FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005284671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1977612
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA909954.601256464867729RNA PCR Primer, Index 1 (100% over 32bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA558122.82219161291497No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATTCTCGGGTGCCAAGG313811.5868127822848972Illumina Small RNA Adapter 2 (100% over 21bp)
AAATGGATTTTTGGAGCAGGGAGATGGAATTCTCGGGTGCCAAGGAACTC269261.3615410909723444RNA PCR Primer, Index 1 (100% over 26bp)
TGGTTAGTACTTGGATGGGAGACCGCCTGGAATTCTCGGGTGCCAAGGAA236341.1950776997712393RNA PCR Primer, Index 1 (100% over 23bp)
CTTTCGAGGCCCTGTAATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA212981.0769554391862508RNA PCR Primer, Index 1 (100% over 32bp)
CTGAGAAGACGGTCGAACTTGACTATCTAGTGGAATTCTCGGGTGCCAAG201691.0198663843059204No Hit
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG195310.9876052532043698RNA PCR Primer, Index 6 (100% over 34bp)
CAAACGAGAACTTTGAAGGCCGAAGTGGAATTCTCGGGTGCCAAGGAACT188190.9516022354233288RNA PCR Primer, Index 1 (100% over 25bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATTCTCGGGTGCCAAGGA171760.8685222379314042RNA PCR Primer, Index 1 (100% over 22bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC170980.8645780871070766RNA PCR Primer, Index 1 (100% over 27bp)
CAACTCTTAACAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTT155280.785189410258433RNA PCR Primer, Index 21 (100% over 37bp)
AAACGAGAACTTTGAAGGCCGAAGTGGAATTCTCGGGTGCCAAGGAACTC126650.6404188485911291RNA PCR Primer, Index 1 (100% over 26bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA115800.585554699304009No Hit
AATGGATTTTTGGAGCAGGGAGATGGAATTCTCGGGTGCCAAGGAACTCC66450.3360113106109793RNA PCR Primer, Index 1 (100% over 27bp)
AAATGGATTTTTGGAAATAGGAGATGGAATTCTCGGGTGCCAAGGAACTC64110.32417885813799674RNA PCR Primer, Index 1 (100% over 26bp)
AAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGAATTCTCGGGTGCCAAGG62290.3149758395478992Illumina Small RNA Adapter 2 (100% over 21bp)
CAGGCCTATGCTCTGAATACATTAGCATGGAATTCTCGGGTGCCAAGGAA58990.2982890475988212RNA PCR Primer, Index 1 (100% over 23bp)
GCAGGCCTATGCTCTGAATACATTAGCATGGAATTCTCGGGTGCCAAGGA56820.2873162177413972RNA PCR Primer, Index 1 (100% over 22bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA53750.27179244462513374RNA PCR Primer, Index 1 (100% over 28bp)
AGCAGGCCTATGCTCTGAATACATTAGCATGGAATTCTCGGGTGCCAAGG52380.2648648976644559Illumina Small RNA Adapter 2 (100% over 21bp)
TGAGGGGCAGAGAGCGAGACTTTTGGAATTCTCGGGTGCCAAGGAACTCC50820.25697659601580086RNA PCR Primer, Index 1 (100% over 27bp)
GCCGAATTGTGGTCTGTAGACTGTGGCATTACTGGAATTCTCGGGTGCCA43530.22011395561920133No Hit
ATGGATTTTTGGAGCAGGGAGATGGAATTCTCGGGTGCCAAGGAACTCCA42140.21308527658610485RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC39410.19928074870095852RNA PCR Primer, Index 1 (100% over 27bp)
AAGCAGGCCTATGCTCTGAATACATTAGCATGGAATTCTCGGGTGCCAAG36430.18421206991057903No Hit
CAATTGAGCGTTGGAAATTGAAGACTGAAAGTGGAATTCTCGGGTGCCAA36370.18390867369332306No Hit
