FastQCFastQC Report
Thu 12 May 2022
EGAF00005284762

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284762
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57401883
Sequences flagged as poor quality0
Sequence length91
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT864507215.060606983920719No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA8646301.5062746286563458No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4783930.8334099423184428Clontech SMART CDS Primer II A (100% over 26bp)
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC4390330.7648407631505747No Hit
CTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCG4074330.7097903042658026No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA3938790.6861778384517456No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT3717280.6475885120353978No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG3330430.5801952524797837No Hit
TCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGG2444010.42577174689548075No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG2298030.4003405254144711No Hit
CGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTC1633520.2845760303716866No Hit
GCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGG1414350.2463943560875869No Hit
GTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTTG1365480.23788069809486911No Hit
ACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAG1188710.20708554107885277No Hit
CTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGCTCG1138070.1982635308322551No Hit
TAGCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGC951060.16568446021187144No Hit
TATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGC860480.14990449006698964No Hit
GGTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTT812930.14162078968733482No Hit
GTTGATACCAACTTAAATAATTATTAATGTTCTGACTTGCCCGACAAGTG677350.11800135546076077No Hit
GTTCTGACTTGCCCGACAAGTGATGGTGACTCTGTCTCCCACAGATGCAG627310.109283871401919No Hit
CTGAAATCTGTCCCAGATCCACTGCCACTGAACCTCGATGGGACTCCAGT614410.1070365583651672No Hit
ATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCA613060.10680137444271645No Hit
CTGGTTTTTGTTGATACCAACTTAAATAATTATTAATGTTCTGACTTGCC608900.10607665954094224No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG607810.10588677029985236No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTC605980.10556796542719687No Hit
GGAGTTTAGGGGCTTGGCCTGGTCTTTGTTGATACCAATTAAGAAAATTA595540.10374920976024427No Hit
GCCTGGTCTTTGTTGATACCAATTAAGAAAATTAGAAACGGGCTGACTTG588490.1025210270541125No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT574750.10012737735450247Clontech SMART CDS Primer II A (100% over 24bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)