FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005284646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31332176
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACT1161800.37080093000881903No Hit
GTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCC1073100.34249137372393157No Hit
CTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGG789810.2520763320109015No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGT620380.19800093041734476No Hit
GTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCA483050.1541705880881047No Hit
CCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA476600.152112001413499No Hit
CTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTG471920.1506183292216921No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGC427290.13637418607631976No Hit
CTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTA407960.13020480926699762No Hit
GTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGT397620.12690468737313362No Hit
GTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATT380020.12128745861762043No Hit
CTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATG365380.11661494560735264No Hit
GTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGT339330.10830080872774364No Hit
GGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGAC332720.10619115633717877No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTCTAATACTGGTG322850.10304103998394494No Hit
CACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACA321470.10260059818379674No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGCT456650.025.98141570
CTTATCG96600.020.77741270
ATTATGC456450.019.24973169
TACCGCG239700.018.75810870
GTCGCGG14400.018.3358731
GATTATG453650.018.02400668
CCGACCG19300.017.8141943
CGTTACT55800.017.1125225
CGCGAAC4750.016.85721257
TCGCCGT58100.016.85327321
CGAGGCG55000.016.38171654
CCGTTAC58400.016.29115324
CGCATTA15550.016.0861661
TACCGAC30100.016.01736670
TGATTAT541250.015.98523270
CGCGTTA5050.015.81616434
GTGATTA503500.015.73835166
TACGCCC18350.015.708892
AACCGTA20200.015.66059650
TACGTCG11000.015.47627244
Produced by FastQC (version 0.11.5)