FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005284607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39165215
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA5834581.4897352152924477No Hit
CAAAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTT3615370.9231074053851103No Hit
GTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGT3108890.7937885697806075No Hit
CAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAG2535970.6475057011687539No Hit
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG2477490.6325740839160464No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATAACACAGCA1627800.41562391525234826No Hit
ATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGT1565710.3997705617089042No Hit
GGAGAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAA1282000.32733128108705645No Hit
CCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTG1280270.3268895625876176No Hit
CACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCA1181980.30179331327556863No Hit
CGTATATAAATTAGCCTAGTAACTTCCTTCCTGCCCCGACCTAGACGCAC1158720.29585436975132146No Hit
GGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACC1155150.2949428466050806No Hit
TGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTG1133190.28933583027694343No Hit
CACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGC1122910.28671105214154957No Hit
GGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGT1077430.27509870684994325No Hit
GGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAA1028870.2626999494321683No Hit
AGGAGAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGA991050.25304342131148777No Hit
CCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACT926600.2365874922427976No Hit
GCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGG872980.2228967720463171No Hit
CCTCCCTATGATTCGCCCTTTCCCGTCCACAGAGACACGGCTTCAGCATATATTCGCGAACATTCCCAAGTTGGC870190.22218440521774233No Hit
AGGAGATTCAACCCATGCACCAGACCACGAAACTACCGAGTAGTCTGAGA865890.22108649218445503No Hit
AATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATA819990.20936690887564385No Hit
GTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAG807970.20629785895468722No Hit
TGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAAT780370.19925078925265696No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATAACAC753500.19239010943767318No Hit
CGAATAGAAGCCGTGACGTATATAAATTAGCCTAGTAACTTCCTTCCTGC714640.18246803956010454No Hit
CCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTC709490.1811530972062837No Hit
CATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATG705040.1800168848811375No Hit
GTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTTACCA700650.17889599227273487No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG690410.1762814272818367No Hit
CCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCAT677670.17302854075995752No Hit
TGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTG669720.17099867829143794No Hit
CTGGGGCATGGTGGGTTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCT669380.17091186656322455No Hit
CCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCACGAGGGTTC664100.16956373148979265No Hit
GGGCTATTGTATGTTAGTTCAGAGGTGCTTAGCAGATTGTCTCAGCTCAGGAATCGGCGTATATTTGATAAATCA660660.16868540106316282No Hit
CAGAGAGGCCCAGTAAGAGGTATCTCCATCAAGCTGCAGGAGGAGGAGAG655320.1673219462729874No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG653330.16681384233432653No Hit
CCCCACCTCCCTTCACCTCCCTATGATTCGCCCTTTCCCGTCCACAGAGA646770.16513888663703238No Hit
GATCGTGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTTAATTCCCCTATGGTCCTACTTTACAGTCTGAATA640460.16352776309283631No Hit
GGCGCAGAAGAAGGACGGCAAGAAGCGCAAGCGCAGCCGCAAGGAGAGCT639990.1634077586450119No Hit
CACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGATACCAGATGTGAC603240.15402443213959122No Hit
GGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCACTGCCGCGTCT597810.15263799777430048No Hit
AGTAAGAGGTATCTCCATCAAGCTGCAGGAGGAGGAGAGAGAAAGGAGAG579600.14798846374263488No Hit
CAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTC572420.14615520430565745No Hit
TATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAA566260.14458238005332028No Hit
AGAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGA557100.14224356996380588No Hit
CCCTTTCCCGTCCACAGAGACACGGCTTCAGCATATATTCGCGAACATTC554330.14153630970747894No Hit
