FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005284612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46996045
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGT7669411.6319266865967126No Hit
CTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACT5472471.164453306655911No Hit
GGGCTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCT5224141.1116126899614638No Hit
GGCTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTT4852481.0325294394453832No Hit
GTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGT4211770.8961966905938574No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGC4162610.8857362358896371No Hit
GGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGAC4057320.8633322229562084No Hit
GTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCC3863410.8220713040852694No Hit
GCTCCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGT3626490.7716585512674524No Hit
GGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTC3557600.7569998709465872No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTCTAATACTGGTG3040190.6469033724008052No Hit
GTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGG2886340.6141665750809456No Hit
GTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATT2829170.6020017216342354No Hit
CTCCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA2809870.5978949930786729No Hit
CACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACA2731820.5812872125728878No Hit
GTTTGGTAGTTTAGGACCTGTGGGCTTGTTAGGTACTGTTTGCATTAATA2718530.5784593150338502No Hit
AGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGAC2528480.5380197418740237No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTC2441430.5194969066013959No Hit
GGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTG2292130.4877282758581068No Hit
GTGGGCTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGT2267510.4824895371514773No Hit
CGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATT2184180.46475825784914454No Hit
GTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTT2167720.46125583546445237No Hit
GGTAGTTTAGGACCTGTGGGCTTGTTAGGTACTGTTTGCATTAATAAATT2052370.4367112168694195No Hit
GCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG1798730.38274071786253505No Hit
CTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGG1714420.36480091037447937No Hit
GGACCTGTGGGCTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTT1704850.3627645688057367No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG1569980.33406640920528524No Hit
GCAGGTTTGGTAGTTTAGGACCTGTGGGCTTGTTAGGTACTGTTTGCATT1527950.32512310344413026No Hit
CCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGCTTGTTAGGTACTGTTTGCATTAATAA1425110.30324041097500865No Hit
CTGTGGGCTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGG1422310.3026446161586576No Hit
ATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG1303330.2773275921409983No Hit
CTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG1253610.26674797847350773No Hit
CCCTCGTGGAGCCATTCATACAGGTCCCTAATTAAGGAACAAGTGATTAT1238030.26343280588824014No Hit
GCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTTATG1208230.25709184677136127No Hit
AGGACCTGTGGGCTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCC1203530.2560917626153435No Hit
GGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAAT1190770.2533766405236866No Hit
CTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATG1170770.24912096326403635No Hit
GTAGTTTAGGACCTGTGGGCTTGTTAGGTACTGTTTGCATTAATAAATTA1145580.24376093775550686No Hit
GTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCA1128550.2401372285689147No Hit
GCTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTC1087550.23141309018663167No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA1031740.21953762279357764No Hit
GTTTAGGGCCCTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGGGAATGTTCGCGAATAT1030460.21926525944896003No Hit
GGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCG1026400.21840135696525104No Hit
CCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGCTT1003800.21359244166184624No Hit
CATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG956780.20358734442440848No Hit
CTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTG953590.20290856390149425No Hit
GTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGTTTCGTTTCCCCGGGACCTCTTGCCCC950260.20219999363776248No Hit
CCTCGTGGAGCCATTCATACAGGTCCCTAATTAAGGAACAAGTGATTATG925100.19684635164512249No Hit
GTCACTGGGCAGGCGGTGCCTCTAATACTGGTGATGCTAGAGGTGATGTT917640.19525898402727293No Hit
CTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCT915730.19485256684897634No Hit
CGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGG886910.18872013591782033No Hit
CTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTA810300.17241876417473No Hit
GGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGAC807100.17173785581318599No Hit
AGCTCCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGG795000.16916317107109757No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC783390.1666927504218706No Hit
GGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTT777200.16537561831000885No Hit
CAGGTTTGGTAGTTTAGGACCTGTGGGCTTGTTAGGTACTGTTTGCATTA771130.16408402026170502No Hit
GACCTGTGGGCTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCAT758810.16146252306976044No Hit
TGCACTACAGAAAAAATTCAAACAGGTCCCCGAGGCGTTTTGAAATTCATCCCAACTGTAGGCTGAGTGACCTGA737120.15684724108166975No Hit
CGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCA717070.15258092462887035No Hit
ACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGC714690.152074499034972No Hit
ACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCCCTAATTAAGGAACAAGTGATT710370.15115527274688753No Hit
CCTGTGGGCTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAG694450.14776775364820594No Hit
GGGCAGGATGGCAGGAAGAACCTCATACCCAAGCAGAGTGCCAGGTTTTA674450.14351207638855568No Hit
GCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAG671560.14289713102453622No Hit
CTCGTGGAGCCATTCATACAGGTCCCTAATTAAGGAACAAGTGATTATGC668290.1422013277925834No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA668040.14214813182683778No Hit
GGTTTGGTAGTTTAGGACCTGTGGGCTTGTTAGGTACTGTTTGCATTAAT663180.14111400225274276No Hit
GCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAAT654260.13921597019493875No Hit
GGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCCCTAATT650660.1384499482882017No Hit
GTGTCACTGGGCAGGCGGTGCCTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTA625280.13304949384570552No Hit
AGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTT620930.1321238840417316No Hit
GTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG609730.12974070477632743No Hit
TGTATATTAAGGTTGGTTGCGTCATTACAGGAACACTTCCCATTTGTGGA602560.12821504447874282No Hit
CATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTTATGC600640.1278064994618164No Hit
GATCAATTGAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTA596050.12682982153072667No Hit
CCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATA588850.1252977777172526No Hit
CCTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGG584530.12437855142916814No Hit
GCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCG580450.12351039326819947No Hit
GACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCCCTAATTAAGGAAC565950.12042502725495305No Hit
TGGTAGTTTAGGACCTGTGGGCTTGTTAGGTACTGTTTGCATTAATAAAT560700.11930791197429486No Hit
CTCAAGAACTCGGGAACAATTAGCGCCAAAGAAACACAGACCAGGATAAG556680.11845252084510516No Hit
GAGGAATTAACAGTCTTTATTGGGCTCAGACCAGGAGTCCGTGGGTCTTG549160.11685238619547665No Hit
CTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTTTCTCTTGCGTCT525530.1118243035131999No Hit
GTTTAGGACCTGTGGGCTTGTTAGGTACTGTTTGCATTAATAAATTAAAG523720.11143916472120155No Hit
TCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCAC522430.11116467353795409No Hit
CCTGGGGTCAGTAACCACAAGAAGCCGTGGCTCCCGGAAGGCTGCCTGGA518440.11031566592465387No Hit
CCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCAC517490.11011352125482048No Hit
GGCCAGTTGGGCAGCTCTTTCCACGATGGCTTTGCGGTTCTTGGAGGAAACATTGTGAGCGATCTCGGCACAGTA516420.1098858425214292No Hit
ATCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGA502490.1069217633100828No Hit
AGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCCCTAATTAAGGAA501390.10668770106080203No Hit
CAACCGCTATCACGATTGATAGACTTTACATTCGCGAGAGCTACTGATTC500160.10642597690933353No Hit
GTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGACTGGTGAAG497870.10593870186310358No Hit
CAGCTGAACCCTCGTGGAGCCATTCATACAGGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCACGGTT497070.10576847477271757No Hit
CCCTGGGGTCAGTAACCACAAGAAGCCGTGGCTCCCGGAAGGCTGCCTGG487160.10365978669056088No Hit
GGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGT475380.10115319278462688No Hit
ATGCAGGTTTGGTAGTTTAGGACCTGTGGGCTTGTTAGGTACTGTTTGCA472120.10045951739130388No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)