FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005284677

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284677
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3761006
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA74266419.74641891025965No Hit
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG1333593.545833215900214RNA PCR Primer, Index 6 (100% over 34bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA785562.0886964817391944No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA328070.8722932109121869RNA PCR Primer, Index 1 (100% over 28bp)
GGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCAAGGA311060.8270659499080832RNA PCR Primer, Index 1 (100% over 22bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA294900.7840987225226442RNA PCR Primer, Index 1 (100% over 28bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGG226640.6026047286284574RNA PCR Primer, Index 2 (97% over 35bp)
AAGATTAAGAGAACCAACACCTTGGAATTCTCGGGTGCCAAGGAACTCCA222200.5907993765497849RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG206380.5487361626118119RNA PCR Primer, Index 1 (100% over 29bp)
CTTTGGACACTAGGAAAAAACCTGGAATTCTCGGGTGCCAAGGAACTCCA181530.4826634150543764RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC180680.48040338143571165RNA PCR Primer, Index 1 (100% over 27bp)
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA143790.3823179223856596RNA PCR Primer, Index 1 (100% over 23bp)
ATATACGGAGAGTTTGATTCTGGCTCTGGAATTCTCGGGTGCCAAGGAAC137190.3647694260524976RNA PCR Primer, Index 1 (100% over 24bp)
AAAGATTAAGAGAACCAACACCTTGGAATTCTCGGGTGCCAAGGAACTCC128380.3413448423108073RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC113120.30077059169807224RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC105120.2794996870518154RNA PCR Primer, Index 1 (100% over 27bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA101540.26998095722261545RNA PCR Primer, Index 1 (100% over 22bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGC100340.2667903215256769RNA PCR Primer, Index 6 (97% over 36bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC94080.2501458386399809RNA PCR Primer, Index 1 (100% over 33bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGAATTCTCGGGTGCC92260.24530670783295747No Hit
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG89820.2388190819158491Illumina Small RNA Adapter 2 (100% over 21bp)
GGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAAGGAA85880.2283431613775676RNA PCR Primer, Index 1 (100% over 23bp)
AGGTTCCGGATAAGTAAGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCA84280.2240889804483162RNA PCR Primer, Index 1 (100% over 28bp)
TCTTAACCCGGACCAGAAACTATGGAATTCTCGGGTGCCAAGGAACTCCA82010.21805336125494085RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA82000.218026772624133RNA PCR Primer, Index 1 (100% over 28bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG81590.21693663876101235No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA81260.21605921394435423RNA PCR Primer, Index 1 (100% over 23bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA79430.21119349450652297RNA PCR Primer, Index 1 (100% over 32bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC78640.2090929926727051RNA PCR Primer, Index 1 (100% over 27bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC72700.1932993459728594RNA PCR Primer, Index 1 (100% over 24bp)
GTCCCACTCCGTAGATCTGTTCTGGAATTCTCGGGTGCCAAGGAACTCCA64700.1720284413266025RNA PCR Primer, Index 1 (100% over 28bp)
TTCGACCTGAGCTCAGGCAAGGCTACCCGTGGAATTCTCGGGTGCCAAGG64580.17170937775690867Illumina Small RNA Adapter 2 (100% over 21bp)
CAAACGAGAACTTTGAAGGCCGAAGTGGAATTCTCGGGTGCCAAGGAACT63440.16867827384481707RNA PCR Primer, Index 1 (100% over 25bp)
ATACGATGAAGAGTTTGATCCTGGCTCTGGAATTCTCGGGTGCCAAGGAA61980.16479633374687516RNA PCR Primer, Index 1 (100% over 23bp)
ATATACGGAGAGTTTGATTCTGGCTCATGGAATTCTCGGGTGCCAAGGAA60960.16208429340447741RNA PCR Primer, Index 1 (100% over 23bp)
TTCGACCTGAGCTCAGGCAAGGCTACCCTGGAATTCTCGGGTGCCAAGGA48580.1291675684643949RNA PCR Primer, Index 1 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCTGGAATTCTCGGGTGCCAAGGAA48370.12860920721743066RNA PCR Primer, Index 1 (100% over 23bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC47670.12674800306088319RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA47070.1251526852124139RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGC46400.12337124694828991RNA PCR Primer, Index 11 (100% over 50bp)
CGCGACCTCAGATCAGACGTGGCGACCCGTGGAATTCTCGGGTGCCAAGG43340.11523512592109665Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCTGGAATTCTCGGGTGCCAAGGA42910.11409181479636032RNA PCR Primer, Index 1 (100% over 22bp)
GTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCAAGGAA39050.10382860330454138RNA PCR Primer, Index 1 (100% over 23bp)
CGGACAGGATTGACAGATTGATAGCTTGGAATTCTCGGGTGCCAAGGAAC38620.10268529217980508RNA PCR Primer, Index 1 (100% over 24bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG38580.1025789376565738No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTATCGGGTGCCA38260.10172810147072353No Hit
CGAAGAAGCAGCTTCAAACCTGCCGGGGCTTTGGAATTCTCGGGTGCCAA38040.10114315159295145No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTGGT931300.069.906441
TGGTCCG941500.069.781164
CTGGTCC931950.069.77883
GCTGGTC934500.069.764462
ATATACG27400.068.989621
TGCCCCG86850.068.9124314
TATTGCA17850.068.835021
GCCCCGA85800.068.8156915
GCTGCCC87400.068.7190412
CTGCCCC87300.068.5572113
TACGGCC23150.068.499564
CTGTACT87800.068.297283
ACTGAGC87950.068.250427
TGTACTG88250.068.187024
TCCTGTA88150.068.184951
CCTGTAC89850.068.102632
GTCTACG23200.068.0501561
CCCCGAG60300.068.0252216
GAGCTGC88400.068.0208710
CAGTTGA43150.067.972698
Produced by FastQC (version 0.11.5)