FastQCFastQC Report
Thu 12 May 2022
EGAF00005284764

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284764
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67120998
Sequences flagged as poor quality0
Sequence length91
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT1003810514.955238001675719No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT11455221.7066522163451743No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG7013101.0448444166458908No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4793530.714162503960385Clontech SMART CDS Primer II A (100% over 26bp)
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA4566240.6802997774258362No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA3715530.5535570254780776No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC2601530.3875880987347655No Hit
CTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCG2431950.36232327773195505No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC2410360.3591066986220914No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT2085960.3107760704034824No Hit
CGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTC2020060.30095798039236543No Hit
GAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGA1602120.2386913257755792No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG1481880.2207774085838235No Hit
TCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGG1469960.21900151127073525No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1203170.17925389011647294No Hit
TATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGC1018610.15175727869838884No Hit
CTGGTTTTTGTTGATACCAATTTAAATAGTTGCCAATGTCCTGACTCGCC896370.13354539215879954No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC842730.12555385424990254No Hit
GCCAAATCGGATGCATCGGAGATCAGGAGTTTAGGGGGTTTTCCTGGTTT839920.12513520731619632No Hit
CTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGCTCG822240.12250115828134737No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT819150.12204079563894446No Hit
GTCCCAGATCCACTTCCACTGAACCTTGATGGGACCCCTGTTGCCAAATC803070.11964512208236236No Hit
CCCAGATCCACTTCCACTGAACCTTGATGGGACCCCTGTTGCCAAATCGG788730.11750868185839548No Hit
GGTGAAAGTAAAGTGTGTCCCAGATCCACTTCCACTGAACCTTGATGGGA772990.1151636630909451No Hit
GGGGGTTTTCCTGGTTTTTGTTGATACCAATTTAAATAGTTGCCAATGTC756800.11275160122023214No Hit
ATCGGAGATCAGGAGTTTAGGGGGTTTTCCTGGTTTTTGTTGATACCAAT740050.11025610793212581No Hit
GTTGATACCAATTTAAATAGTTGCCAATGTCCTGACTCGCCTGGCAAGTG737090.10981511329733208No Hit
ATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCA722410.1076280182842335No Hit
GGAGTTTAGGGGGTTTTCCTGGTTTTTGTTGATACCAATTTAAATAGTTG717800.10694119893747706No Hit
TAGCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGC696850.10381997001892015No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)