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT33750.17066037220647934RNA PCR Primer, Index 1 (100% over 30bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGGAATTCTCGGGTGCCAAGGA32380.1637328252458015RNA PCR Primer, Index 1 (100% over 22bp)
TGTAAACATCCCCGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG32190.16277207055782428RNA PCR Primer, Index 6 (100% over 34bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC31550.15953584424042735No Hit
TGTGATCAAGCTAGTAAGAGCTTACGGTGGAATTCTCGGGTGCCAAGGAA31390.1587267876610781RNA PCR Primer, Index 1 (100% over 23bp)
ATTCAAACGAGAACTTTGAAGGCCGAAGTGGAATTCTCGGGTGCCAAGGA31250.15801886315414754RNA PCR Primer, Index 1 (100% over 22bp)
GTAACAGATCTTCTGTAGACGACTTAACATGGAATTCTCGGGTGCCAAGG29660.1499788633968645Illumina Small RNA Adapter 2 (100% over 21bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT29500.14916980681751527RNA PCR Primer, Index 1 (100% over 30bp)
AACAGATCTTCTGTAGACGACTTAACATGGAATTCTCGGGTGCCAAGGAA27890.1410286749878136RNA PCR Primer, Index 1 (100% over 23bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA26960.13632603362034615RNA PCR Primer, Index 1 (100% over 28bp)
GAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATTCTCGGGTGCCAAG26090.1319267884701347No Hit
GTTGAATTGTGGTCTGTGGACGTGGAATTCTCGGGTGCCAAGGAACTCCA25840.1306626375649015RNA PCR Primer, Index 1 (100% over 28bp)
ATCTTCTGTAGACGACTTAACATGGAATTCTCGGGTGCCAAGGAACTCCA25020.12651622259573667RNA PCR Primer, Index 1 (100% over 28bp)
GAGAAGACGGTCGAACTTGACTATCTAGTGGAATTCTCGGGTGCCAAGGA24360.12317886420592108RNA PCR Primer, Index 1 (100% over 22bp)
AACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA23870.12070112843166406RNA PCR Primer, Index 1 (100% over 28bp)
AGCAGGACGGTGGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG23480.11872905301950028RNA PCR Primer, Index 6 (100% over 34bp)
ACGAGAACTTTGAAGGCCGAAGTGGAATTCTCGGGTGCCAAGGAACTCCA23390.11827395869361634RNA PCR Primer, Index 1 (100% over 28bp)
TGCTCTGAATACATTAGCATGGAATTCTCGGGTGCCAAGGAACTCCAGTC22450.11352075128993958RNA PCR Primer, Index 1 (100% over 31bp)
GAGGCCCTGTAATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTT22150.11200377020365976RNA PCR Primer, Index 21 (100% over 37bp)
GTAGCAATATCTGTTTAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA21830.1103856570449613RNA PCR Primer, Index 1 (100% over 32bp)
AGGCCTATGCTCTGAATACATTAGCATGGAATTCTCGGGTGCCAAGGAAC21140.10689660054651771RNA PCR Primer, Index 1 (100% over 24bp)
GCTTTCGGGCTGGGTTGGACTTTGTTGAAATTGGAATTCTCGGGTGCCAA20830.10532905342402858No Hit
AAAAAGCGGAATTGTAATCTATGGAATTCTCGGGTGCCAAGGAACTCCAG20800.1051773553154006RNA PCR Primer, Index 1 (100% over 29bp)
TGTAACAGATCTTCTGTAGACGACTTAACATGGAATTCTCGGGTGCCAAG20410.10320527990323683No Hit
ATTATTGTAACAGATCTTCTGTAGACGACTTAACATGGAATTCTCGGGTG20410.10320527990323683No Hit
ACAGATCTTCTGTAGACGACTTAACATGGAATTCTCGGGTGCCAAGGAAC20370.10300301575839953RNA PCR Primer, Index 1 (100% over 24bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA20090.10158716674453837No Hit
ATGCTCTGAATACATTAGCATGGAATTCTCGGGTGCCAAGGAACTCCAGT20080.10153660070832903RNA PCR Primer, Index 1 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)