FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005284772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences340462400
Sequences flagged as poor quality0
Sequence length8
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCATCT11725410734.43966411562628RNA PCR Primer, Index 14 (100% over 8bp)
ACTGGAGC10667672531.332894616263058No Hit
GAAATCAA8797395325.839550270455707No Hit
CGGTCGTG166435964.888526897536996No Hit
NTCCATCT14707040.43197251737636816No Hit
NCTGGAGC13454070.3951705092838446No Hit
NAAATCAA11110310.326330014709407No Hit
TTCCATCA6401610.18802693043343408No Hit
ACTGGAGA3677010.10800047229885003No Hit
GAAATAAA3407240.1000768366785877No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)