FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005284655

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284655
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3425129
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA1930145.635233008742153No Hit
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA700882.046287891638534RNA PCR Primer, Index 1 (100% over 28bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA505371.4754772739946436RNA PCR Primer, Index 1 (100% over 34bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG284670.8311219810991061RNA PCR Primer, Index 1 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA239380.6988933847455089No Hit
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC207190.6049115230404462RNA PCR Primer, Index 1 (100% over 27bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG105180.3070833244528892Illumina Small RNA Adapter 2 (100% over 21bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA90950.26553744399116064RNA PCR Primer, Index 1 (100% over 28bp)
AGGTTCCGGATAAGTAAGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCA87100.2542969914417822RNA PCR Primer, Index 1 (100% over 28bp)
GTCCCACTCCGTAGATCTGTTCTGGAATTCTCGGGTGCCAAGGAACTCCA86470.2524576446609748RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG83640.24419518213766545No Hit
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC82620.24121719211159637RNA PCR Primer, Index 1 (100% over 27bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC79230.23131975467201382RNA PCR Primer, Index 5 (100% over 35bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC77500.22626885001995548No Hit
TCTTAACCCGGACCAGAAACTATGGAATTCTCGGGTGCCAAGGAACTCCA75630.22080920163882878RNA PCR Primer, Index 1 (100% over 28bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACT74170.2165465884642593RNA PCR Primer, Index 8 (100% over 36bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG69010.2014814624500274No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGTATGC63170.1844310097517495RNA PCR Primer, Index 8 (100% over 50bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC61020.17815387391248622No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG55070.16078226542708318No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGAATTCTCGGGTGCC46910.13695834521853045No Hit
CGCGACCTCAGATCAGACGTGGCGACCTGGAATTCTCGGGTGCCAAGGAA46840.1367539733539963RNA PCR Primer, Index 1 (100% over 23bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC45640.13325045567626795RNA PCR Primer, Index 1 (100% over 31bp)
GGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCAAGGA44390.12960095809530092RNA PCR Primer, Index 1 (100% over 22bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA41690.12171804332041217RNA PCR Primer, Index 1 (100% over 22bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA38140.1113534701904658No Hit
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA37440.10930975154512428RNA PCR Primer, Index 1 (100% over 32bp)
GTGTTCGGAAGATTTTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC37420.1092513595838288RNA PCR Primer, Index 1 (100% over 33bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT36650.10700326907395312No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA35000.10218593226707666RNA PCR Primer, Index 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTGGT255350.069.4936141
GGCCATA33150.069.371837
GCTGGTC256650.069.346212
ACGGCCA32750.069.266335
TACGGCC33100.068.956954
TGCCCCG159700.068.9472814
TGGTCCG262450.068.880784
GTCTACG32950.068.8458941
ACTGAGC160850.068.4385767
GCCCCGA158900.068.4133515
CCCCGAG120300.068.36999516
GCTGCCC160900.068.3688112
CTGGTCC260400.068.2400053
TGTACTG161400.068.1492544
TCTACGG33400.068.127952
CCATACC32750.068.07669
CTGCCCC162050.068.0564113
GAGCTGC162650.068.0420710
TATTGCA29800.068.017511
TACTGAG162300.067.944896
Produced by FastQC (version 0.11.5)