FastQCFastQC Report
Wed 8 May 2024
EGAF00005284668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5007784
Total Bases380.5 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA148133029.580549001314754No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC2208594.410314023128794No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA1939683.873329999856224No Hit
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA1562213.1195634635998677RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG1304992.605923098919602No Hit
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC688081.3740209242251662RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA544261.0868280261289225No Hit
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG530671.059690274181155RNA PCR Primer, Index 1 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA514451.0273006982729287No Hit
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC417740.8341813464798002RNA PCR Primer, Index 2 (100% over 34bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG380440.7596973032383185Illumina Small RNA Adapter 2 (100% over 21bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG256430.5120628206008886Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCTGGAATTCTCGGGTGCCAAGGAA222790.4448873992967748RNA PCR Primer, Index 1 (100% over 23bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA222520.4443482386620509No Hit
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC218830.43697970998749147RNA PCR Primer, Index 1 (100% over 27bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC206950.4132566420596415RNA PCR Primer, Index 1 (100% over 31bp)
GGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCAAGGA202950.40526907710076954RNA PCR Primer, Index 1 (100% over 22bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC195110.38961344978138035No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG165120.32972668150223733No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA155000.3095181421562911RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA134620.2688214986908381RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGAATTCTCGGGTGCC134350.26828233805611423No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA120310.24024598505047343No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTTGGAATTCTCGGGTGCCAAGG115350.2303414045014721Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG111970.22359191211122525No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA111290.22223402606821702RNA PCR Primer, Index 1 (100% over 23bp)
CGACTCTTAGCGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCAAGGAA110670.22099595349959184RNA PCR Primer, Index 1 (100% over 23bp)
GGCTCCGTGGCGCAATGGATAGCGCATTGGACTGGAATTCTCGGGTGCCA103880.20743706198190656No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA94990.1896846988608135No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA93400.18650964178966184RNA PCR Primer, Index 1 (100% over 22bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC87950.17562658453319874No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA86900.1735298487314948No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA86400.1725314031116358RNA PCR Primer, Index 1 (100% over 23bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC86090.17191236682732322RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC84240.1682181180338449RNA PCR Primer, Index 1 (100% over 26bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA80440.16062993132291647No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAC74750.14926762016892103RNA PCR Primer, Index 30 (100% over 36bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA70350.1404812987141618RNA PCR Primer, Index 7 (100% over 35bp)
GTTTCCGTAGTGTAGTGGTTATCACTTTCGCCTTGGAATTCTCGGGTGCC69710.13920328832074227No Hit
TGAGGGGCAGAGAGCGAGACTTTTGGAATTCTCGGGTGCCAAGGAACTCC68670.13712652143143553RNA PCR Primer, Index 1 (100% over 27bp)
AGGTTCCGGATAAGTAAGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCA64160.12812054194030734RNA PCR Primer, Index 1 (100% over 28bp)
GGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAAGGAA63750.12730181653202294RNA PCR Primer, Index 1 (100% over 23bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG61240.12228961952033075No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACATGGAATTCTCGGGTGCCA61110.12203002365916742No Hit
TCTTAACCCGGACCAGAAACTATGGAATTCTCGGGTGCCAAGGAACTCCA58930.11767680075658216RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA55210.11024836534483118No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGTGGAATTCTCGGGTGCCAAGG54570.10897035495141165Illumina Small RNA Adapter 2 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTATCGGGTGCCA52670.10517626159594744No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC50930.10170167083883809RNA PCR Primer, Index 1 (100% over 26bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA50110.10006422002226933RNA PCR Primer, Index 1 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)