FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005284647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31332176
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCA1046390.33396659076599083No Hit
GTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGT1038830.33155373568691815No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA1018460.3250524317238611No Hit
CAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAG952370.3039590994254596No Hit
CACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCA851900.27189302141032273No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACAC691670.2207538984844206No Hit
CCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCACGAGGGTTC597580.19072406589315724No Hit
GGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACC484450.1546174131027478No Hit
AATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATG436510.13931684795846927No Hit
CCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTG417800.13334535079848908No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG416150.13281873560265972No Hit
TGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAAT392360.12522590196097455No Hit
GTTTACCAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCT358050.11427549749497132No Hit
CCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACT348140.11111261471274769No Hit
GCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGG332370.10607945008351799No Hit
TATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAA332010.10596455222260975No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCGC36350.020.35349570
GCGTTCG20550.020.25323915
CGTTCGC21650.019.22420716
CGTCGAA46600.018.4579989
CGCGTTC24250.017.44896514
ACTGCGC60550.017.2979353
TTCGCAC26850.016.53514718
CCCGTGT82900.016.4038871
ACACGCG31200.016.39865569
CGACCCG70350.016.3674766
CGCGCGT6950.015.97231933
ACGGGTC27100.015.7416572
CGTAACC34200.015.4141283
ACCTCTA476450.015.38886211
TCGTAAC35400.015.3819392
CGTGGCG75400.015.3210371
ATTTCGG110200.015.30926570
TCGGGGT81200.015.1630642
GTTCGCA29650.015.09041417
CTCGTAA40150.015.0341541
Produced by FastQC (version 0.11.5)