FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005284589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3549627
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT91666725.82431900591245RNA PCR Primer, Index 3 (100% over 34bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA2572627.247578407534087RNA PCR Primer, Index 7 (97% over 35bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAG972652.740147063339331RNA PCR Primer, Index 7 (97% over 36bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC627051.7665236375540305RNA PCR Primer, Index 1 (100% over 33bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGC483871.3631573120217984RNA PCR Primer, Index 10 (100% over 50bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA474351.3363375926541015RNA PCR Primer, Index 1 (100% over 32bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA278360.7841950717638781No Hit
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGC214320.60378174946269RNA PCR Primer, Index 10 (100% over 37bp)
CACCACGATCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT126600.3566571924317682RNA PCR Primer, Index 3 (100% over 34bp)
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCG105120.2961437920096957RNA PCR Primer, Index 10 (100% over 45bp)
CGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGT92800.26143591988679377RNA PCR Primer, Index 10 (100% over 46bp)
GTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATG91320.2572664677161854RNA PCR Primer, Index 10 (100% over 49bp)
CACCACGTTCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA82580.23264416233029556RNA PCR Primer, Index 7 (97% over 35bp)
CACCACGAACCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT81920.23078481203799725RNA PCR Primer, Index 3 (100% over 34bp)
CACCACGTACCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT76070.21430420717444398RNA PCR Primer, Index 3 (100% over 34bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA70650.19903499719829718RNA PCR Primer, Index 1 (100% over 28bp)
ATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT68330.19249909920112734RNA PCR Primer, Index 1 (100% over 30bp)
CGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTC68180.19207651958924135RNA PCR Primer, Index 10 (100% over 44bp)
CGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCG61180.1723561377012289RNA PCR Primer, Index 10 (100% over 45bp)
CCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTA56760.1599041251376553RNA PCR Primer, Index 10 (100% over 47bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG54500.15353725898523987RNA PCR Primer, Index 1 (100% over 29bp)
CCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGT51480.14502932279926878RNA PCR Primer, Index 10 (100% over 46bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50060.14102890247341482No Hit
CCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTA48820.13753557768182403RNA PCR Primer, Index 10 (100% over 47bp)
TCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTC46820.13190118285667762RNA PCR Primer, Index 10 (100% over 44bp)
GCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCG46630.1313659153482887RNA PCR Primer, Index 10 (100% over 45bp)
CCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTC46220.13021086440913368RNA PCR Primer, Index 10 (100% over 44bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCG46150.13001366059025357RNA PCR Primer, Index 10 (100% over 45bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA42990.12111131676652222No Hit
CGCGACCTCAGATCAGACGTGGCGACCTGGAATTCTCGGGTGCCAAGGAA40800.1149416544329869RNA PCR Primer, Index 1 (100% over 23bp)
CGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTC39120.11020876277986391RNA PCR Primer, Index 10 (100% over 44bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTC36790.10364469280856833RNA PCR Primer, Index 10 (100% over 44bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGCT150.002222263570.024011
CACGCCG150.002222263570.024011
GCCTACG150.002222263570.024011
ATACGAC150.002222263570.024011
CCGATTA150.002224499870.0062566
CTCCCGC150.002224499870.0062566
CCCTTAC150.002224499870.0062566
CCAGGTA150.002224748570.004283
TTACTTC150.002224748570.004285
GGCCAAT150.002224748570.004283
CACTAAG150.002224748570.004283
TCCTACG550.069.99541511
ATCTACC150.002225867569.9954122
TTTAAGG207.933473E-569.9954110
CATGAGG252.843326E-669.9954112
TCTAACC252.843326E-669.9954112
GCGATCA150.002225867569.9954122
TCCTCAA252.8435625E-669.99441514
TCGATAC150.002225991869.99441514
TCGTACA252.8435625E-669.99441515
Produced by FastQC (version 0.11.5)