AGGAGATCGCCATTATCCCCAGCAAAAAGCTCCGCAACAAGATAGCAGGT548490.14004519061110735No Hit
CGATGAAGAACGCAGCTAGCTGCGAGAATTAATGTGAATTGCAGGACACA542580.13853619851186824No Hit
ATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGC541410.13823746403536913No Hit
ACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTT537110.1371395510020818No Hit
CACATTTTCTTTTTCTACCTTCGCCCTTCGATCGGGTGTTAGGGGGAAGT533350.1361795154194864No Hit
CTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGC531340.13566630490857767No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT530510.135454382160292No Hit
CCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTTAATTCCCCTATGGTCCTACTTTACAGTCTGAAT524120.13382283232710454No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT522170.13332494153293936No Hit
ACAGCATTCATCTGACGAACTGGGGACTCCGATTATCACGTCTTGATTTTGAAGAGAGACAAGGGAAAAAGAAG509630.13012312073353868No Hit
CCTGAATCTGTTGCCTCCAGGTGTTAACACAGAATTAAGCATCCACAATT502760.1283690131664029No Hit
TGATCAGCGTCGGCGCAAATCAAATAATTTTTTTTTTGATTTGTTCGACT498750.12734514543070938No Hit
CAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCA492420.12572891531426547No Hit
CTTCCTTAATTCCCCTATGGTCCTACTTTACAGTCTGAATAAATTTTACC476740.12172536267195265No Hit
CAGCAATTGAAGTGAAGATGATTGACCGCACGCAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAG467940.11947847088289952No Hit
TGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAA465370.11882227634905107No Hit
GATTATCAGACGATCAGGGAATTTACCTACAGAGGACTCGGGATCCCTGG463080.11823757382667247No Hit
GGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGCTTGAAGC459280.11726732509958135No Hit
CTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCAC457440.11679752045277933No Hit
AAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAGC456430.11653963855426301No Hit
GGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCG445430.1137310238179466No Hit
AGGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGA445410.11372591724569878No Hit
GAGGTGGCAGCCATCTCCTTCTCGGCATCATGGCCGCCCTCAGACCCCTTGTGAAGCCCAAGATCGTCAAAAAG439420.11219649885746828No Hit
CACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATAACACAGCA436540.11146115245377819No Hit
GTAAGTTGTCAGGGTCAATTTAGACACCACTCCCCTTATACCCCACCTCC435120.11109858582418097No Hit
GAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCAT433880.11078197834481439No Hit
GTATCTCCATCAAGCTGCAGGAGGAGGAGAGAGAAAGGAGAGACAATTATGTTCCTGAGGTCTCAGCCTTGGATC432510.11043217814583682No Hit
CGCGTTCGCACCAAAACCGTGAAGAAGGCGGCCCGGGTCATCATAGAAAA432300.1103785591372344No Hit
GAGGAGAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGG429220.10959214701106582No Hit
CGGGTCCTGGCATCTTGTCCATGGCAAATGCTGGACCCAACACAAATGGTTCCCAGTTTTTCATCTGCACTGCCA426110.10879807502652546No Hit
CTTCACCTCCCTATGATTCGCCCTTTCCCGTCCACAGAGACACGGCTTCA420680.10741164066123472No Hit
AGGAGACATAACGATAGCGACTCTGTCTCAGGGGATCTGCATATGTTTGC420270.10730695593015382No Hit
GCCATCATGAACGACACCGTAACTATCCGCACTAGAAAGTTCATGACCAA419880.1072073777713208No Hit
GGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACC417750.106663527826925No Hit
TGGAAAAAAAGATTTACACAAGCAAAAGGAAGGCTGATCGGAAAAGTATA414410.10581073026153438No Hit
GTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACG407310.10399789711354834No Hit
CAGAGACACGGCTTCAGCATATATTCGCGAACATTCCCAAGTTGGCAAAA406430.10377320793464302No Hit
TGTCAAAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAG404890.10338000187155873No Hit
GGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGA402020.10264720875399255No Hit
CCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTAT401400.10248890501430925No Hit
GTCACGCATCTGATGAAGCGAATTCAGAGAGGCCCAGTAAGAGGTATCTCCATCAAGCTGCAGGAGGAGGAGAG401340.1024735852975657No Hit
GTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATA400980.10238166699710445No Hit
CATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCT396700.10128886053606498No Hit
GAGGTGAAGGATGTCAAAGAGATAAATCAAATCTACTTTCGGATTAGCCC395150.10089310118685675No Hit
TATAAATTAGCCTAGTAACTTCCTTCCTGCCCCGACCTAGACGCACTGTAAGTTGTCAGGGTCAATTTAGACACC394530.10073479744717347No Hit
ACGGTTGGAAAAAAAGATTTACACAAGCAAAAGGAAGGCTGATCGGAAAAGTATAAGGGGCGACGATTTAAACCC394050.10061223971322512No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTCCCTC391750.1000249839047226No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)