FastQCFastQC Report
Tue 28 Jun 2022
EGAF00005284814

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284814
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences351064530
Sequences flagged as poor quality0
Sequence length16
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAACTTGCGTGCAC52596651.4982046178233956No Hit
TCTTGAACTGAGCTGA39719741.1314085191118566No Hit
TGGATCCCTTGCTGGC39221561.117217965597379No Hit
TGAGGCCACGCACCCA39202391.1166719121410529No Hit
TTCACGCTGGAAGGTC38049971.0838454685239776No Hit
CATAGTCGAACGAGCA35604871.0141973044101038No Hit
CATCGAAACCTTCACC34751380.989885819567132No Hit
AGATCTCGGTGGTCGC32925120.9378651839307149Illumina Single End PCR Primer 1 (100% over 16bp)
GCAGATACTTGTTCCC31595690.8999966473400204No Hit
AGTAAGTGATTGTGAC31027340.883807315994014No Hit
TCCCAGACTAGGCATG30425300.8666583320166238No Hit
TGGAAGTCTAGCTTGT30346720.8644199970871452No Hit
ACCTCCATGTTTGGGT30303830.8631982843723915No Hit
AGACGCAGAACGCCAC29499630.8402908149108654No Hit
CAGAGTACTATGAATG28188400.8029407015285764No Hit
CCGTCTATGTTGTACG26981780.7685703822029528No Hit
AAACGAAACCAACGCC25320130.7212386281234393No Hit
GGCAACGCTGACACCC25213220.7181933190459315No Hit
ATAGGAAACGGAATAT24703140.7036637965105732No Hit
TACCACCTGCGAATCT22986720.6547719303912588No Hit
AATCACCGAAACGAAT21704210.6182399002257505No Hit
ACTTCATACGACAAAT19391700.5523685346394863No Hit
CTCTGGACTCGAACGA19086360.5436709883507741No Hit
TACTGTACTTAACGGG18214310.5188308257743954No Hit
AATCGGACTTTTGGGT16320580.46488832124396046No Hit
TAATAGGGATTGTACG15672350.44642362473930364No Hit
ATACAGGCTTATTGCG15407930.43889167612575386No Hit
AACTACGACATAGGTA15242020.4341657643396785No Hit
CCCTGATTGGCTAATC14583870.41541849870164893No Hit
CCTTCTTTGCGAGGAT14561070.41476904545155846No Hit
GGTACCTCTAAATGCA14139100.40274931791029983No Hit
GAGGTTGGAACGTGCC14090500.4013649570351069No Hit
GTACTGCCTTGTGCTA14054630.40034320755788116No Hit
AGAATGTGATGACCTA12527010.3568292701059831No Hit
GCATATCGAACAGGCA11647210.33176835039415686No Hit
GACTATTCTTAGCGAG11430250.32558828999329553No Hit
TACATTGCTTTTCCGC11228310.31983607116332713No Hit
GAAGTAATGTCGACAT11159600.3178788811276377No Hit
GTTGATGACGCCACGT11058430.3149970747543194No Hit
TCGACCTTGCTACTCC10580210.3013750776815875No Hit
CATTCTATGCATCCTC8729150.24864801921173862No Hit
GTACATCGACAGCAAG8502620.2421953593545893No Hit
CATGTTACTCCTCGTT8318710.2369567213184425No Hit
GAGACTTACCGAGGAT7878430.22441543724169458No Hit
AACACTCGACGCACTG7758420.22099697739330146No Hit
TGTTTGTTGTCTGGGC7489560.21333855630473408No Hit
AGACTACTGTTCTGAG7396610.21069089491894838No Hit
ACCTCCAACAATCCTG6834100.1946679147562985No Hit
CGTGTCTACAGGCATG6615200.18843259385959613No Hit
GGAAGGACTAAGTAAC6540950.18631759807805134No Hit
TCGCGGTGAACCGTGC6438440.18339762208389437No Hit
GTTCCCAACATTGGTC6303560.17955559338335889No Hit
GCTGACCGACCTAGGC6152250.17524555955567483No Hit
ACTGGAGGAAATCTGC6142610.1749709661639699No Hit
TGCGAGGCTAAAGTAC6139940.17489491177020933No Hit
CTCTCTTTGCGACTAT6063120.17270671007407098No Hit
TTAGCCAGACCACATA6057610.1725497588719658No Hit
CCTAACGCTCCTATCC5935260.16906464461106338No Hit
TTCTACATGTGAGCAG5887890.1677153200296253No Hit
ACCGTTGCTTATGACC5851870.166689297833649No Hit
