FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005284623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3008785
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA2106367.000699617952097No Hit
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA970333.224989489112715RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG529671.7604115947134809RNA PCR Primer, Index 1 (100% over 29bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC453701.5079176478212968RNA PCR Primer, Index 2 (100% over 34bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC405711.348418049146084RNA PCR Primer, Index 1 (100% over 27bp)
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA403151.3399096312963539RNA PCR Primer, Index 1 (100% over 23bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA316391.0515540326078467No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC208570.6932034027024198No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA186150.6186882745028308RNA PCR Primer, Index 1 (100% over 28bp)
AGGGCTGGGTCGGTCGGGCTGGGGCTGGAATTCTCGGGTGCCAAGGAACT110290.3665599236901274RNA PCR Primer, Index 1 (100% over 25bp)
AAACCGTTACCATTACTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG98250.3265437709906158RNA PCR Primer, Index 1 (100% over 29bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG78750.26173355690087524RNA PCR Primer, Index 2 (100% over 35bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGT77560.2577784720410398RNA PCR Primer, Index 12 (97% over 36bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTCGTATGC75690.2515633386898698RNA PCR Primer, Index 22 (100% over 50bp)
CCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAAC75330.25036684242975155RNA PCR Primer, Index 1 (100% over 24bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG74370.24717618573610278Illumina Small RNA Adapter 2 (100% over 21bp)
TCTTAACCCGGACCAGAAACTATGGAATTCTCGGGTGCCAAGGAACTCCA72220.24003044418261857RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC69360.23052494611612329No Hit
GCTCTACACTCTACTCGTCAGCTGTTGGAATTCTCGGGTGCCAAGGAACT60780.20200845191663744RNA PCR Primer, Index 1 (100% over 25bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA60520.20114431572877423RNA PCR Primer, Index 1 (100% over 28bp)
AGGTTCCGGATAAGTAAGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCA57290.19040908539493515RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC55820.1855233923327855RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT53180.1767490864252514RNA PCR Primer, Index 1 (100% over 30bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACATGGAATTCTCGGGTGCCA52480.17442256591946584No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG44240.14703609596564726No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGGAATTCTCGGGTGC42730.14201745887459555No Hit
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG41360.13746412588470097RNA PCR Primer, Index 1 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGAATTCTCGGGTGCC40990.13623439361735717No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTTGGAATTCTCGGGTGCCAAGGA40910.1359685055595531RNA PCR Primer, Index 1 (100% over 22bp)
GTCCCACTCCGTAGATCTGTTCTGGAATTCTCGGGTGCCAAGGAACTCCA35190.11695750942656254RNA PCR Primer, Index 1 (100% over 28bp)
CCCCCCACTGCTAAATTTGACTGGCTATGGAATTCTCGGGTGCCAAGGAA34260.1138665607545903RNA PCR Primer, Index 1 (100% over 23bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA32890.10931322776469572RNA PCR Primer, Index 1 (100% over 32bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC32230.10711965128781219RNA PCR Primer, Index 1 (100% over 33bp)
CAACTCTTAACAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTA30680.10196807016785846RNA PCR Primer, Index 22 (100% over 37bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTAGC150.002224379570.006954
GGCTGGT274950.069.5474551
GCTGGTC275400.069.523972
TGGTCCG285250.069.4670264
TGCCCCG238550.069.1748914
GGTCCGA288500.069.073865
AGTGGGT273350.069.02112616
CTGGTCC277800.068.973733
TTATCAG272250.068.9412122
GCCCCGA233350.068.89931515
GTCCGAT273800.068.885366
GAGCTGC240900.068.880110
ACTGAGC231750.068.833647
TATCAGA273000.068.8287323
GGGTTAT282200.068.778819
TCCGATG274150.068.758177
GCTGCCC240450.068.7470112
TAGTGGG274450.068.74448415
CTGCCCC240950.068.7037413
ATCAGAA274400.068.6700124
Produced by FastQC (version 0.11.5)