FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005284602

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284602
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3492707
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA101691829.11546831726795No Hit
TCTTAACCCGGACCAGAAACTATGGAATTCTCGGGTGCCAAGGAACTCCA1734774.966835179704453RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTATGC1535934.397534634310865RNA PCR Primer, Index 16 (100% over 50bp)
AGGTTCCGGATAAGTAAGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCA1416204.054734622743906RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA1148733.2889389233050466No Hit
GTCCCACTCCGTAGATCTGTTCTGGAATTCTCGGGTGCCAAGGAACTCCA797002.2818976799370803RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATGTCGTATGC284470.8144685483208297RNA PCR Primer, Index 16 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCAGCTCGTATGC223110.6387881949444943RNA PCR Primer, Index 16 (98% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG167450.47942756148740795No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGAATTCTCGGGTGCC161180.4614758695762341No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG149430.4278343416725193No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTTGGAATTCTCGGG145100.41543708075140573No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT124180.35554084553900456No Hit
CCTGGAACTTAGGACGTGAATCTGGAATTCTCGGGTGCCAAGGAACTCCA116110.3324355578638575RNA PCR Primer, Index 1 (100% over 28bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCG115750.3314048387110628RNA PCR Primer, Index 7 (97% over 36bp)
TAGCATATCGAGCCTGAGAACATGGAATTCTCGGGTGCCAAGGAACTCCA112960.3234167652769041RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC111450.31909347105268204No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCAGGTCGTATGC107470.3076982981967855RNA PCR Primer, Index 16 (97% over 40bp)
TGATACGGATGTTATACGCAGCTGGAATTCTCGGGTGCCAAGGAACTCCA102350.2930391813570391RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCT99510.2849079524849923No Hit
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC88800.254244057689351RNA PCR Primer, Index 2 (100% over 34bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA77530.22197682198936242RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCGCGTATGC74440.21312981592787483RNA PCR Primer, Index 16 (98% over 50bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACATGGAATTCTCGGGTGCCA60850.1742201679098762No Hit
TTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCC58640.16789269755522007No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAATGGAATTCTCG55500.15890253605584437No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC54820.15695562210056554No Hit
AGATTTCAACTTAACTTGACCGCTCTGACCATGGAATTCTCGGGTGCCAA53670.15366304702913813No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGTGGAATTCTC51790.14828040256454378No Hit
ATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGC46180.13221836243349355No Hit
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG45850.13127353654343177RNA PCR Primer, Index 1 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTTGGAATTCTCGGGT45250.12955567128877402No Hit
TGTAGTATTCTCTGATGGTAGTTTGTATTTCTGTGGAATTCTCGGGTGCC39970.11443845704778557No Hit
GCTCTACACTCTACTCGTCAGCTGTTGGAATTCTCGGGTGCCAAGGAACT38770.11100272653847001RNA PCR Primer, Index 1 (100% over 25bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCC38650.11065915348753845RNA PCR Primer, Index 5 (97% over 35bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCCTCTCGTATGC37180.1064503836136269RNA PCR Primer, Index 16 (98% over 50bp)
TGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA35300.10106773914903254No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGACG150.002223767370.012041
CTAGACG207.927495E-570.006033
CTATGCG150.002224525270.006033
CTCACGG207.927495E-570.006036
CTTCCGA207.927495E-570.006035
AGCTTCG150.002224525270.006036
CTAACGG207.927495E-570.006035
GTACGAT1650.070.006036
GATACGT207.928057E-570.005032
GAGACGG150.002224651670.005032
GCTAGAC252.8409959E-670.005022
ACGTAAC150.002225156970.0010265
GCGCTCA150.002225156970.0010236
TCGCTAT207.930306E-570.00101536
TTCGCCC150.002225409569.9990167
CTCCGTT207.931431E-569.999017
CGATAAG207.931431E-569.999018
TTAGCGA150.002225662369.9970119
TAGCGAG2050.069.9970118
GCTTATA301.0252006E-769.9970120
Produced by FastQC (version 0.11.5)