FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005284586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32625042
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAAAAATCCACTGCCTTCCAA4370641.3396580454976885No Hit
CTGACAAACCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAAAA2008410.615603805199699No Hit
TGACAAACCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGA1390530.42621554326274885No Hit
GGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAAAAATCCACTGCCTTCCAA1353610.4148990827352804No Hit
GTCTGACAAACCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAA1245880.3818784355894469No Hit
CCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGAC1050410.3219643364750305No Hit
CGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAAAA789350.2419460486824814No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCA690770.21172999562728534No Hit
CCTCGCTTCGCTTTTCCTCCGCAACCATGTCTGACAAACCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGA654640.20065568038195933No Hit
CAGCATTCGGGCCGAGATGTCTCGCTCCGTGGCCTTAGCTGTGCTCGCGC641790.19671698813445207No Hit
CAAACCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAAAAATCC591340.18125340650902458No Hit
GACAAACCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAA571220.17508636463977578No Hit
CCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACA523120.1603430886004683No Hit
CCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTT519850.1593407910402077No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA501510.1537193423383179No Hit
CCGCAACCATGTCTGACAAACCCGATATGGCTGAGATCGAGAAATTCGAT493610.15129788951689319No Hit
CTTTTCCTCCGCAACCATGTCTGACAAACCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGA484680.14856072828963715No Hit
CTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAACCATGTCTGACAAACCCGATATGGCTG464990.14252548701699755No Hit
CCATGTCTGACAAACCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCA462410.14173468343734238No Hit
CAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAG439290.13464810252198295No Hit
CACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTT416780.12774849454599937No Hit
CATGTCTGACAAACCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAG399890.12257148971639636No Hit
GCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAAAAATCCACTGCCTTCCAA381290.11687034763051034No Hit
GTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGT375960.11523663325858707No Hit
CCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAACCATGTCTGACAAACCCGATATGGCTG359990.11034162040312469No Hit
CTGCTGAGAGCAAGATGGGTCACCAGCAGCTGTACTGGAGCCACCCGCGA359640.11023434084774511No Hit
ACCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGA327130.10026960271805935No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCAA1121700.029.3710867
CACGCGC124600.029.21046470
CTGCCTT1134000.028.68383263
ACTGCCT1163100.027.94336762
CCTTCCA1213100.026.83013766
TGCCTTC1259050.025.88665664
GCCTTCC1262900.025.85403365
CGCCTAA37750.025.63945449
GCTCTTG177300.025.54380670
AACGATT371550.025.40880670
CGCGGTC29500.025.0141661
TGCCGAC158000.024.89931370
CACTGCC1317150.024.85130161
CATTCGG201100.024.7654024
GCGGTCG29350.024.663352
ATCCACT1319650.024.65198158
CTTGACG41900.024.4740641
CGCTAAC21200.024.16859470
ACGTCTG51100.024.1283534
AATCCAC1359050.024.06823757
Produced by FastQC (version 0.11.5)