FastQCFastQC Report
Thu 16 Jun 2022
EGAF00005284771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences316250868
Sequences flagged as poor quality0
Sequence length16
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCTCGGTGGTCGC42899991.3565176997395625Illumina Single End PCR Primer 1 (100% over 16bp)
TCTACAGGAGACGCCT4815820.1522784753273784No Hit
GGACATTTGTGCGCCA4546730.14376972397748486No Hit
CAGATTTCTGCAGTTT4248900.13435220041830842No Hit
TCACTATTGAGACAAT4170930.1318867526396797No Hit
AACTCACGAATAGCAA4058230.12832312605700105No Hit
ATTCCAGGAGGACCAA4055220.12822794845261895No Hit
TTCCGGACTTTTCTTG4012310.126871114232009No Hit
GTCTAATCTTCAGGTT3927750.12419728757866998No Hit
GGTTCATGAGACACCC3780820.11955129242522743No Hit
GCCAGTCGACCTAGGC3734370.11808252175295214No Hit
TATCATGCTAATCGAA3695980.11686861204124822No Hit
AGAGAATACCTTTAGG3657030.1156369948682639No Hit
TCAAATCCTGGCATTT3641540.11514719384106165No Hit
GGGATGTGAACAGATA3629270.11475921071622167No Hit
TAAGGCTTGAGCCATT3606740.11404680160435166No Hit
TCTTCCTTGACCTTGA3555470.11242562028319872No Hit
GGTATCTGATGGAACG3508210.11093123703300002No Hit
CCTCCCTTGGAGAGGG3508170.11092997221433713No Hit
CCAACTCACTAAGCAA3480180.11004491535498331No Hit
GTTGATGACTAACCCA3437100.1086827056550561No Hit
CGCAATCCTTAACGAA3366050.1064360715051065No Hit
CTATGCCACGGAGTCA3365710.106425320546472No Hit
TATTGCTTGCCGATTA3349860.10592413615130379No Hit
CTAAGTCGATAATCGC3347850.10586057901349381No Hit
TACCGACCTACCGTTA3317280.1048939413503839No Hit
AGTGAAATGCAATAGA3310760.10468777590833364No Hit
TCCTAATTGCTCACGG3256720.10297900589477593No Hit
GTATCAGTGATCATCG3244750.10260050890990756No Hit
CTTTATGTGTGAGTAA3228140.10207529296014453No Hit
GGTATACTGCCTGAGC3207640.10142707339541594No Hit
CAAGTGGGATTGGGAG3165520.10009521934339798No Hit
AACTGTGACTGCATGT3165080.10008130633810625No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)