FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005284620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8209999
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA129974715.831268676159398No Hit
TCTTAACCCGGACCAGAAACTATGGAATTCTCGGGTGCCAAGGAACTCCA6247877.610074008535202RNA PCR Primer, Index 1 (100% over 28bp)
AGGTTCCGGATAAGTAAGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCA4892005.958587814687919RNA PCR Primer, Index 1 (100% over 28bp)
GTCCCACTCCGTAGATCTGTTCTGGAATTCTCGGGTGCCAAGGAACTCCA3134833.8183074078328145RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA1397701.7024362609544776No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGATCTCGTATGC1365621.6633619565605309RNA PCR Primer, Index 17 (100% over 50bp)
CCTGGAACTTAGGACGTGAATCTGGAATTCTCGGGTGCCAAGGAACTCCA445790.5429842317885788RNA PCR Primer, Index 1 (100% over 28bp)
TAGCATATCGAGCCTGAGAACATGGAATTCTCGGGTGCCAAGGAACTCCA425730.5185506112729125RNA PCR Primer, Index 1 (100% over 28bp)
TGATACGGATGTTATACGCAGCTGGAATTCTCGGGTGCCAAGGAACTCCA397530.4842022514253656RNA PCR Primer, Index 1 (100% over 28bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG353250.43026801830304734No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG321140.39115717310075193No Hit
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA293960.35805120073705243RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGATGTCGTATGC285550.3478075941300358RNA PCR Primer, Index 17 (98% over 50bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT260510.3173081994285261No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC257800.31400734640771577No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTTGGAATTCTCGGG219670.26756397899683054No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTA180830.22025581245503195RNA PCR Primer, Index 3 (97% over 36bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG169290.2061997815103266RNA PCR Primer, Index 6 (100% over 34bp)
TGTAGTATTCTCTGATGGTAGTTTGTATTTCTGTGGAATTCTCGGGTGCC157870.19228991379901508No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGAATTCTCGGGTGCC157870.19228991379901508No Hit
ATAGGCGATAGAAATTGAAACCTTGGAATTCTCGGGTGCCAAGGAACTCC152060.18521317724886446RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG148860.18131549102502936RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC146830.17884289632678396No Hit
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC120430.14668698498014435RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGAGCTCGTATGC119250.14524971318510516RNA PCR Primer, Index 17 (98% over 50bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACATGGAATTCTCGGGTGCCA110120.1341291271777256No Hit
AGATTTCAACTTAACTTGACCGCTCTGACCATGGAATTCTCGGGTGCCAA109020.13278929753828228No Hit
TAGGATGGGTGTGATAGGTGGCACGGAGAATTTGGAATTCTCGGGTGCCA95260.11602924677579132No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGAGGTCGTATGC92010.1120706592047088RNA PCR Primer, Index 17 (97% over 40bp)
AGGTTCAGGATAAGTAAGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCA91020.11086481252920982RNA PCR Primer, Index 1 (100% over 28bp)
CCCGTGAAAGTAGGACATCGTCAGACATGGAATTCTCGGGTGCCAAGGAA90750.11053594525407373RNA PCR Primer, Index 1 (100% over 23bp)
GCTCTACACTCTACTCGTCAGCTGTTGGAATTCTCGGGTGCCAAGGAACT87330.10637029310234995RNA PCR Primer, Index 1 (100% over 25bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAATGGAATTCTCG87080.10606578636611283No Hit
TTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCC84040.10236298445346949No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)