FastQCFastQC Report
Thu 16 Jun 2022
EGAF00005284779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences340462400
Sequences flagged as poor quality0
Sequence length16
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCTCGGTGGTCGC25203740.7402796902095503Illumina Single End PCR Primer 1 (100% over 16bp)
CGTGCAGACGGAATAT6259200.18384408968508711No Hit
GGTAGTTTGCGAGTTT5628260.16531223418503777No Hit
TGGTCAAACGAGCTGA5585370.16405247686675534No Hit
CGACCTTACTGCAAGG5459390.16035221510510414No Hit
GATACGTACTGCACCG5456200.16025851900239202No Hit
TTTCTCGCTACCGTTA5291200.15541216886211223No Hit
TGGATTGACTAGACAA5050240.1483347353481618No Hit
ATGGCCGTGAATCTGC4971090.1460099558717791No Hit
TACCCGGGATCTGTAG4602310.13517821644915856No Hit
GGACGCTTGGCTGGCT4486410.1317740226233499No Hit
GCGTTAATGTGCTCAA4403250.12933146215264887No Hit
ATGTACGTGGCAGGAT4314190.12671560794965905No Hit
CCTAGCACTTCGTAAC4126580.12120516098106575No Hit
GGGTATACTTACCAAC4090890.1201568807598137No Hit
AGGAGGTTGTTGACAC4069050.11951540023215484No Hit
CTTGACGCTCCACATA3959210.1162891996296801No Hit
GGGTAGTGACCAACGC3906820.11475041003059369No Hit
GTCACAAACCCTGAGC3832090.11255545399433242No Hit
AGACTACTGATGTCAC3811100.11193893951285075No Hit
TCCTAATTGCTTCACC3702240.10874152329302736No Hit
TTTCGCTCTCTGTACA3697550.10860376946176728No Hit
GTTGTGATGCGCCACA3680800.10811179149298131No Hit
CCTACGGGACTTCAAG3643410.10701357917937487No Hit
TGGTCAAACAAGGTGC3630990.10664878118699746No Hit
GTTTCCCGAGGACAGT3624110.1064467030720573No Hit
CAATGCCCTACCGTTA3618270.10627517164891041No Hit
TGCTTCTCTAGCAATA3551400.10431107811024065No Hit
AACCCTCACAATACAG3530880.10370836838370405No Hit
TCGTGAATGATCTGTG3521350.1034284549483291No Hit
AATGGCTACGAACGTG3497750.10273528001917392No Hit
GGTTCATGATACCTGA3483110.10230527658854546No Hit
AGGTCGCACTCTGTGA3405810.10003483497737194No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)