FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005284691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33588618
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTT4071941.2122975705639334No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA3595231.070371516922786No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCA2651010.7892584327226563No Hit
GCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGG1616350.4812195607452501No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1409840.41973742414766807No Hit
TGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTG1370380.40798939688438507No Hit
GATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCC1098730.32711378598547874No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT919690.27381001504735925No Hit
AGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCT890480.2651136167614875No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA846450.2520050095541293No Hit
ATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGT811400.24156992705088373No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT809600.24103403123046027No Hit
CGATGAAGAACGCAGCTAGCTGCGAGAATTAATGTGAATTGCAGGACACA702830.20924647748234237No Hit
GAACGAGAGAAGGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCA699950.20838904416966486No Hit
GTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGT680250.2025239621350304No Hit
CAATAACGTCTCTTTCAGAAGGCATTGGTATCTTTTCCCCACTTCCAAGC675700.20116933658896No Hit
CTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTT652700.19432177888354918No Hit
AGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTC638130.18998399993712156No Hit
AAAACGAATAGCTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCC636730.1895671920767922No Hit
AGAGAAGGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCACTGCC636250.18942428652467927No Hit
CATGTGCTAATGTTTCTCTCACCCTTGCATCCTCATTACTCTCTTAGACGCTTGAATCATGAACGCCCTCGTACT627370.18678053381059023No Hit
AAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCC612610.18238618808311793No Hit
CGTATATAAATTAGCCTAGTAACTTCCTTCCTGCCCCGACCTAGACGCAC580900.17294549004665807No Hit
CTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACT579680.17258227176837104No Hit
ACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTAGTACTTGGATGGG574920.17116512504325126No Hit
AAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTTAATTCCCCT566110.1685422127221787No Hit
CGAGAGAAGGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCA560630.1669107076688895No Hit
AGTAACTTCCTTCCTGCCCCGACCTAGACGCACTGTAAGTTGTCAGGGTC553450.1647730787852004No Hit
CTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAATGTG551400.1641627529897181No Hit
AAAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTTA543250.1617363358028008No Hit
GGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACC541300.16115578199734207No Hit
GCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTAGTACTTGG516070.15364430891440667No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT515960.1536115597253808No Hit
CTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCAC513090.1527571036117056No Hit
CCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCA511390.15225097978130567No Hit
CTTCCTTCCTGCCCCGACCTAGACGCACTGTAAGTTGTCAGGGTCAATTT503250.14982753979339072No Hit
GGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAA495050.14738623661146166No Hit
GGAAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCA494040.14708553951222406No Hit
ATTTAGACACCACTCCCCTTATACCCCACCTCCCTTCACCTCCCTATGAT493030.14678484241298645No Hit
GCTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCA489520.1457398455631607No Hit
CCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTTAATTCCCCTATGGTCCTACTTTACAGTCTGAAT484080.14412024930588094No Hit
TATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAA479740.14282814493885995No Hit
CACTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAATG479660.14280432734684112No Hit
AACGAGAGAAGGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCAC472400.1406428808711332No Hit
GATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGT460680.13715360364037602No Hit
AGCTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTC457870.13631701072071498No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACC447590.13325645014629658No Hit
GAACGCAGCTAGCTGCGAGAATTAATGTGAATTGCAGGACACATTGATCA446490.1329289582560378No Hit
GGGAAACGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGCATTCAT442530.1317499874511062No Hit
AGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAG440710.13120813723267805No Hit
CACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTAGTACTTGGATGGG439290.130785374974344No Hit
ATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGC436430.12993389605967118No Hit
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG429770.1279510815241044No Hit
GGCCGATTGTCGCAGCCCGGGTCGATTATAACAACGGTGCAATCTCAGCTAAACCGACGCAGTTTTGCTCCTTGG427630.12731396093760095No Hit
CAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAG417940.12442905510432135No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT415700.1237621625277944No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT409990.12206218189745109No Hit
AGAACGAGAGAAGGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCACTGCCGCGTCTGACGGGACAAGGG409770.12199668351939935No Hit
GTCCCGGGGAAACGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGC399540.1189510089399927No Hit
AATTTATTCAAGAGAAGGTAAAGAACACTTGCACACATGTGCTAATGTTT397910.11846572550260924No Hit
GAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTT387660.11541409652519792No Hit
TGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAAT387020.11522355578904736No Hit
CAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTC384750.11454773161551333No Hit
CGAATAGCTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAG384470.11446437004344746No Hit
AGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCACAC384380.11443757525242627No Hit
AATTGGGGCAAGAGGTCCCGGGGAAACGAAACTGGAAACCGACCGCCGCC382190.11378556867091107No Hit
GTCAGGGTCAATTTAGACACCACTCCCCTTATACCCCACCTCCCTTCACC381550.1135950279347605No Hit
TCAGGGTCAATTTAGACACCACTCCCCTTATACCCCACCTCCCTTCACCTCCCTATGATTCGCCCTTTCCCGTCC379860.11309188130336294No Hit
CCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACT375620.11182954892636549No Hit
AGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAG375600.11182359452836077No Hit
ACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCA375380.11175809615030902No Hit
CAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTGAAAGA364010.10837302088463419No Hit
GAAGAACGCAGCTAGCTGCGAGAATTAATGTGAATTGCAGGACACATTGA363810.10831347690458713No Hit
GGACGGGCACGCTCATATCAGGCTATATTTGGTCCGGGTTATTATCGTCG361130.10751558757195667No Hit
ATTCTATGGAAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCACACAAAAACGGA355030.10569949618052163No Hit
AGATGATTGACCGCACGCAATTGATATTAGATATTTTTGCAAAGCGGGCG354610.10557445382242281No Hit
GCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACG353950.10537795868826756No Hit
CATCACACTACTAAGTATTTTTATATGCTATATGGTCACTCTCTGTTATG351260.10457709215663472No Hit
GTCTGAATAAATTTTACCATATCGGATCATCGAATAGAAGCCGTGACGTA350230.10427044065939241No Hit
GTCATTTAGACACCACTCCCCTTATACCCCACCTCCCTTCACCTCCCTATGATTCGCCCTTTCCCGTCCACAG339980.10121881168198107No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)