FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005284581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34140937
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGT605280.17728863153345792No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGTCCGCCTCTTC594490.1741282027496785No Hit
GGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTC528240.15472334575937385No Hit
CTCGTCTTGCTGTGTCATGTCCGCCTCTTCACGGGCAGGTCAATTTCACT506920.14847864310226752No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGC500030.14646053797527583No Hit
GTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGG469620.13755334248734882No Hit
GTCATGTCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG465400.13631728971000415No Hit
GGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCT453800.13291960908981496No Hit
GTCTTGCTGTGTCATGTCCGCCTCTTCACGGGCAGGTCAATTTCACTGGT411660.12057665552647251No Hit
GGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTT397490.11642621290681038No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGC351710.1030170905971327No Hit
GTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGT346670.10154085694836085No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)