FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005284584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33171471
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA25444427.670573306803306No Hit
GTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGT9667172.9143024739542No Hit
TGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTG6025801.8165609839853047No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCA5723691.7254857344131649No Hit
GCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGG5061101.5257387892143826No Hit
GGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACC3718791.1210808227346927No Hit
CCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTG3516321.060043433105514No Hit
CAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAG3207330.9668941121121821No Hit
GGTTTGTTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCT3189190.9614255575220044No Hit
AATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATG3079190.9282645318924807No Hit
GTTTGTTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTT2906940.8763373803953404No Hit
GTTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTT2806400.8460282029699556No Hit
TATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAA2683700.8090385861995688No Hit
CACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCA2550630.7689227891039261No Hit
GGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGT2477510.7468797509763736No Hit
TGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAAT2351050.7087566300571958No Hit
CACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGC2282340.6880430475935179No Hit
ATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGC2218250.66872222820628No Hit
CCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCACGAGGGTTC2130200.6421783345091931No Hit
GAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATT2085160.62860040183325No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2019880.6089208404414745No Hit
CCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTC1935060.5833506750424182No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACAC1833740.5528063557989333No Hit
CCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACT1822250.5493425359399949No Hit
GTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTTACCA1814480.5470001616750731No Hit
GTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAG1780760.5368348000002773No Hit
GGGTTTGTTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTC1635010.4928964410411585No Hit
ACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTT1516360.45712775294167696No Hit
AGGAACTCGGCAAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTA1448970.43681210278555327No Hit
CAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCA1304830.39335910065610297No Hit
ATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTG1285820.38762827249958254No Hit
CAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATA1269650.3827536017320426No Hit
CTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGC1159320.34949309302563036No Hit
CCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCAT1158590.3492730244009981No Hit
GTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATA1142090.34429887055656955No Hit
CGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGG1124250.3389207551271995No Hit
GGCAAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACC1091470.3290387694896014No Hit
AGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCAT1087780.3279263678116656No Hit
AGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAA1052780.31737513238408993No Hit
CATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATG1023540.30856032884402385No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT1022760.3083251870259236No Hit
GTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCAAGACG1004180.30272398833322767No Hit
ACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCA984730.2968605160741892No Hit
GGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCAAGACG984600.2968213257711725No Hit
AATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCACGAGGGTT983500.2964897155148772No Hit
GTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCAAGACGAGAAGACCCT974660.29382477490974096No Hit
AAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGT973600.29350522320822015No Hit
AATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCAAGACGAGAAGAC961180.28976104195077756No Hit
CAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAA953130.2873342578024351No Hit
CAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCAAGACGAGA941020.2836835303444939No Hit
CACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCA936130.2822093720233269No Hit
GTTTACCAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCT922440.278082331651798No Hit
GCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACAC921940.2779315997171184No Hit
GTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCAAGACGAGAAGACCCTATGGAGCTTTAATTTATT918150.2767890516522466No Hit
CCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAG912040.27494710741046124No Hit
TGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTG901910.27189327841385147No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA888460.2678385893709688No Hit
CCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAAGTAGGGACCTG857630.2585444582786214No Hit
GGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCG857010.25835755067961863No Hit
GAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTG852090.2568743484423709No Hit
GGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGA843900.2544053593523181No Hit
CCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAAATAGGGACCTG829350.2500190600531403No Hit
GTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTT826880.24927444429582277No Hit
CTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGG822280.24788771049676994No Hit
GGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCAAGACG808880.24384809464735527No Hit
CAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTTACC802330.2418735063030518No Hit
GGAACTCGGCAAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAG795830.23991399115221632No Hit
CTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCAAGACGAGAAGACCCTATGG795340.23976627385623026No Hit
AACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTTACCA778950.23482528103743122No Hit
CATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCT761990.2297124538130974No Hit
TGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAA750320.22619437045767432No Hit
GGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACC725930.21884166668400085No Hit
AGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCC712160.2146905092029232No Hit
GTCATTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAA674680.20339164337933643No Hit
GGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA657850.19831800645801928No Hit
AGCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCACGA651190.19631025708808633No Hit
AATTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACT644780.1943778736854932No Hit
ACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCACGAGGGTTC621300.1872995020329367No Hit
GTACTGTTTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTG618110.18633783228968048No Hit
AGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTG614980.18539425037858587No Hit
AAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTATT612930.18477624944639928No Hit
CATAATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACC612180.18455015154437981No Hit
GAACTCGGCAAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGC601310.18127323928444414No Hit
ACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCAC592630.17865653289840536No Hit
GGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAA581180.17520477159424133No Hit
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG563120.16976033411361227No Hit
GGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCA555290.1673998720165289No Hit
CTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAA554690.16721899369491333No Hit
TGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGAC550270.1658865233923452No Hit
GCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAA539010.16249204022335942No Hit
GACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT537510.16203984441932046No Hit
ATTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTT537240.16195844917459346No Hit
GTACTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAG521060.15708076376835986No Hit
AAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTTACCAAAA519450.15659540693869137No Hit
GAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATC517200.15591711323263294No Hit
CTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAG494730.14914322008812933No Hit
CCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAAATAGGGACCTGTATGAATGGCTCCACGAGGGTTC488440.14724701234985932No Hit
CTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCC483360.14571557589351403No Hit
ACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCG473110.14262557123258116No Hit
GGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGG469820.14163375510238904No Hit
GTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAAT465220.14024702130333622No Hit
CCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAAGTAGGGACCTGTATGAATGGCTCCACGAGGGTTC463290.13966519603547276No Hit
TAATTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTAC457620.1379558958962055No Hit
ATAATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACC449370.13546881897399124No Hit
GGTACTGTTTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTT446970.1347453056875289No Hit
AGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGA444510.134003704568905No Hit
CGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAAGTAGG433160.1305820896516769No Hit
CGGCAAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCC428940.12930991212298062No Hit
CTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGT422470.1273594408882259No Hit
CCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAG419470.126455049280148No Hit
AAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTATT417040.12572249207760489No Hit
CTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAA416770.12564109683287786No Hit
ATCACTTGTTCCTTAAGTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTG415890.12537580862784167No Hit
AGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTTACCAAAAACATCA411700.12411267501522619No Hit
AGTCATTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAA410460.12373885981722064No Hit
CACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAA392720.11839089077478657No Hit
AACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTAT383340.11556315968019627No Hit
GAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAAC383100.11549080835155004No Hit
AGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACG377850.11390812303741368No Hit
CTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGT377800.11389304984394573No Hit
CATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGT373500.11259675520570071No Hit
AAGGAACTCGGCAAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCT371570.11201492993783725No Hit
GGAGAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAA371150.11188831511270633No Hit
ATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGA370430.11167126112676765No Hit
GACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATTATTACCC370220.1116079537142022No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGG362870.10939219427441128No Hit
AGGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGA356680.10752613292307718No Hit
TTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTC343920.10367945395005244No Hit
GGTACTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGA341180.10285344294800795No Hit
ATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCC341160.10284741367062075No Hit
CTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCTAAAC335180.10104465973185212No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)