FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005284693

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284693
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2635169
Sequences flagged as poor quality0
Sequence length76
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTAACCCGGACCAGAAACTATGGAATTCTCGGGTGCCAAGGAACTCCA43300116.431621653108397RNA PCR Primer, Index 1 (100% over 28bp)
AGGTTCCGGATAAGTAAGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCA37543614.247131777885972RNA PCR Primer, Index 1 (100% over 28bp)
GTCCCACTCCGTAGATCTGTTCTGGAATTCTCGGGTGCCAAGGAACTCCA29219511.088283142371512RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA2435099.240735603674754No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGTATGC1382785.247405384626185RNA PCR Primer, Index 8 (100% over 50bp)
TGATACGGATGTTATACGCAGCTGGAATTCTCGGGTGCCAAGGAACTCCA351161.332590053996537RNA PCR Primer, Index 1 (100% over 28bp)
TAGCATATCGAGCCTGAGAACATGGAATTCTCGGGTGCCAAGGAACTCCA340251.2911885347770864RNA PCR Primer, Index 1 (100% over 28bp)
CCTGGAACTTAGGACGTGAATCTGGAATTCTCGGGTGCCAAGGAACTCCA294021.11575386626057RNA PCR Primer, Index 1 (100% over 28bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACT217900.8268919374810496RNA PCR Primer, Index 8 (100% over 36bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA191850.7280367976399237No Hit
TCTTAACCCGGACCAGAAACTATGGAATTCTCGGGTGCCAAGGAANTCCA173510.6584397433333498RNA PCR Primer, Index 1 (96% over 28bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA170960.6487629446156964RNA PCR Primer, Index 1 (100% over 34bp)
AGGTTCCGGATAAGTAAGAGCCTGGAATTCTCGGGTGCCAAGGAANTCCA149250.5663773367097139RNA PCR Primer, Index 1 (96% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA127560.484067625264262RNA PCR Primer, Index 1 (100% over 28bp)
GTCCCACTCCGTAGATCTGTTCTGGAATTCTCGGGTGCCAAGGAANTCCA115210.4372015608866073RNA PCR Primer, Index 1 (96% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGNGCCA101620.3856299159560544No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGTATGG71120.26988781364686665RNA PCR Primer, Index 8 (97% over 49bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATGTCGTATGC70110.2660550423900706RNA PCR Primer, Index 8 (98% over 50bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGAATTCTCGGGTGCC68870.2613494618371725No Hit
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG63060.23930154005302887RNA PCR Primer, Index 1 (100% over 29bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC62750.23812514491480435RNA PCR Primer, Index 5 (100% over 35bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGNATGC54610.20723528547884404RNA PCR Primer, Index 8 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCGCGTATGC53260.20211227439302756RNA PCR Primer, Index 8 (98% over 50bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC46220.17539672028625108RNA PCR Primer, Index 1 (100% over 27bp)
GAATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCA45560.1728921370887408RNA PCR Primer, Index 1 (100% over 28bp)
TCATAAACCGAACCAAAAACTATATAATAAAATGGAAAAAACGAAATCCA45230.17163984548998565No Hit
AGGTTCAGGATAAGTAAGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCA45190.17148805256892444RNA PCR Primer, Index 1 (100% over 28bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTT42910.16283585606843431RNA PCR Primer, Index 8 (100% over 37bp)
CATCGGTCGAACTTATGTGAAATGGAATTCTCGGGTGCCAAGGAACTCCA40160.15240009274547478RNA PCR Primer, Index 1 (100% over 28bp)
TCATGAGTCCGTACCTTGATTGTGGAATTCTCGGGTGCCAAGGAACTCCA38920.14769451219257665RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGTAAGC37770.14333046571206629RNA PCR Primer, Index 8 (98% over 50bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC37100.14078793428429068RNA PCR Primer, Index 1 (100% over 26bp)
TGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGTATGCC36330.13786592055386201RNA PCR Primer, Index 8 (98% over 50bp)
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA30570.1160077399210449RNA PCR Primer, Index 1 (100% over 23bp)
CCCGTGAAAGTAGGACATCGTCAGACATGGAATTCTCGGGTGCCAAGGAA30410.11540056823679998RNA PCR Primer, Index 1 (100% over 23bp)
AATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAG30100.11422417309857545RNA PCR Primer, Index 1 (100% over 29bp)
GATGTAACGAGTTGGAATGCAATGGAATTCTCGGGTGCCAAGGAACTCCA28830.10940474785488141RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC28700.1089114208614324RNA PCR Primer, Index 1 (100% over 27bp)
TCTTAAACCGGACCAGAAACTATGGAATTCTCGGGTGCCAAGGAACTCCA28600.10853193855877934RNA PCR Primer, Index 1 (100% over 28bp)
GCTCTACACTCTACTCGTCAGCTGTTGGAATTCTCGGGTGCCAAGGAACT28200.10701400934816703RNA PCR Primer, Index 1 (100% over 25bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATATCGTATGC27920.10595145890073844RNA PCR Primer, Index 8 (98% over 50bp)
GTCCGACGATCGTCCCACTCCGTAGATCTGTTCTGGAATTCTCGGGTGCC26640.10109408542677907No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG252.8957256E-669.779661
TCAGTGC1000.069.779661
ACGCGAC208.055308E-569.7796551
GGCCCTA150.002253220269.7796551
GGGCCGC150.002253220269.7796551
GTCCCCA650.069.7796551
GCATCCG650.069.7796551
GGATCCG1050.069.7319952
TACGGCC252.9075018E-669.7319954
TAACGCG500.069.7319954
GTGTCGC353.8307917E-969.7319952
TTGCGGA353.8307917E-969.7319954
TTAACGC500.069.7319953
CCCCACG252.9075018E-669.73199514
TTTGCTC252.9075018E-669.7319953
TTACGAT252.9075018E-669.7319956
CCTTCAC150.002259334569.731992
TTTAGAC150.002259334569.7319913
GGTACAG208.0826154E-569.7319916
GCGAACG150.002259334569.7319916
Produced by FastQC (version 0.11.5)