FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005284696

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284696
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31550163
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTTTCTCTTGCGTCT1962240.6219429040667713No Hit
CTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACT1537260.48724312454423774No Hit
GCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTT1305900.4139122831156213No Hit
GTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCC1077880.3416400733016815No Hit
CCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTTT986270.3126037732356565No Hit
GTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGC958580.3038272734121849No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGT954960.3026798942369965No Hit
GGCAATGCTTGTGGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTA913060.2893994557175505No Hit
GTTCAATCGTTTCTTTGGAAGGCAGTGGATTTTTCTCTTGCGTCTCTGTC770670.24426815164156201No Hit
GTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGT765800.24272457799980304No Hit
GCCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGG731030.23170403271767565No Hit
GGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGAC677860.21485150488762925No Hit
GTTTCTTTGGAAGGCAGTGGATTTTTCTCTTGCGTCTCTGTCTTCTTCAG652020.20666137287468211No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGC651610.206531421089647No Hit
GTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATT630090.1997105371531678No Hit
CGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTTTCTCTTGCGTCTCTGTCTTCTTC577240.18295943510656348No Hit
GGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGC559600.1773683387943194No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA540840.17142225224002805No Hit
CTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTG505170.16011644694196983No Hit
ATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGC454510.14405947760079718No Hit
GTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGT450530.14279799441923646No Hit
CTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGAA438130.13886774531085624No Hit
AGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGAC433710.13746680167706266No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTC424170.13444304550819594No Hit
CTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTTTC417010.13217364360367964No Hit
CTCCACTTTTTCAATTCTCTCTCCATTCTTCAGTAAGTCAACTTCAATGTCGGATGGATGAAACCCAGACACAT411770.13051279639981575No Hit
GGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTG407990.1293147043329063No Hit
GCTAAAAGAAGTAAAATAAGAAGGCAATGCTTGTGGAATGTACAGTGCAT404190.12811027315453172No Hit
GGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATC399170.12651915617678425No Hit
CATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGAAGG375590.11904534375939675No Hit
GTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGA367570.11650336006187986No Hit
GCTTGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTTTCTC362380.1148583606366788No Hit
GGTGAATTCAGTGTAGTACAAGAGATAGAAAGACCAGTCCTTGCTGAAAGACAAGTCTGAATGCTCCACTTTTTC361920.11471256107298082No Hit
GCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG358740.11370464235002525No Hit
CTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTA354890.11248436339298785No Hit
GTGGATTTTTCTCTTGCGTCTCTGTCTTCTTCAGTTTCGACTTATCGAAT354380.11232271605062707No Hit
CCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA344390.10915632987379495No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGC339290.10753985645018697No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTCTAATACTGGTG336280.106585820174685No Hit
GGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCT333430.10568249679090405No Hit
GTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCA330340.10470310406954157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGCG6500.055.05150225
CGCAACG3000.049.8033634
CGACGGA7300.048.54301518
TTACGCG6450.047.4263443
CGCGCAA6550.046.6803633
TCGCGCG7900.044.41665311
ACGCGAA1850.043.21325341
GCGCTAG9750.043.11456723
CGCATAC16450.041.690570
GTACGGT15450.040.8585560
AGCGCTA10850.038.4231922
ACCGTAT9200.037.04078747
CGCTAGA4850.036.5539861
CGCTAGG11500.036.25172824
TCGCGCC8600.034.8962161
AACTACG13000.033.91420465
ACTACGA12750.033.7969366
GACGCTA6650.033.4445637
CGCGACA9400.033.30617550
TTATGCT342900.033.01512570
Produced by FastQC (version 0.11.5)