FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005284610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45796864
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACT7794911.7020619577794671No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGT7019601.5327687066083826No Hit
GTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGT5885451.2851207453855356No Hit
GGCTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTT5596571.2220421904871042No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGC5308271.1590902818149296No Hit
GTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCC5161981.1271470465750668No Hit
GGGCTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCT4911611.0724773643889678No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTC3769490.823089109332901No Hit
GGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGAC3592760.7844991307701767No Hit
GTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATT3105390.6780791802687625No Hit
GGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTG2967880.6480531068677541No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTCTAATACTGGTG2691060.5876079200532158No Hit
CACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACA2617860.5716242928773463No Hit
GCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG2449160.5347877094815924No Hit
GTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTT2413510.527003333678044No Hit
CCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGCTTGTTAGGTACTGTTTGCATTAATAA2252050.4917476445548761No Hit
GCTCCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGT2036240.44462433060918755No Hit
CTCCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA1906790.41635820304202487No Hit
AGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGAC1900360.4149541767750735No Hit
CTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGG1844850.40283325950003906No Hit
GTTTGGTAGTTTAGGACCTGTGGGCTTGTTAGGTACTGTTTGCATTAATA1805590.39426062011582275No Hit
CTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCT1802040.39348545786890554No Hit
GGACCTGTGGGCTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTT1788200.3904634168837412No Hit
GTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGG1759480.38419224512840006No Hit
GGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTC1739490.3798273174337876No Hit
GTGGGCTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGT1726380.37696467600925687No Hit
GTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCA1554190.3393660317003365No Hit
GGTAGTTTAGGACCTGTGGGCTTGTTAGGTACTGTTTGCATTAATAAATT1548610.33814760766152024No Hit
CTGTGGGCTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGG1349980.2947756422797858No Hit
CTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATG1313260.2867576260243496No Hit
GCTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTC1306660.28531647931177123No Hit
CTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG1272930.2779513461882455No Hit
AGGACCTGTGGGCTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCC1182150.2581290282234172No Hit
CGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATT1149910.25108924488803425No Hit
GGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAAT1114690.2433987619763659No Hit
CTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTA1096560.23943997562802552No Hit
ATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG1061770.23184338560823728No Hit
CTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTG1026560.22415508625219402No Hit
GCAGGTTTGGTAGTTTAGGACCTGTGGGCTTGTTAGGTACTGTTTGCATT1011080.22077494214451016No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC1009010.22032294613011055No Hit
CCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGCTT988720.21589251176674457No Hit
CGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGG984960.2150714948516999No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG923680.20169066598097196No Hit
GTAGTTTAGGACCTGTGGGCTTGTTAGGTACTGTTTGCATTAATAAATTA907380.19813147031202835No Hit
ACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCCCTAATTAAGGAACAAGTGATT829160.18105169821234923No Hit
TCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCAC814680.17788990966717722No Hit
GTCACTGGGCAGGCGGTGCCTCTAATACTGGTGATGCTAGAGGTGATGTT813800.17769775677216676No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA795720.173749888201952No Hit
GCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTTATG795270.17365162819882166No Hit
GGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTT767680.16762719822911892No Hit
CCCAGATTTATTGAAAATAATACAGCACTACAGAAAAAATTCAAACAGGT757200.16533883193399443No Hit
GTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG745120.16270109673885094No Hit
CCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATA744650.16259846962447036No Hit
CATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG743560.1623604620613324No Hit
TTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGA734070.16028826777309468No Hit
CGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCA728820.1591419010699073No Hit
GTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGACTGGTGAAG728750.15912661618053148No Hit
GTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTG721950.1576417983554507No Hit
AGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTT717770.15672907210415107No Hit
GATCAATTGAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTA714820.15608492319474102No Hit
GCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAAT689270.15050593857256253No Hit
CATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTTATGC672490.1468419322336132No Hit
CCTCGTGGAGCCATTCATACAGGTCCCTAATTAAGGAACAAGTGATTATG654270.14286349388464678No Hit
GGTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCT639320.13959907822509418No Hit
CTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGA635800.13883046664505239No Hit
GGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCCCTAATT635070.1386710670844187No Hit
ACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGC626640.13683032969244358No Hit
CCCTCGTGGAGCCATTCATACAGGTCCCTAATTAAGGAACAAGTGATTAT622630.13595472388677093No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTAT621620.13573418476863394No Hit
GGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCCCTAATTAAGGA610680.13334537491475398No Hit
GGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGAC572920.12510026887430545No Hit
CCTGTGGGCTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAG569050.12425523284738448No Hit
GTTTAGGACCTGTGGGCTTGTTAGGTACTGTTTGCATTAATAAATTAAAG568370.12410675106487641No Hit
GGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCG567020.12381197105548536No Hit
GCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAG534020.11660623749259338No Hit
GGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGT527350.11514980589063914No Hit
CCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCAC517950.11309726360302749No Hit
GTGTCACTGGGCAGGCGGTGCCTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTA505300.11033506573725221No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGC504620.11018658395474416No Hit
CGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTGCTCGG499650.10910135680906011No Hit
GACCTGTGGGCTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCAT492440.10752701320334947No Hit
GCCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGCTTGTTAGGTACTGTTTGCATTAATA484560.10580637137075587No Hit
GCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCG477000.10415560331816606No Hit
GTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGA475850.1039044944212774No Hit
GTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA473590.10341101085000057No Hit
CCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGCTTG465080.10155280501302447No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)