAGAAGGCGGCCCGGGTCATCATAGAAAAGTACTACACGCGCCTGGGCAACG426940.13768890016355032No Hit
AAGAAACACTGAGAAGGATACAACATTGGCCCAGAAGCCAGGGACGCTCT420040.13546363803976597No Hit
AGAGAAGGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCACTGCC412220.132941674299477No Hit
CTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGG406450.13108083916118196No Hit
AAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTATT404500.13045196073489507No Hit
ATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGA403720.13020040936438032No Hit
GGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACC395460.12753654485098048No Hit
AACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTTACCA391110.12613366220772512No Hit
AGAAGCGCAAGCGCAGCCGCAAGGAGAGCTATTCCATCTATGTGTACAAG389420.1255886342382765No Hit
AATTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACT388220.12520163212979224No Hit
CGAGAGAAGGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCA379000.12222816592960502No Hit
GGAACGTCTGAGGTTATCAATAAGCTCCTAGTCCAGACGCCATGGGTCAT373080.12031895552774945No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT368310.11878062214652461No Hit
GGAACTCGGCAAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAG361580.11661018532144217No Hit
CAAGGGATCAACCTTTCCCGGCAAGGCGGAGGAATTGGGGCAAGAGGTCC361430.11656181005788166No Hit
CCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAAGTAGGGACCTGTATGAATGGCTCCACGAGGGTTC359990.11609740752770058No Hit
TGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAA358030.11546530408384297No Hit
CAGCAATTGAAGTGAAGATGATTGACCGCACGCAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAG349500.11271436409603418No Hit
GACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT348520.11239831237410539No Hit
GGCCGATTGTCGCAGCCCGGGTCGATTATAACAACGGTGCAATCTCAGCTAAACCGACGCAGTTTTGCTCCTTGG345160.11131470647034952No Hit
CCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAG342160.11034720119913893No Hit
GCCACAGGCGTCATACTTCCCAAGAAGCGGCCATAGCCCAGATGCGAGGTGGAAAAGTCACACTAGAGCGACACC340270.10973767287827627No Hit
AATTGGGGCAAGAGGTCCCGGGGAAACGAAACTGGAAACCGACCGCCGCC337460.108831442940909No Hit
GTCATTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAA337260.10876694258949496No Hit
CCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAAGTAGGGACCTG334780.10796713823196086No Hit
GGAAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCA334320.1078187874237086No Hit
ACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCG332700.10729633457725485No Hit
AACGAGAGAAGGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCAC332390.10719635903256311No Hit
CGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGCATTCATGAAATC330440.1065674806062762No Hit
CGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTA330340.10653523043056919No Hit
AGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAAACTTCAAATTG325860.10509042255889471No Hit
CGGCAAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCC325630.10501624715476855No Hit
CTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTT322780.1040971171471185No Hit
AATTGACCTGCCCGTGAAGAGGCGGGCATAACACAGCAAGACGAGAAGAC322540.10401971672542164No Hit
AGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTG322420.10398101651457323No Hit
ACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCAC319850.10315218699890281No Hit
GGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGA315780.10183960484762711No Hit
CGGAGGAATTGGGGCAAGAGGTCCCGGGGAAACGAAACTGGAAACCGACC315290.1016815789866627No Hit
TAATTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTAC314950.10157192838925885No Hit
AGCTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTC313910.10123652656190583No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)