FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005284669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76331165
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGC4392053157.539448009210915RNA PCR Primer, Index 12 (100% over 50bp)
GTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTA15934932.087604715583733RNA PCR Primer, Index 12 (100% over 39bp)
AGAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCA12682061.6614524355811942RNA PCR Primer, Index 1 (100% over 28bp)
AGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGT12247381.6045058397837895RNA PCR Primer, Index 1 (100% over 30bp)
GAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAG6372970.834910616129074RNA PCR Primer, Index 1 (100% over 29bp)
TACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT6341860.8308349545038386RNA PCR Primer, Index 1 (97% over 35bp)
CAGAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCC5798340.7596294383820815RNA PCR Primer, Index 1 (100% over 27bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT5207590.6822364102526144RNA PCR Primer, Index 1 (97% over 35bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4739410.620901043499074No Hit
CTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC3912000.5125036412060526RNA PCR Primer, Index 2 (100% over 34bp)
GTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC3577590.4686932264167591RNA PCR Primer, Index 1 (100% over 31bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA3574830.4683316440932088No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATATCGTATGC3122700.4090989571559664RNA PCR Primer, Index 12 (98% over 50bp)
GGGGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC3117820.4084596376853412RNA PCR Primer, Index 12 (100% over 44bp)
ACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTT2687760.3521182992556186RNA PCR Primer, Index 12 (100% over 36bp)
GGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGC2685100.35176981774089255RNA PCR Primer, Index 12 (98% over 50bp)
TTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA2558210.3351462014237566RNA PCR Primer, Index 1 (100% over 32bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTATCTCGTATGCC2549420.3339946403281019RNA PCR Primer, Index 12 (97% over 39bp)
TCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC2167300.28393382964874175RNA PCR Primer, Index 1 (100% over 33bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC2002810.2623843092136744RNA PCR Primer, Index 2 (100% over 34bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAAGCTCGTATGC1615430.21163439599015685RNA PCR Primer, Index 12 (98% over 50bp)
TCAGAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTC1543060.20215334064402132RNA PCR Primer, Index 1 (100% over 26bp)
AGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGC1462070.19154299557723245RNA PCR Primer, Index 12 (98% over 50bp)
CGGCTCGAAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTG1355630.17759849466466285RNA PCR Primer, Index 12 (100% over 37bp)
CAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTG973860.12758353681618773RNA PCR Primer, Index 12 (100% over 37bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATGTCGTATGC933540.12230129069823578RNA PCR Primer, Index 12 (98% over 50bp)
CGGACGAGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTA908570.11903001873481167RNA PCR Primer, Index 12 (100% over 39bp)
GGCTCGAAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGT849340.1112704096681873RNA PCR Primer, Index 12 (100% over 38bp)
TGGAATTCTCGGGTGCCAGGAACTCCAGTCACCTTGTAATCTCGTATGCC845530.11077126885198202RNA PCR Primer, Index 12 (97% over 35bp)
GACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCT839520.10998391024164246RNA PCR Primer, Index 12 (100% over 43bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG45677100.069.0549570
AAAAAGG46136100.068.7428469
GCTGGTC459300.068.728832
CCTGTAC119250.068.702392
GCTGCCC118450.068.6532412
CTGTACT118950.068.608853
AAAAAAG46773500.068.5643268
TCCTGTA118550.068.551331
CTGCCCC118150.068.4724813
GTCTACG77250.068.441691
TACGGCC77350.068.362984
TGCCCCG118650.068.3019914
ATGTTAC25850.068.2226911
TGTTACA29350.068.0750512
CTGGTCC462400.067.933043
TGGTCCG463500.067.915414
AAAAGGC157600.067.8138470
GCCCCGA117900.067.7866215
CTACGGC78250.067.576573
AAAAGTG292150.067.5205270
Produced by FastQC (version 0.11.5)