FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005284606

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284606
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39165215
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACT3285410.8388591764401141No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGT2819090.7197943379092903No Hit
GGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTC2332260.5954927095382982No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT2248410.5740834053891954TruSeq Adapter, Index 6 (97% over 36bp)
GTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGG2072170.529084290741159No Hit
GGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGAC1858360.47449248012554No Hit
CGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATT1809010.46189201310397504No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG1674460.42753754830657764No Hit
GTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCC1651340.42163435078806544No Hit
GGGCTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCT1639450.41859849358671974No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGC1601600.4089343056076674No Hit
GTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGT1522190.38865866049758696No Hit
CTCCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA1509710.3854721594149298No Hit
CACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACA1459150.37256274477237006No Hit
GCTCCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGT1301780.3323816810401781No Hit
AGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGAC1276910.32603165844997917No Hit
CTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGG1253840.3201412273620865No Hit
GTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATT1166670.297884232219841No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGC1146970.29285425855571073No Hit
GGCTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTT1085440.27714388903520637No Hit
CCTAAATCAAGACACAGCACCCTTTAATTATACAGACGATGGACAACAATAAAGTGTACAGACCACAGCTCAAAA1078350.27533360917334426No Hit
GGGACTCTTTTGCCAACTTGGGAATGTTCGCGAATATATGCTGAAGCCGT1051550.26849080236122796No Hit
CTACAGAAAAAATTCAAACAGGTCCCCGAGGCGTTTTGAAATTCATCCCA1046380.2671707534351592No Hit
GATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGGGAAT1043320.26638944788123853No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC967680.24707639163987738No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTC939660.23992208392064232No Hit
GTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGTTTCGTTTCCCCGGGACCTCTTGCCCC904150.23085536489458824No Hit
AGGACCTGTGGGCTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCC885250.22602965412037188No Hit
GTCATTTAGAATTAATCGAGTCAAGAAATAGCGTAGCACCATTATCATCATAAGTAAACAAAAGGCGTCAGACAA882160.22524068870807937No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTCTAATACTGGTG868170.2216686414207097No Hit
GGACTCTTTTGCCAACTTGGGAATGTTCGCGAATATATGCTGAAGCCGTG857770.21901322385182873No Hit
GTGAAAATTAACAAGAAGCAATACAGAGTATGGTTTAGGGCCCTGGATCG852920.2177748800817256No Hit
GGACCTGTGGGCTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTT850080.21704974682253117No Hit
GGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTG822780.21007927570421864No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCGCGTAT802030.20478120699707636TruSeq Adapter, Index 6 (97% over 36bp)
CCCTGGAGGTGATGGTCGAGCGCTTGTTGTAATGCGCCAGGCGGGAAGCC801620.20467652226599545No Hit
GCCTACAGCACCCGGTATTCCCAGGCGGTCTCCCATCCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCG800930.2045003455234447No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA792470.202340265462605No Hit
TGTTCATATACACCGTAGGGCTTGGCTGTTCTATTGGTAGTAAACGTAAA731820.18685458512100597No Hit
CACGGCTTCTATTCGATGATCCGATATGGTAAAATTTATTCAGACTGTAAAGTAGGACCATAGGGGAATTAAGGA715990.18281273318683428No Hit
GCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG698990.1784721467761635No Hit
AAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGA674870.1723136206452588No Hit
CCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGCTTGTTAGGTACTGTTTGCATTAATAA673730.17202254602713146No Hit
GGGAACAATTAGCGCCAAAGAAACACAGACCAGGATAAGGAAGATGGGAACGGAGGAGCATAGTGAAACATAATT668500.1706871773843192No Hit
GTGGGCTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGT659610.1684173060201508No Hit
GGGTATAAGGGGAGTGGTGTCTAAATTGACCCTGACAACTTACAGTGCGT654730.16717130239167588No Hit
ATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG654620.16714321624431272No Hit
CTGTGGGCTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGG653420.16683682190944182No Hit
CACTAGTGAAGTTTGAGAAAACCTTCTAAAACACACCTGGATCGTGCGAG650100.16598913091629908No Hit
CTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTA633290.16169705694198283No Hit
GTGGTGTCTAAATTGACCCTGACAACTTACAGTGCGTCTAGGTCGGGGCA623590.15922036940177656No Hit
CATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG620400.1584058711282448No Hit
CCTGACTCGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGAC611680.15617940562818308No Hit
ATTGCGTGATGCCCCAGCATGCCGCCTTGTTACCCACAACAAAACAGCTCTTCTTTTTCCCTTGTCTCTCTTCAA610100.15577598642060309No Hit
GCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCG595260.15198690981269986No Hit
GTTGCACTTATGAGAATATTGGCCCAGCTCAACTTCTACTGGGTTATATA585310.14944639011939548No Hit
GTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCG579700.14801399660387413No Hit
CCCGAGGCGTTTTGAAATTCATCCCAACTGTAGGCTGAGTGACCTGAAGGTTGGACAGACTGCCGAAGTCCAAA576680.14724290419444908No Hit
GTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCA569110.14531006659863863No Hit
GGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCG554760.14164610101080768No Hit
GTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTT551860.1409056480348697No Hit
CATCCGGCTGCATAAAAATCAAGCTCAAGAACTCGGGAACAATTAGCGCCAAAGAAACACAGACCAGGATAAGGA540510.13800766828421598No Hit
CTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATG539660.13779063896368243No Hit
CTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTG531610.1357352436339236No Hit
CTCCTGATCCAAGGCTGAGACCTCAGGAACATAATTGTCTCTCCTTTCTCTCTCCTCCTCCTGCAGCTTGATGG529450.13518373383115603No Hit
GTGGATCTTTAGCAACTCGAACGGGACGCCGTTCTGGGGTATCGTGGGAC519810.1327223660076933No Hit
GGGGCGACACGCAGAGTATGGTTTAGGGCCCTGGATCGTGCGAGAGAAAG511260.1305393063717383No Hit
AGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGA505810.1291477654341997No Hit
GCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTTATG497920.12713322268242366No Hit
GTTCGATCCTATTTATTGTGAACAACATGGGTTGCTTTTGAAGGCCGGAA495430.1264974544375666No Hit
GGCTTCTATTCGATGATCCGATATGGTAAAATTTATTCAGACTGTAAAGT486220.12414587791743259No Hit
GTTCTTTTGTTACCACCAACCTTATTGCAGATCGCGCAAACCCAAATGCG481100.12283859542198351No Hit
CGGACTCTTTTGCCAACTTGGGAATGTTCGCGAATATATGCTGAAGCCGT476320.12161812465474783No Hit
CCCAGATTTATTGAAAATAATACAGCACTACAGAAAAAATTCAAACAGGT464550.1186129068868893No Hit
CCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCAC458850.11715753379625263No Hit
GGCGTTTTGAAATTCATCCCAACTGTAGGCTGAGTGACCTGAAGGTTGGACAGACTGCCGAAGTCCAAAAGCTTC449500.11477021127038367No Hit
CCCTCGTGGAGCCATTCATACAGGTCCCTAATTAAGGAACAAGTGATTAT444150.11340420319408434No Hit
ACATGAGCTGCCTACTCATTTTCTTCACTGCGCAGCCTGGCATTGGGGTTGGTGACTCTGATGGCCAGTTGGGC443970.11335824404385371No Hit
TGTTAATTGGGAAATGAAGCAATAATGATGTGGCTTATGCTTACTGGTAA442590.11300589055875221No Hit
CTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGA439890.1123165033052927No Hit
GTCCATGTTGCATTACCAGCCTAAAATTGCCAAATATGATATAGGATGAC439690.11226543758281424No Hit
GTTGTCCACAGTCAGCAATGGTGATCTTCTTGCTGGTCTTGCCATTCCTG434450.11092751565387807No Hit
GTTGCTTTTGAAGGCCGGAATGTAGCTTACGCATACACTCTTTTTCCCGT432450.11041685842909327No Hit
CGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGG429570.10968151202540315No Hit
AAAAAATTCAAACAGGTCCCCGAGGCGTTTTGAAATTCATCCCAACTGTAGGCTGAGTGACCTGAAGGTTGGAC428270.10934958482929304No Hit
GGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAAT425030.10852232012514165No Hit
GGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGAC423470.1081240074898095No Hit
CGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGGGAATGTT423120.10803464247547216No Hit
GGGTAAAACGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGCCTACCAGT421750.1076848422764946No Hit
GAGGTGAAGGGAGGTGGGGTATAAGGGGAGTGGTGTCTAAATTGACCCTG420170.1072814230689146No Hit
CTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTGTCGATGATCAATGTGTCCTGC417930.10670948697715563No Hit
GCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGG417770.10666863439917285No Hit
TATCACGATTGATAGACTTTACATTCGCGAGAGCTACTGATTCTATGAAG415590.10611201802415741No Hit
GTGCCATTCGGACCTACCGTAAGCCTATATTTCGTTTTTCTGAGACCTAT414210.1057596645390559No Hit
CTAGGCTAATTTATATACGTCACGGCTTCTATTCGATGATCCGATATGGT413290.10552476221565488No Hit
AGCTCCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGG411500.10506772399947249No Hit
CCAGAATTCAAGACGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCT410910.10491708011816096No Hit
GCGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGAT410900.10491452683203704No Hit
CAGCACTACAGAAAAAATTCAAACAGGTCCCCGAGGCGTTTTGAAATTCATCCCAACTGTAGGCTGAGTGACCTG407900.10414854099485985No Hit
CGGCTAATTTATATACGTCACGGCTTCTATTCGATGATCCGATATGGTAA405720.10359192461984441No Hit
ACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCCCTAATTAAGGAACAAGTGATT404400.10325489085148645No Hit
GGTAGTTTAGGACCTGTGGGCTTGTTAGGTACTGTTTGCATTAATAAATT403310.10297658266397874No Hit
GTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGG402640.10280551249367584No Hit
GCCTTCTCTCGTTCTCGCCCGCTTTGCAAAAATATCTAATATCAATTGCG398060.10163610744891864No Hit
GGCTGGAGCCGTGTCTCTGTGGACGGGAAAGGGCGAATCATAGGGAGGTG397960.10161057458767939No Hit
CGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTC395630.10101565892080512No Hit
AGGTTATCTAAATGGTTTTAAGCGTGGCCCGTATCTGCAGGGGCGACACG392460.1002062672195212No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)