FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005284615

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27114316
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTT8892033.279459456030534No Hit
CGTATATAAATTAGCCTAGTAACTTCCTTCCTGCCCCGACCTAGACGCAC2495810.9204768433029991No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA1995060.7357958061711755No Hit
AAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCC1463340.5396927586150431No Hit
ATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGT1448920.5343745348398241No Hit
TGTAAGTTGTCAGGGTCAATTTAGACACCACTCCCCTTATACCCCACCTC1402160.5171290325007645No Hit
ATTTAGACACCACTCCCCTTATACCCCACCTCCCTTCACCTCCCTATGAT1159910.4277850859302518No Hit
AGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCT1105300.40764443403256045No Hit
CACCACTCCCCTTATACCCCACCTCCCTTCACCTCCCTATGATTCGCCCTTTCCCGTCCACAGAGACACGGCTTC988780.364670825552081No Hit
GTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGT942890.3477461869220673No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG859030.31681787584093957No Hit
GTTTCTCTCACCCTTGCATCCTCATTACTCTCTTAGACGCTTGAATCATG768350.2833742883279814No Hit
GAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTT708690.2613711516823806No Hit
AAATTAGCCTAGTAACTTCCTTCCTGCCCCGACCTAGACGCACTGTAAGT693550.2557873855272617No Hit
ATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTTAATTCCCCTATGG693380.25572468802089643No Hit
ATTCAAGAGAAGGTAAAGAACACTTGCACACATGTGCTAATGTTTCTCTCACCCTTGCATCCTCATTACTCTCTT693130.2556324858056534No Hit
ATAAATTTTACCATATCGGATCATCGAATAGAAGCCGTGACGTATATAAA663620.24474893631836409No Hit
ATCGTGAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAG660760.2436941429759836No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCA625620.23073419960142086No Hit
GATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCC621680.22928109268919045No Hit
AAAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTTA618850.2282373636126392No Hit
GCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGG600600.22150660189989674No Hit
GTATATAAATTAGCCTAGTAACTTCCTTCCTGCCCCGACCTAGACGCACTGTAAGTTGTCAGGGTCAATTTAGAC593210.21878110441731227No Hit
ATTTGAAAATCATCACCACCATCCTTAGGAATTTATTCAAGAGAAGGTAA574940.21204296652735036No Hit
CGATGAAGAACGCAGCTAGCTGCGAGAATTAATGTGAATTGCAGGACACA545080.20103033393872077No Hit
ATCATCGAATAGAAGCCGTGACGTATATAAATTAGCCTAGTAACTTCCTTCCTGCCCCGACCTAGACGCACTGTA541410.19967680541895286No Hit
GTGATTTTTTGAGCTGTGGTCTGTACACTTTATTGTTGTCCATCGTCTGT537820.1983527816080627No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT532210.1962837638980087No Hit
AGAATTTCTTATTTTTTAGTTAAGTGTATTAAATTTGAAAATCATCACCACCATCCTTAGGAATTTATTCAAG507060.18700822104455816No Hit
CCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTTAATTCCCCTATGGTCCTACTTTACAGTCTGAAT487330.17973162221757685No Hit
TGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTG482580.17797978012795895No Hit
GTTAAAATTAGCTTTATATCAGAATTTCTTATTTTTTAGTTAAGTGTATT480420.17718315298825904No Hit
GGGGAGCTCTACAAAATGTGCTGCTTGTCAGAATCCGAGAAAACAGAGTC476630.17578536740517445No Hit
CAGCAATTGAAGTGAAGATGATTGACCGCACGCAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAG471160.17376798293565657No Hit
CATATATTCGCGAACATTCCCAAGTTGGCAAAAGAGTCCCATGGTTTTCTTTCTCTCGCACGATCCAGGGCCCTA470360.1734729358468788No Hit
GGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGCTTGAAGC469510.17315944831505245No Hit
AGATGATTGACCGCACGCAATTGATATTAGATATTTTTGCAAAGCGGGCG459490.16946398352811115No Hit
TAGGAATTTATTCAAGAGAAGGTAAAGAACACTTGCACACATGTGCTAAT458030.16892552259109173No Hit
ATTAGCCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTTAAT453440.16723268991922938No Hit
CATCATTATTGCTTCATTTCCCAATTAACATACTCTCTACCCTGCCGAATCTTCGTTATTCTATAACTATATGT451750.16660940294418639No Hit
CTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCAC445990.16448506390498657No Hit
GGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGT435570.16064207557365637No Hit
GGGGGAAGTAGTTTGATTGTTCGGATAATTATGTTTCACTATGCTCCTCC421400.15541605401368044No Hit
CCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGAC420690.1551541997223902No Hit
CTCAGATATCTTACAAGAGGATAATTAAAAGCAAGTATATTTGAATTACA417010.15379698311401255No Hit
AATTGGGGCAAGAGGTCCCGGGGAAACGAAACTGGAAACCGACCGCCGCC416060.15344661469608895No Hit
GTAAGTTGTCAGGGTCAATTTAGACACCACTCCCCTTATACCCCACCTCC410370.1513480922771572No Hit
CAATAACGTCTCTTTCAGAAGGCATTGGTATCTTTTCCCCACTTCCAAGC409470.15101616430228224No Hit
CACGATTATGTTGCGCACTCGTCGTGCTAGCCCCCCCGTCGTCCTTGCCA404230.14908360587078798No Hit
GGGGAAACGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGCATTCA398260.146881816970784No Hit
GTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAG395480.1458565283372813No Hit
GTCCACACTTCCATAGTTTAATGCATTCATAAAGCCCCAGCCCCTGATCA391250.14429646685536895No Hit
GTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTTACCA390900.14416738375402868No Hit
GGAGAGTGTGTGGGCTGGACCAACGGAGGTGTTTAAGATACACAATCTCT388330.14321954498133016No Hit
CAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAG387120.14277328625955382No Hit
CGAATAGAAGCCGTGACGTATATAAATTAGCCTAGTAACTTCCTTCCTGC385230.14207623751231638No Hit
GTTATTAACATTAATTATTGTCTTATTCTATTCTCTTGGATGAATATTAA384790.1419139616134886No Hit
AAGCTAAGCTCGATCGCCCAAAGCGGCGGCTAACCATAACTACGGATCCC379470.13995189847311656No Hit
GATTAATTCTGTGCTACTCTTCGCTTCCTCGAACACCACTGTTTGTGTTT373950.13791607356055008No Hit
CGGGTCCTTACATCTAATCTCCAGACAGCGTGTGCACACAAATGGACGCG369720.13635601207863773No Hit
CTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAATGTG368290.1358286154074475No Hit
GGGATTCGCCTATATACTGAATACCCACCATGCCTGTATGAGCCTACGAA367700.1356110181794739No Hit
GGTGAATCCACACACGGGATCTAAAGCCGGTGTTGAGAAGAAAAACATAC366010.1349877312044309No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACAC365990.13498035502721145No Hit
GCTTTATATCAGAATTTCTTATTTTTTTAGTTAAGTGTATTAAATTTGAA365210.13469268411565313No Hit
AGATGTTAAAAGGAGCAGAAATAGCGATATAAAAGCATTCTTCACTGGCA362810.1338075428493199No Hit
AGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTC362450.1336747716593699No Hit
TAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCAT359670.13264948302586724No Hit
AGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCACAC358600.13225485754462699No Hit
ACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTT353610.13041450132837576No Hit
ATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGCAAGGC352820.13012314232820774No Hit
CTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTT349940.12906097280860782No Hit
CCACTCCCCTTATACCCCACCTCCCTTCACCTCCCTATGATTCGCCCTTT347920.12831597890944402No Hit
GCTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCA341630.12599617117392892No Hit
GTACACTTTATTGTTGTCCATCGTCTGTATAATTAAAGGGTGCTGTGTCT337980.12465001883138044No Hit
GGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACC336410.12407098891965411No Hit
GTTGTTATGAAAACAGATGCTGAGTTTGTGTGTGAACGGACACTGAAATA336360.12405254847660549No Hit
GTAAAGAACACTTGCACACATGTGCTAATGTTTCTCTCACCCTTGCATCC336330.12404148421077633No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT335790.12384232742585134No Hit
CTCGAACACCACTGTTTGTGTTTGATTGTCTGTCTTTCGTTTTTTCCTTCTCTTCTTACCCCCATCCGGGTCCTT334270.12328173795717362No Hit
GTTTCTCTGAACGTGTAGAACACCGAAAACCACGAGGAAGAGAGGTAGCG333540.12301250748866394No Hit
GTTGGGTATGCTGTTCGATTTCTTAAAGGACTGAATATTCGGTGGCAGTATGGGATTTCTAAAAATAATGTTA333280.12291661718481116No Hit
CGGGGAAACGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGCATTC329950.12168848367777377No Hit
CCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTG327840.12091029698112245No Hit
CATCACACTACTAAGTATTTTTATATGCTATATGGTCACTCTCTGTTATG325290.11996983438564338No Hit
AAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGTTTC323960.11947931860055035No Hit
ATCAACCTTTCCCGGCAAGGCGGAGGAATTGGGGCAAGAGGTCCCGGGGA322650.11899617899267678No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT320590.11823643273907408No Hit
GGTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGA320330.1181405424352213No Hit
GAACGCAGCTAGCTGCGAGAATTAATGTGAATTGCAGGACACATTGATCA319050.1176684670931769No Hit
CTAAACCAAATCATCACACTACTAAGTATTTTTATATGCTATATGGTCAC318000.11728121778915611No Hit
CCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACT317050.11693084937123253No Hit
CCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCA316070.11656941668747978No Hit
AGAGAAGGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCACTGCC314520.11599776295297289No Hit
CAGCGGGATGGGGAGGACCAGACCCAGGACACGGAGCTCGTGGAGACCAGGCCTGCAGGGGATGGAACCTTCC314230.11589080838329095No Hit
CATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATG313070.1154629901045632No Hit
AGAACGAGAGAAGGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCACTGCCGCGTCTGACGGGACAAGGG312320.11518638345883406No Hit
AGTGAATCCGCTACTGTTCTCGTAGTCGTTCTTTGCCTGGGCTGGCTCCG311280.114802822243423No Hit
CGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGGTCAGTAATTCCTGGAGGAATAGGTACCAAGAAAAA309260.11405782834425918No Hit
GGGTGCTGTGTCTTGATTTAGGATCTGTTATGTTATGTGTGTTACTTTTATGAAGTTAAAATTAGCTTTATATC309160.11402094745816195No Hit
CTAATCTCCAGACAGCGTGTGCACACAAATGGACGCGTTGCGATGGGGAG308530.11378859787574946No Hit
GCAAGAGGTCCCGGGGAAACGAAACTGGAAACCGACCGCCGCCATATCAG308290.11370008374911615No Hit
CAGCCAAAGAGGTCGAAGTGGTTCTGGAAACTTTGGTGGTGGTCGTGGAG306040.11287026381192873No Hit
AGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAG304110.11215846271025239No Hit
GGGGACTCCGATTATCACGTCTTGATTTTGAAGAGAGACAAGGGAAAAAGAAGAGCTGTTTTGTTGTGGGTAACA303060.1117712134062316No Hit
AAGGTTTTCTCAAACTTCACTAGTGCTGTGTTATTAACATTAATTATTGT302070.11140609263386914No Hit
CGTCGAATGAGGCACGGAGAAAAATGGGAGGAGCAGAAGTGAAAGAGAAA301960.1113655236591622No Hit
AGGATCTGTTATGTTATGTGTGTTACTTTTATGAAGTTAAAATTAGCTTT301810.11131020233001636No Hit
ACGAGAGAAGGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCACTGCCGCGTCTGACGGGACAAGGGATC301170.11107416465899417No Hit
GCCCCGTCAAGCACCGAAATAATATCGTTAGCGCCTCGATCCTGGAGAAT301070.11103728377289694No Hit
GGGGCAAGAGGTCCCGGGGAAACGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGCATTCATGAAATCAAC297550.10973907658227484No Hit
ACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTAGTACTTGGATGGG293720.10832653864475135No Hit
GGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACATTCA291680.10757416856836809No Hit
GAAGAACGCAGCTAGCTGCGAGAATTAATGTGAATTGCAGGACACATTGA291130.10737132369483338No Hit
CTTCTTCATGCCCGGCTTCGCGCCCCTGACCAGCCGGGGCAGCCAGCAGT289850.10689924835278898No Hit
AGCTGAAACTATAATGGCTTCAAGCAACGAATCCTTAGTTGTCAATAGGG288600.10643823727657375No Hit
AATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATG287280.10595140958009047No Hit
AGGACTATAGAGGACACCGTCACAGTGCATTTGAGGTAAGAGCAATCCAGAACAGAGATTAATGAAGTCAAAAAC286900.10581126221292103No Hit
GATATCTCCACTTAGCTGGTAATAAGCTAGTCTACAAGCAATGCTGTCTG286360.10561210542799604No Hit
GCCTGCAATGCCCGGTACTAACAGGGCCGGGCACTGCTACACCAGGCGGGTCCGCTTCCCCAGAGCCTTAGAAGC285870.10543138908611968No Hit
CACTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAATG283470.10454624781978641No Hit
TTGCTGTTTACCATTGGTCTGTTTTAGGTAGTATCTTCCATTCAGTATTTTATTCGGAAGATGTTAAAAGGAGC283350.10450199075646975No Hit
AGAGCTTGAAGCTGATCTCCTCATCTTTAATGATGAACTGTCGCCAAGTC283090.10440610045261697No Hit
GACACCACTCCCCTTATACCCCACCTCCCTTCACCTCCCTATGATTCGCC282820.1043065220601545No Hit
GAGAAAAAGTACAACGGAGAAAGAGAAAGGCAAACTACCGCAGTGACCGC282180.10407048438913229No Hit
CGAATAGCTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAG281150.10369061126233094No Hit
CCATGGTTTCCTCCCTTCCTTAATTCCCCTATGGTCCTACTTTACAGTCT280640.10350251874323513No Hit
CTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATAT279860.10321484783167682No Hit
CCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTC278260.1026247536541213No Hit
CAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGG277500.10234445891978246No Hit
TGATTGGAACGAGATAATCACACCCGTCTGATTCACAGAGTGTCTCCCAA277000.10216005448929635No Hit
AGATGTGTAAAACTAGAATCCGTCACGTGGTACCTGTAGTTCACGTTACT276440.10195352152715194No Hit
ATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGTTTCCT273340.10081021405813814No Hit
AGCCGACGCCCAGAAGGGGGATGCTACCCGGAGCACCAGTATGCGGCCGAGCGCGCTGGAGCAGGCCACGCGATA272730.10058524065294512No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)