FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005284656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29683994
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTC4200211.4149746829890884No Hit
GTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGG3625071.2212204328029443No Hit
CTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACT3262251.0989929454910954No Hit
CGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATT2701520.9100931633391383No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGT2364870.7966818750872946No Hit
GTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGT2224400.7493600760059445No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGC1912610.6443236715382707No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTC1833490.6176695764053853No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG1749340.5893209653660487No Hit
GTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGT1533630.5166521728848213No Hit
GGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCT1300670.4381721678019474No Hit
GGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCG1150970.3877409488763541No Hit
GGGACTCTTTTGCCAACTTGGGAATGTTCGCGAATATATGCTGAAGCCGT1144950.38571291989885187No Hit
CCTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGG1038980.35001354602079493No Hit
CCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA1027920.34628763231794213No Hit
GGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTG963710.324656446164219No Hit
GTCCTACATATAGTTATAGAATAACGAAGATTCGGCAGGGTAGAGAGTAT948220.3194381456888854No Hit
GGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTT934500.3148161261587642No Hit
GCGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGAT906770.3054743913504362No Hit
GGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGAC805800.2714594269221318No Hit
GTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATT711010.2395263925737217No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA642460.21643313901761335No Hit
GCCCTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGGGAATGTTCGCGAATATATGCTGA632880.21320581051188733No Hit
GAACCGTCAAAGCCAGGCTATCTAAATGGTTTTAAGCGTGGCCCGTATCT622780.20980330342338702No Hit
CACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACA595960.20076813113491399No Hit
GTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGTTTCGTTTCCCCGGGACCTCTTGCCCC592110.19947113585860446No Hit
GTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTT587150.1978002016844499No Hit
GGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATT576950.19436400640695453No Hit
GCCTTCTCTCGTTCTCGCCCGCTTTGCAAAAATATCTAATATCAATTGCG573740.19328261554021336No Hit
GGTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCT561640.18920634467181202No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTT543180.18298750498332536No Hit
GGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTC528320.17798144009866057No Hit
CTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTA508070.17115958182716248No Hit
GTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATA507950.17111915600036842No Hit
GTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCC499580.16829945458148254No Hit
GCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG499510.168275872849186No Hit
GCAGGTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATT495850.16704288513196708No Hit
GTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTC493560.16627142560398037No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTCTAATACTGGTG492760.16600192009201994No Hit
CTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGG484680.16327991442121975No Hit
GTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTG481540.16222210528677508No Hit
CTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGG471970.1589981455999486No Hit
GCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAG457550.15414030874686202No Hit
AGAGAGTATGTTAATTGGGAAATGAAGCAATAATGATGTGGCTTATGCTT451990.15226724543873713No Hit
GTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGGGAATGTTC447970.150912980241136No Hit
TGTAGGGCTGGCCTAAGTCAGGCAGTTCAAGATAACCTGAAGGAGTCGAATAACATCTATCCAGTGAGTCCTGCA440670.1484537424444972No Hit
GTCACTGGGCAGGCGGTGCCTCTAATACTGGTGATGCTAGAGGTGATGTT429680.14475141047394094No Hit
GTTGGTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGG425580.1433701947251438No Hit
CTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATG416780.14040563409357917No Hit
GTGATGATTTGGTAAGAGATAATTTAATAGTACGAGGGCGTTCATGATTC414790.13973523913257763No Hit
TTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGA406680.1370031270050789No Hit
GGCGAATCATAGGGAGGTGAAGGGAGGTGGGGTATAAGGGGAGTGGTGTC396850.13369157802686527No Hit
GGGGTATAAGGGGAGTGGTGTCTAAATTGACCCTGACAACTTACAGTGCGTCTAGGTCGGGGCAGGAAGGAAGTT395520.13324352511323106No Hit
CTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA389220.13112116920654276No Hit
AGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGAC386900.13033960322185756No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA380140.128062281645792No Hit
CCTAAATCAAGACACAGCACCCTTTAATTATACAGACGATGGACAACAATAAAGTGTACAGACCACAGCTCAAAA374610.1261993247943656No Hit
GGGCCCTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAAC371170.12504045109293582No Hit
AGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTT367860.12392537203719958No Hit
GTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCTGCGTTTGTATAGCCGACAAGCGCAATTTGAAGCAC363740.12253741865060341No Hit
GGCTAATTTATATACGTCACGGCTTCTATTCGATGATCCGATATGGTAAA359080.12096754904343397No Hit
GTCCAGCGTGGCAAACAGGAGGTCTTCTTCATAGCTGTCAGCACTCGTCA355830.11987268290109478No Hit
GGCCTTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGGGAATGTTCGCGAATATATGCTG352050.1185992693570818No Hit
GGAGAGGCTATGTGAACAGGAGGAGAGGCTACGTGAACAGGAGGAGAGGC346300.1166621984898663No Hit
CGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGG338770.11412547785853884No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTC337610.11373469486619624No Hit
GCCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATA335030.1128655395901239No Hit
GACCATATAGCATATAAAAATACTTAGTAGTGTGATGATTTGGTAAGAGA334360.11263982872385704No Hit
GGCAGGGTAGAGAGTATGTTAATTGGGAAATGAAGCAATAATGATGTGGC330690.11140347218773863No Hit
CCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTTT330330.11128219470735642No Hit
GAGCGGTGAACCGTCAAAGCCAGGCTATCTAAATGGTTTTAAGCGTGGCCCGTATCTGCAGGGGCGACACGCAG326520.10999867470664493No Hit
GGGCGACACGCCTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTT320830.10808181675282647No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT319600.10766745202818731TruSeq Adapter, Index 13 (97% over 38bp)
AGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATT316370.10657932352364712No Hit
GTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG315400.10625254809039511No Hit
GTAGAGAGTATGTTAATTGGGAAATGAAGCAATAATGATGTGGCTTATGCTTACTGGTAAAAAGACTTTGTAATG315290.10621549108250056No Hit
CGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGC311670.10499597864087966No Hit
GTCAAAGCCAGGCTATCTAAATGGTTTTAAGCGTGGCCCGTATCTGCAGGGGCGACACGCCTGGATCGTGCGAG307810.10369561454567065No Hit
GCCAGAAATGTTGATGCCTTCACAGCGTACGACCACCACCTTCCGGCCCA307030.10343284667150923No Hit
GTCGGAAGATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTA304540.10259401076553243No Hit
GAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGGGAATGTTCGCGAATATATGCTGAAGCCGTGTCTCTGTGG304500.10258053548993441No Hit
GAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGGGAATGTTCGCGA302310.10184276415094275No Hit
CCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATA302190.1018023383241487No Hit
GGGTGGGCAAGTTTGTACCATTTGATCTTAAGATGCTATGCCTCTTTGCA301510.10157325863898235No Hit
ATTATCATCATAAGTAAACAAAAGGCGTCAGACAAAGGATAAACGGTGAA301150.10145198115860014No Hit
CTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGG300700.10130038430812241No Hit
TGTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGT299210.10079843029209613No Hit
GGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATT297100.10008760950430053No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)