FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005284690

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284690
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33588618
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5424591.6150083936171473No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGT2044550.6087032220259851No Hit
GCCTACAGCACCCGGTATTCCCAGGCGGTCTCCCATCCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCG1811350.5392749412911243No Hit
GGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTC1458350.43417981650808024No Hit
GTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGT1394950.41530437483316524No Hit
GTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGTTTCGTTTCCCCGGGACCTCTTGCCCC1388760.413461488650709No Hit
GTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGG1249480.37199506094594303No Hit
GCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCG1137200.33856707054752894No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGC1119230.33321704394030144No Hit
CTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACT1108360.32998082862474426No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGC1044410.3109416410046999No Hit
CGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATT1008870.30036067575033903No Hit
CCTAAATCAAGACACAGCACCCTTTAATTATACAGACGATGGACAACAATAAAGTGTACAGACCACAGCTCAAAA947250.2820151754978427No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTC889070.26469383170215577No Hit
GTGATGATTTGGTAAGAGATAATTTAATAGTACGAGGGCGTTCATGATTC871870.25957304941810944No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA856820.2550923649195689No Hit
GGCTAATTTATATACGTCACGGCTTCTATTCGATGATCCGATATGGTAAA828280.24659543896685476No Hit
ATCGGGTCCAGCGTGGCAAACAGGAGGTCTTCTTCATAGCTGTCAGCACT770950.22952715708636778No Hit
GCCTTCTCTCGTTCTCGCCCGCTTTGCAAAAATATCTAATATCAATTGCG760180.22632071376083412No Hit
GGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGAC755250.2248529546526743No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC752800.22412354089709793No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT727980.216734132973259TruSeq Adapter, Index 7 (97% over 36bp)
GGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCT697360.20761794962805552No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG693190.20637645764407456No Hit
GTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGT650900.19378588306312575No Hit
ATGAGTATGGTTTAGGGCCCTGGATCGTGCGAGAGAAAGAAAACCATGGG623620.18566408418470803No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTCTAATACTGGTG590340.17575596590487885No Hit
GCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGGGAATGTTCGC582250.17334741191197567No Hit
GAACCGTCAAAGCCAGGCTATCTAAATGGTTTTAAGCGTGGCCCGTATCT581410.17309732719577806No Hit
GGGACTCTTTTGCCAACTTGGGAATGTTCGCGAATATATGCTGAAGCCGT581070.17299610242969807No Hit
CCTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGG570610.16988195227323732No Hit
CGACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCG565340.16831296839899754No Hit
GTAAAACGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGT562450.16745255788731767No Hit
GCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGT558140.16616938511730373No Hit
GTCTGCGATAGGCTAGTTCATAAACGAGGGGCGATGCCCGGGCTCAGAATCCAAGGAGCAAAACTGCGTCGGTTT551100.16407343701964755No Hit
CTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTGTCGATGATCAATGTGTCCTGC542510.1615160230766267No Hit
CACATAACATAACAGATCCTAAATCAAGACACAGCACCCTTTAATTATAC540970.16105753443026444No Hit
CCAGAATTCAAGACGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCT536620.1597624528642411No Hit
CTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA532310.15847928009422715No Hit
CTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATAT532180.15844057650719656No Hit
GACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGT528830.15744321484140847No Hit
GTCTCTGTGGACGGGAAAGGGCGAATCATAGGGAGGTGAAGGGAGGTGGG528440.15732710408031672No Hit
GGGGATTCGGCGCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAA525320.15639821799158274No Hit
GTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATT521690.15531749475372877No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGC520590.15499000286347No Hit
GGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTG509710.15175081034891044No Hit
GGAAGGAATAAAACACTGTAGAGAAGCTATTCGATGTCCTACATATAGTT508900.1515096572297199No Hit
AGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGGGAATGTTCGCGAA508850.15149477123470814No Hit
GTGAAAATTAACAAGAAGCAATACAGAGTATGGTTTAGGGCCCTGGATCG506010.15064924671804003No Hit
GCTCAGACAGGCGTAGCCCCGGGAGGACCCCGGGGCCGCAAGTGCGTTCG505270.15042893399186594No Hit
GGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCCAG497200.14802633439696744No Hit
GGAAGATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATTTTTCTGGTC494440.14720462747231816No Hit
GTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCTGCGTTTGTATAGCCGACAAGCGCAATTTGAAGCAC488830.14553441883199839No Hit
GCCCTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGGGAATGTTCGCGAATATATGCTGA481560.1433699951572881No Hit
GCGACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGC481050.14321815800816812No Hit
CCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAAT473940.14110136951749547No Hit
GCTAATTTATATACGTCACGGCTTCTATTCGATGATCCGATATGGTAAAATTTATTCAGACTGTAAAGTAGGACC468820.139577043628291No Hit
CGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTC463100.13787408579894533No Hit
GGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTT463090.137871108599943No Hit
GGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTC462090.13757338869970775No Hit
CGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGC460870.13721017042142072No Hit
GGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCG456230.13582875008432915No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTT454860.13542087382100687No Hit
GTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCG451610.1344532841452423No Hit
CCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA447540.13324156415128482No Hit
CGTGTCTCTGTGGACGGGAAAGGGCGAATCATAGGGAGGTGAAGGGAGGTGGGGTATAAGGGGAGTGGTGTCTAA445990.1327800983059202No Hit
GCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTT434390.12932654746319125No Hit
GTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCC429700.12793024113108792No Hit
CTGAAGCCGTGTCTCTGTGGACGGGAAAGGGCGAATCATAGGGAGGTGAA429280.1278051987729891No Hit
GTCGGAAGATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTA418050.12446180429334724No Hit
GTCCTACATATAGTTATAGAATAACGAAGATTCGGCAGGGTAGAGAGTAT416450.12398545245297082No Hit
GTGGCAAACAGGAGGTCTTCTTCATAGCTGTCAGCACTCGTCAGGCGGTT415700.1237621625277944No Hit
TGTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGT411360.1224700581607734No Hit
CTCAGACGCTGCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA405560.12074328273940893No Hit
CTCAAGCGTTGAGCGGAATGCAGCAATCAATGTCGTCGGAAGATCCTGAATAAATCCTACTGTATCTGAAAGA400020.11909391449210563No Hit
GTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAG397180.11824838997543752No Hit
GTATGGTTTAGGGCCCTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGGGAATGTTCGCG394840.11755172540888702No Hit
GCAGGAAGGAAGTTACTAGGCTAATTTATATACGTCACGGCTTCTATTCG391280.11649184256404953No Hit
CTAACATTATTGCTCAGCGTTCTTGCAGGGGCTAATCTTTGAGGCAGTCATGTCCACACAGATCGCGAAAGATTA390240.11618221386780488No Hit
GTTCCTGTTGATACCGTCTGCGATAGGCTAGTTCATAAACGAGGGGCGATGCCCGGGCTCAGAATCCAAGGAGCA387460.11535455254515087No Hit
TGTAAATAAATAGGAATGAGAATTAAACAGTGGAAGGAATAAAACACTGTAGAGAAGCTATTCGATGTCCTACAT383310.11411901495917458No Hit
CGTTTGTATAGCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTTTCTT367900.10953115129654933No Hit
AAATCAAGACACAGCACCCTTTAATTATACAGACGATGGACAACAATAAA366200.1090250274661494No Hit
GTAAGAGATAATTTAATAGTACGAGGGCGTTCATGATTCAAGCGTCTAAGAGAGTAATGAGGATGCAAGGGTG364840.10862012840182945No Hit
GTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTC363390.10818843454648834No Hit
AGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGAC362470.10791453223827191No Hit
GGGCCCTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAAC362390.10789071464625309No Hit
GTTGATTTCCCTGCGTTTGTATAGCCGACAAGCGCAATTTGAAGCACACC361210.10753940516397549No Hit
CTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGG360560.10734588722882259No Hit
GCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGG354730.10561018021045104No Hit
GTCACTGGGCAGGCGGTGCCTCTAATACTGGTGATGCTAGAGGTGATGTT353790.10533032350422993No Hit
GTGGTGATGATTTTCAAATTTAATACACTTAACTAAAAAATAAGAAATTC349270.10398462955516657No Hit
GCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTTTCTTCTTTCACGGT346670.10321055781455492No Hit
GTGCCATTCGGACCTACCGTAAGCCTATATTTCGTTTTTCTGAGACCTAT344860.10267168479512911No Hit
CAGCAATCAATGTCGTCGGAAGATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGC344680.10261809521308676No Hit
CTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAGTA343300.10220724175076211No Hit
GTCATTTAGAATTAATCGAGTCAAGAAATAGCGTAGCACCATTATCATCATAAGTAAACAAAAGGCGTCAGACAA341390.10163859674131277No Hit
GTCAAGAAATAGCGTAGCACCATTATCATCATAAGTAAACAAAAGGCGTCAGACAAAGGATAAACGGTGAAAATT341320.10161775634829633No Hit
GGGCGAATCATAGGGAGGTGAAGGGAGGTGGGGTATAAGGGGAGTGGTGT341100.10155225797024456No Hit
GTCATGACTGGACACTGCATCGGAAGACACTTCGTGCCGACACGAAGCAG340860.1014808051941881No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)