FastQCFastQC Report
Wed 8 May 2024
EGAF00005284605

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284605
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4129553
Total Bases313.8 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA76944118.632549334032035No Hit
TCTTAACCCGGACCAGAAACTATGGAATTCTCGGGTGCCAAGGAACTCCA3815549.239595665681007RNA PCR Primer, Index 1 (100% over 28bp)
AGGTTCCGGATAAGTAAGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCA3271107.921196313499306RNA PCR Primer, Index 1 (100% over 28bp)
GTCCCACTCCGTAGATCTGTTCTGGAATTCTCGGGTGCCAAGGAACTCCA2070985.0150222070040025RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTATGC1728764.186312659021449RNA PCR Primer, Index 16 (100% over 50bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA870582.1081700610211325No Hit
TAGCATATCGAGCCTGAGAACATGGAATTCTCGGGTGCCAAGGAACTCCA291630.7062023419968214RNA PCR Primer, Index 1 (100% over 28bp)
CCTGGAACTTAGGACGTGAATCTGGAATTCTCGGGTGCCAAGGAACTCCA276640.6699030137160124RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATGTCGTATGC264880.6414253552382062RNA PCR Primer, Index 16 (98% over 50bp)
TGATACGGATGTTATACGCAGCTGGAATTCTCGGGTGCCAAGGAACTCCA252540.6115431863932973RNA PCR Primer, Index 1 (100% over 28bp)
TCTTACTCGTCTATCGGCTAGGATGCGCGCCTTGGAATTCTCGGGTGCCA189180.4581125366353211No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCG183940.4454235119394278RNA PCR Primer, Index 16 (100% over 36bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA170440.41273232236031354RNA PCR Primer, Index 1 (100% over 28bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC150320.36401034203944105RNA PCR Primer, Index 2 (100% over 34bp)
TTCTTACTCGTCTATCGGCTAGGATGCGCGCCTTGGAATTCTCGGGTGCC114750.2778751114224712No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGAATTCTCGGGTGCC102920.24922794307277327No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG97670.2365147026808955No Hit
CTTACTCGTCTATCGGCTAGGATGCGCGCCTTGGAATTCTCGGGTGCCAA97040.23498911383387014No Hit
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG89440.21658518488562808RNA PCR Primer, Index 1 (100% over 29bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCAGCTCGTATGC82890.2007239040157615RNA PCR Primer, Index 16 (98% over 50bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT80490.19491213697947454No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTTGGAATTCTCGGG75000.18161771988396808No Hit
GCTCTACACTCTACTCGTCAGCTGTTGGAATTCTCGGGTGCCAAGGAACT73010.1767987963830468RNA PCR Primer, Index 1 (100% over 25bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC68030.1647393797827513No Hit
TGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA68020.16471516408676676No Hit
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC59350.14372015566818006RNA PCR Primer, Index 1 (100% over 27bp)
ATAGGCGATAGAAATTGAAACCTTGGAATTCTCGGGTGCCAAGGAACTCC56630.13713348636038816RNA PCR Primer, Index 1 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACATGGAATTCTCGGGTGCCA54180.13120064084417854No Hit
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCC53280.1290212282055709RNA PCR Primer, Index 16 (100% over 35bp)
TATGCATATGATCACGAGACTCAGTTGGAATTCTCGGGTGCCAAGGAACT51870.12560681507175234RNA PCR Primer, Index 1 (100% over 25bp)
CGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA51070.12366955939299No Hit
AGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA50110.12134485257847522No Hit
CGATGCTATAGACTCTCACGTGATGTGGAATTCTCGGGTGCCAAGGAACT46980.11376533973531759RNA PCR Primer, Index 1 (100% over 25bp)
AGATTTCAACTTAACTTGACCGCTCTGACCATGGAATTCTCGGGTGCCAA45270.10962445572196312No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC43750.10594366993231472RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC42840.10374004159772257No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)