FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005284663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2583864
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA29370411.366852125344058No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC745322.8845171417690714No Hit
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA493961.9117105234640834RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG399721.5469854450543836RNA PCR Primer, Index 1 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA373661.4461287436180852No Hit
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA296801.148667267317475RNA PCR Primer, Index 1 (100% over 34bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG224050.8671122009517529Illumina Small RNA Adapter 2 (100% over 21bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG128330.4966592668964001No Hit
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC114440.4429025676273983RNA PCR Primer, Index 1 (100% over 27bp)
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA102630.39719582764417943RNA PCR Primer, Index 1 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCTGGAATTCTCGGGTGCCAAGGAA91600.35450782239312906RNA PCR Primer, Index 1 (100% over 23bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGAATTCTCGGGTGCC86800.3359309932720917No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATGC84650.3276101218949604RNA PCR Primer, Index 5 (100% over 50bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC76800.29722926593659726No Hit
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC75760.2932042862937059RNA PCR Primer, Index 1 (100% over 31bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA75460.292043234473641No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA70940.27455005371799757RNA PCR Primer, Index 1 (100% over 28bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA70830.2741243347173071RNA PCR Primer, Index 1 (100% over 22bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC59990.23217166228563113RNA PCR Primer, Index 5 (100% over 35bp)
CGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACA59570.23054618973754035RNA PCR Primer, Index 5 (100% over 36bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACATGGAATTCTCGGGTGCCA57940.22423780818185476No Hit
AGGTTCCGGATAAGTAAGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCA57940.22423780818185476RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC56680.21936139053758247No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACA54100.20937634488502493RNA PCR Primer, Index 5 (100% over 36bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA53100.20550617215147546No Hit
GTCCCACTCCGTAGATCTGTTCTGGAATTCTCGGGTGCCAAGGAACTCCA51580.19962350959648031RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT51270.19842375604908RNA PCR Primer, Index 1 (100% over 30bp)
TCTTAACCCGGACCAGAAACTATGGAATTCTCGGGTGCCAAGGAACTCCA44920.17384815919104102RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA42820.16572079645058718No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGTGGAATTCTCGGGTGCCAAGG42070.16281816690042508Illumina Small RNA Adapter 2 (100% over 21bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA41870.16204413235371523RNA PCR Primer, Index 1 (100% over 23bp)
GGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCAAGGA39860.15426508515928083RNA PCR Primer, Index 1 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC37890.14664084487418844No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA34970.13533994049222403RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA34390.13309524030676537RNA PCR Primer, Index 1 (100% over 28bp)
CGACTCTTAGCGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCAAGGAA33590.12999910211992582RNA PCR Primer, Index 1 (100% over 23bp)
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG33550.12984429521058383RNA PCR Primer, Index 1 (100% over 29bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATGTCGTATGC32850.12713517429709922RNA PCR Primer, Index 5 (98% over 50bp)
CGCGACCTCAGATCAGACGTGGCGACCCTGGAATTCTCGGGTGCCAAGGA32700.12655464838706681RNA PCR Primer, Index 1 (100% over 22bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTTGGAATTCTCGGGTGC30010.1161438837338188No Hit
GCTCTACACTCTACTCGTCAGCTGTTGGAATTCTCGGGTGCCAAGGAACT28200.10913887108609432RNA PCR Primer, Index 1 (100% over 25bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGC26440.10232736707504729No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTTGGAATTCTCGGGTGCCAAGGA26030.10074059625429202RNA PCR Primer, Index 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCTA207.932215E-569.9969215
GGCTGGT366000.069.735691
GCTGGTC366550.069.7185752
CTACGGC65550.069.683313
TCTACGG65500.069.6309742
TACGGCC65700.069.630774
GTCTACG65650.069.54271
TTTCCGT99650.069.443042
TGGTCCG370650.069.427654
TTCCGTA100200.069.235193
GTTTCCG101250.069.227351
CTGGTCC369200.069.2262953
ACGGCCA66750.069.16475
TCACGTT98550.068.9670422
TTCGCCT101400.068.9296227
TTATCAC101100.068.8864919
CGTTCGC98800.068.83060525
ACGTTCG98850.068.82854524
CATACCA65900.068.82587410
TGCCCCG127750.068.8188914
Produced by FastQC (version 0.11.5)