FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005284650

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284650
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3054396
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA40070313.118894864974942No Hit
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG1583575.184560220744134RNA PCR Primer, Index 6 (100% over 34bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA473821.5512723301104374No Hit
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGG417901.3681919436772443RNA PCR Primer, Index 2 (97% over 35bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA256610.8401333684302887RNA PCR Primer, Index 1 (100% over 28bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGC192090.6288968424526485RNA PCR Primer, Index 6 (97% over 36bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG188890.6184201393663428RNA PCR Primer, Index 1 (100% over 29bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC160530.5255703582639579RNA PCR Primer, Index 1 (100% over 33bp)
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA160040.5239661131038673RNA PCR Primer, Index 1 (100% over 23bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA125130.4096718303717003RNA PCR Primer, Index 1 (100% over 32bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA109600.35882708070597263RNA PCR Primer, Index 1 (100% over 28bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA88010.28814207457055335RNA PCR Primer, Index 1 (100% over 22bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC81160.26571538202643014RNA PCR Primer, Index 1 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGAATTCTCGGGTGCC79770.26116456412331607No Hit
GGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCAAGGA72970.23890157006491627RNA PCR Primer, Index 1 (100% over 22bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC65600.2147724132692683RNA PCR Primer, Index 1 (100% over 27bp)
TTCGACCTGAGCTCAGGCAAGGCTACCCGTGGAATTCTCGGGTGCCAAGG65260.2136592635663483Illumina Small RNA Adapter 2 (100% over 21bp)
TGAACGCAAATTGCGCCCGAGGTGGAATTCTCGGGTGCCAAGGAACTCCA64180.2101233762747201RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGC62990.20622735231450015RNA PCR Primer, Index 11 (100% over 50bp)
AGGTTCCGGATAAGTAAGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCA60990.19967941288555904RNA PCR Primer, Index 1 (100% over 28bp)
CCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAAC58010.1899229831364368RNA PCR Primer, Index 1 (100% over 24bp)
TCTTAACCCGGACCAGAAACTATGGAATTCTCGGGTGCCAAGGAACTCCA56770.1858632606904933RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACATGGAATTCTCGGGTGCCA54010.17682710427855458No Hit
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG51600.16893683726668055Illumina Small RNA Adapter 2 (100% over 21bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA50030.16379670481496178RNA PCR Primer, Index 1 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCTGGAATTCTCGGGTGCCAAGGAA49250.16124300843767475RNA PCR Primer, Index 1 (100% over 23bp)
TTTGAACGCAAATTGCGCCCGAGGTGGAATTCTCGGGTGCCAAGGAACTC48990.1603917763119124RNA PCR Primer, Index 1 (100% over 26bp)
TTCGACCTGAGCTCAGGCAAGGCTACCCTGGAATTCTCGGGTGCCAAGGA45300.14831082806551607RNA PCR Primer, Index 1 (100% over 22bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCT44770.14657562411684666RNA PCR Primer, Index 11 (100% over 37bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC43610.14277781924806082RNA PCR Primer, Index 1 (100% over 24bp)
ATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT43020.1408461771165232RNA PCR Primer, Index 1 (100% over 30bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC39280.12860153038440333RNA PCR Primer, Index 1 (100% over 27bp)
CTTTGGACACTAGGAAAAAACCTGGAATTCTCGGGTGCCAAGGAACTCCA37900.12408345217843396RNA PCR Primer, Index 1 (100% over 28bp)
AAGATTAAGAGAACCAACACCTTGGAATTCTCGGGTGCCAAGGAACTCCA37840.12388701399556573RNA PCR Primer, Index 1 (100% over 28bp)
CAAACGAGAACTTTGAAGGCCGAAGTGGAATTCTCGGGTGCCAAGGAACT36040.11799386850951873RNA PCR Primer, Index 1 (100% over 25bp)
CGCGACCTCAGATCAGACGTGGCGACCCGTGGAATTCTCGGGTGCCAAGG34500.11295195514923408Illumina Small RNA Adapter 2 (100% over 21bp)
TTCGACCTGAGCTCAGGCAAGGCTACCTGGAATTCTCGGGTGCCAAGGAA33640.11013634119478942RNA PCR Primer, Index 1 (100% over 23bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31720.10385031934300595No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC30680.10044539083995659RNA PCR Primer, Index 1 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTTAG150.002226243369.992195
GCGATTA150.002226243369.992193
TTAGTTC150.002226243369.992193
CGCGTTT150.002226966269.98646519
GTGCATA150.002227469.983029
CATTAAG150.002227469.9830214
AATACGC150.002227469.9830210
GGCTGGT498300.069.766431
GCTGGTC498700.069.757022
TGGTCCG502950.069.296374
CTGGTCC501950.069.2671053
ATATACG3600.069.060781
GGCCATA12750.068.8852547
TGCCCCG64600.068.737214
GTCTACG12350.068.615781
TGTACTG65800.068.556184
TACGGCC12450.068.305634
ACTGAGC66350.068.189937
GCTGCCC65450.068.1652812
GCCCCGA63800.068.1182815
Produced by FastQC (version 0.11.5)