FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005284625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35674831
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAAAAATCCACTGCCTTCCAA5207861.4598135026904542No Hit
GGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAAAAATCCACTGCCTTCCAA2361820.6620409778535461No Hit
GTCTGACAAACCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAA2083960.5841541337645019No Hit
CTGACAAACCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAAAA2029390.5688576352330863No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA1698160.47601066421309746No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1423840.39911611634544253No Hit
GACAAACCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAA1314680.3685175130892701No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA981450.27510992273516305No Hit
AGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAAAAATCCACTGCCTTCCAAAGAAACGATTG960920.26935516527043957No Hit
GTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGT955350.2677938404249203No Hit
GAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAAAAA916670.25695146250307394No Hit
CGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAAAA908980.2547958811633894No Hit
TGACAAACCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGA847630.237598883089313No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCA804000.22536897231552408No Hit
CCTCGCTTCGCTTTTCCTCCGCAACCATGTCTGACAAACCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGA803740.22529609180208873No Hit
GCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAAAAATCCACTGCCTTCCAA790850.2216829001936968No Hit
ATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAAAAATCCACTGCCTTCCAAAGAAACGATTGAAC730520.2047718179800207No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACAC684460.1918607547152781No Hit
CAGCATTCGGGCCGAGATGTCTCGCTCCGTGGCCTTAGCTGTGCTCGCGC662160.18560984913985998No Hit
GTGGCTTTGAAGAACTTTGCCAAATACTTTCTTCACCAATCTCATGAGGAGAGGGAACATGCTGAGAAACTGATG639320.1792075763442299No Hit
CACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCA636390.17838626901974672No Hit
CTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAACCATGTCTGACAAACCCGATATGGCTG635070.17801626025922868No Hit
CTTTTCCTCCGCAACCATGTCTGACAAACCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGA615870.1726343146516938No Hit
CCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGAC600540.16833716745567764No Hit
AATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATG580820.162809460821272No Hit
ACCATGTCTGACAAACCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCA560770.15718925199673686No Hit
CCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCACGAGGGTTC518560.14535738095017184No Hit
GTCGAAACTGAAGAAGACAGAGACGCAAGAGAAAAATCCACTGCCTTCCAAAGAAACGATTGAACAGGAGAAGCA499320.1399642229559546No Hit
GGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAACCATGTCTGACAA497140.1393531478817657No Hit
CCGCAACCATGTCTGACAAACCCGATATGGCTGAGATCGAGAAATTCGAT497070.13933352620507158No Hit
GGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACC495860.13899435150793008No Hit
GACAGCATTCGGGCCGAGATGTCTCGCTCCGTGGCCTTAGCTGTGCTCGC483820.13561942311653838No Hit
ACCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGA483250.13545964660631468No Hit
CACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTT476650.13360960280372455No Hit
CATGTCTGACAAACCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAG462650.12968526746489703No Hit
GCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGG450860.12638041649027013No Hit
CCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTT434750.12186462775394787No Hit
CAAACCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAAAAATCC395090.11074754635838359No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT385300.1080033147178749No Hit
AAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAAAAATCC372480.10440974478617712No Hit
AAACCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAA362390.10158142024555071No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG55400.031.5521564
CTAGTCC61400.028.36218528
CTTATCG115700.027.6805670
AACGTCT70800.026.1069073
CCTAGTC66300.025.74348427
CGCTAGG7950.024.75601670
AGGCGTA9100.024.72518215
CTCCTAG70050.024.6121925
AGGCGGG884800.024.40716470
TCGCGAG3900.023.9632973
CGCGTTG11350.023.6815167
CATTCGG212050.023.6035564
GCATTCG214950.023.4139353
GGTACGT7800.023.30199864
CTAGACG17850.023.28461341
TAAGCTC73550.023.25126321
CGTAACG8500.023.23426445
TCCTAGT73950.023.1271526
GACGCCA77400.022.77039136
TTATCGT71300.022.66802670
Produced by FastQC (version 0.11.5)