FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005284834

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284834
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79150878
Sequences flagged as poor quality0
Sequence length91
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA49176996.213069424195143No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT27508903.475501560450157Clontech SMART CDS Primer II A (100% over 26bp)
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG26345363.328498769148208No Hit
GCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGG22305302.818073603681314No Hit
ACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAG6602590.8341777333158579No Hit
CTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGCTCG4184150.5286296381955485No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2785030.3518634373203036No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2426760.30659925212705785Clontech SMART CDS Primer II A (100% over 24bp)
GTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTTG2147010.27125536118500165No Hit
CTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGGT1991550.2516143914411158No Hit
TAGCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGC1878810.23737070863572735No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGCGTGGTCATCTC1369300.1729987126611533No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT1357740.17153821085850746No Hit
CTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGGTTGG1237150.15630275130997284No Hit
AGCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGCT1210990.1529976711060615No Hit
GCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGCTC1184570.1496597422456893No Hit
GGTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTT1049330.13257338724656978No Hit
GCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGGTTG1030220.1301590109966942No Hit
ATGACGTCCTTGGAAGGCAGCAGCACCTGTGAGGTGGCTGCGTACTTGCC1011840.12783686366688188No Hit
CTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGCTCGGGC962030.12154381913489322No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT918590.11605556668619645Clontech SMART CDS Primer II A (100% over 25bp)
CTGCTGGCCGCGGTACTGTTGGTCATGGAGCTGTTGATATCTTCCTCCAG814160.10286177747769268No Hit
GGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTTGGG811270.1024966520270312No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)