FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005284845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66290711
Sequences flagged as poor quality0
Sequence length91
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC27144714.094798440161548No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC13030081.9655966580295088No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC7248041.0933718903693763No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC4359910.6576954650554283No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT3354020.5059562568275969No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGTTGG2884190.4350820735653295No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG2774720.41856844769699336No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC1924470.2903076420465606No Hit
CTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATGACC1287400.19420518811451576No Hit
CTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGTTGGACC1248510.18833860448411846No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC1173170.1769735129255138No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATGA955230.14409711188646024No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG930820.14041484635758394No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGTTGGA930680.1403937272599173No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT879690.13270185018833183Clontech SMART CDS Primer II A (100% over 26bp)
CAGGCAGTATCTGGAGTCATTGAGGGCGGGCTGCTCCTTGAGGGGCTGCG826080.12461474428898493No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)