CGTGTCTACTCTGTAG5763440.1641703877062146No Hit
CTTTCGGTGACCTAGT5705190.16251114859139998No Hit
TACGTGAACCCATGTA5567690.15859448973668744No Hit
TCTTGGCACTTGCGCG5554500.15821877533455173No Hit
CAACAACACGATCCAT5286510.1505851360147378No Hit
CGTGTCTACTTACGAC5216530.14859177029362666No Hit
GTGTGATACAGGGCTA5159650.1469715553434008No Hit
CAATCAGACCACTCAA5146640.14660096820376584No Hit
AGCTTGTGATGAACAA5134560.14625687192038456No Hit
GTGCATAACAATGCGG5089230.14496565631395458No Hit
TGAAAGAACGCCCTGA5082260.14476711731601025No Hit
TATCAGCGACGTGCAC4985260.1420040925239585No Hit
CGGAATTTGATCACAC4977860.1417933050655958No Hit
TTCACGAGAAAGCTAA4964600.14141559672804313No Hit
CTCATAACTCGATCGC4880760.13902743179437696No Hit
TACCGCTACGTTAGGG4854080.13826745755260436No Hit
CGTGGGTACGCTTTGA4791560.13648658837735617No Hit
CAGTGGAGAATCTGTG4789050.13641509155026285No Hit
ATGACAAGAGCACTTA4763930.13569955358349647No Hit
CGTGGGTACTTGGCGC4757440.13551468728555402No Hit
AGGTAGCGATAACCCA4689720.1335856971936185No Hit
GACCAGGCTTCCGTAA4575990.130346121836917No Hit
AGCAGAGACAAGGCCA4572970.13026009776607167No Hit
AAACGAAACCCACATA4567110.13009317688688174No Hit
CTATGTTACTGTCACA4555770.12977015934933672No Hit
AGCGCAAGACCGGACT4555770.12977015934933672No Hit
GAGAGCCCTCGATTAC4501580.1282265684887049No Hit
AAGTTTCCTTAAAGCA4451390.12679691679475566No Hit
CACATATCTCGCTCGA4442000.12652944460096838No Hit
ATAGAACGACTCCTAC4364330.12431703083191001No Hit
AATTCTGCTCGCTCGA4328540.12329756013801793No Hit
GCTGACCGAGAGCTGA4272280.1216950057586279No Hit
GCATCCTTGCGACACC4251370.1210993887647949No Hit
TTCCAAACTGAATAAC4180980.11909434427909878No Hit
CGCGTTGGAGCGCTGT4179810.11906101707284412No Hit
CTGATTACTTCGTCAA4165780.11866137544570508No Hit
TAGTACCGATGCACCG4104940.11692836072046356No Hit
AGATACGCTATTAAGG4097710.11672241567668486No Hit
CCTCTTGGAAGTTGAA4096390.1166848157516796No Hit
CAAAGACCTCGTTCTC4086410.11640053753080666No Hit
AGGAATTGAAGGCATG4075180.11608065332034542No Hit
TGCAGTATGAGGGAGG4064780.11578441148697079No Hit
GGTATACTGGAGCTGA4029800.11478801347433192No Hit
CCCAGAGACCCGTTAA4027710.11472848025974028No Hit
GAGGTCCTGTGCGCCA3995870.1138215244929472No Hit
TTTGGGAACGAAGGTC3975680.11324641654911706No Hit
GCGTTTAGAGCTAATC3958580.11275932661154917No Hit
TCGTCCAACCGTGCAC3955050.11265877529695181No Hit
AAATGCGCTCGAATCT3947700.11244941207817263No Hit
TGCGCCAGACAGGCCT3931050.11197514029685654No Hit
CTATTGGGAAATCTGC3928750.11190962527601407No Hit
CAAAGACCTTGCTGGC3906750.11128295985926007No Hit
CGCACTTACCCTTCCA3897620.11102289371130716No Hit
TTCGATGGACGAGGTA3885340.11067310046959174No Hit
CGCAAAGGACCAAACT3849860.10966246006111754No Hit
TTCCCGAACCAGGCCT3810520.10854186835679469No Hit
GTCCGAGTGTGAGCAG3779530.10765912466292166No Hit
TGACTCAGACCAAACT3759150.10707860460867408No Hit
TCAATCGGACCGTGAC3754830.10695555030865694No Hit
ACTGCTAGAATTGGTC3744980.10667497511070115No Hit
TAATGGGCTGGGTAGT3732130.10630894553773347No Hit
TTCTTAGACTAAGCAA3702440.10546323207303228No Hit
TGTCTGCACAGCTGAG3692380.10517667506882565No Hit
ACTACGCCTTCTGTAG3645110.10383019896655467No Hit
ACTCTAACTGTTGACT3641450.10372594462904014No Hit
AGGACGCCTTCTGGAT3637900.10362482361860938No Hit
AACCGCTCTCTTAAGC3628380.1033536484019049No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)