FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005284624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35674831
Sequences flagged as poor quality0
Sequence length35-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTTTCTCTTGCGTCT2764910.7750310015484025No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2725490.7639811944729324No Hit
GCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTT2186360.6128578436713548No Hit
GGCAATGCTTGTGGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTA1395870.3912758549577992No Hit
CCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTTT1381010.38711045330530086No Hit
CTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACT1378660.38645172558771196No Hit
GTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGC1332990.3736499830931224No Hit
GTTCAATCGTTTCTTTGGAAGGCAGTGGATTTTTCTCTTGCGTCTCTGTC1064910.2985045675479163No Hit
GCCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGG1028510.2883012956669648No Hit
GTTTCTTTGGAAGGCAGTGGATTTTTCTCTTGCGTCTCTGTCTTCTTCAG909940.25506497844376613No Hit
GTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCC869470.24372084621788395No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGT767110.2150283486977135No Hit
GTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGT725600.2033926944180899No Hit
GTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATT713530.20000935673668646No Hit
GGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGAC711350.19939828166249757No Hit
GGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGC694760.19474794428598693No Hit
CGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTTTCTCTTGCGTCTCTGTCTTCTTC671700.18828400336360387No Hit
CTCCACTTTTTCAATTCTCTCTCCATTCTTCAGTAAGTCAACTTCAATGTCGGATGGATGAAACCCAGACACAT639670.17930568472770061No Hit
GCTTGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTTTCTC620000.17379199357664793No Hit
GTCATTTTTGTCAGTGGCCAGTTTGTGCAGTTCCAGTAGTGACTGATTCA611600.1714373923733514No Hit
CTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTTTC611010.17127200966978653No Hit
GGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATC603170.16907438188004312No Hit
CTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGAA599220.16796715869515963No Hit
GTGGATTTTTCTCTTGCGTCTCTGTCTTCTTCAGTTTCGACTTATCGAAT590940.1656461946519102No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGC576500.16159852305957664No Hit
GCTTCTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTTTCTCTTGC561220.15731539134691347No Hit
CTCCATTGCATTCAGCCCGCTCTCCCAGTCATCACAGTCTGGTTTCTTGA556420.1559699049450297No Hit
CTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTG479660.1344533349015725No Hit
GTCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTT468550.1313390945005458No Hit
CTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAAAAATCCACTGCCTTCCAA467100.13093264548331007No Hit
AGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGC447400.1254105450422456No Hit
CATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGAAGG435130.12197114542743033No Hit
CTTGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTTTCTCTTGCGTCTCTGTCTTCTTCAGTTTCG421740.11821779898550885No Hit
GTGGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCT402390.1127938069279151No Hit
AGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTTTCTCTTGCGTCTCTGTCTTCTTC394590.11060739152485403No Hit
ATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGC389090.10906568835602894No Hit
AGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGAC388440.1088834870724405No Hit
GTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGT380270.10659335709256759No Hit
GGCCAGTTTGTGCAGTTCCAGTAGTGACTGATTCACATTTTTTTCCAAAT373350.10465361419651856No Hit
CCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTTTCTCTTGCGTCTCTGTCTTCTTCAGTTTCGACTTATCGA371830.10422754350258871No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTC367780.1030922893509993No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)