FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005284704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32535553
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA2097900.6448023182516676No Hit
GTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGT1706820.5246015028544313No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCA1082650.3327590589900224No Hit
GTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAG1037900.31900487445226455No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG966950.2971979606432385No Hit
CAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAG956930.294118252731097No Hit
CCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACT956580.29401067810342735No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACAC950330.2920897026093271No Hit
GGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACC944170.29019638916234186No Hit
GGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGT943320.28993513649514424No Hit
CACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCA812320.24967149013880294No Hit
GTTTACCAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCT799810.24582646558981186No Hit
GCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGG770510.2368209324734699No Hit
AATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATG748210.22996689191052017No Hit
CCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCACGAGGGTTC747440.22973022772964702No Hit
CTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAAAAATCCACTGCCTTCCAA745450.2291185891323255No Hit
CCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTC694760.21353870948497478No Hit
TGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAA625110.19213135857872157No Hit
CCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTG560740.1723468477698842No Hit
ACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTT523330.16084865685239774No Hit
GGCAAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACC483640.1486496940746635No Hit
CTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCAAGACGAGAAGACCCTATGG464920.1428959882747344No Hit
ATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGC397070.12204187831078206No Hit
CTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAA386770.11887611069650483No Hit
CAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATA386630.11883308084543699No Hit
TGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAAT373350.11475139211557277No Hit
GGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCG368320.11320539103792089No Hit
CACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGC367610.1129871682217911No Hit
CGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGG356760.10965235476403305No Hit
AACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTTACCA353500.10865037394631036No Hit
GTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATA352660.1083921948399033No Hit
CCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCAT341740.10503586645661132No Hit
TATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAA340590.10468240696569688No Hit
CTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGC337420.10370808819508923No Hit
AATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCAAGACGAGAAGAC327660.10070829286350227No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)