FastQCFastQC Report
Tue 28 Jun 2022
EGAF00005284881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences355347386
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCTCGGTGGTCGC22392050.6301453417754985Illumina Single End PCR Primer 1 (100% over 16bp)
AATTGCCGAAATGCGG5201810.1463866122262681No Hit
GTTGTTGGAGAATGTA4995450.14057933720103402No Hit
GATCTGCCTGCCCTTT4809820.13535543497708466No Hit
ATTGAGAGATGAGGCA4726400.13300787303385425No Hit
GCGTAAGGAAGCCTAT4366500.12287975575540043No Hit
ATGCGCGTGAGACAAT4240460.11933280409722784No Hit
AAATAGGACATCCGTA4043090.11377852094288377No Hit
CGATCGGTGGGAATAT3858760.10859120263797296No Hit
TAGTGAAGAGCCCTTT3830530.1077967687653118No Hit
ATCATCTACCATCCTC3750530.1055454506706291No Hit
CTAATCTACCCGCAGT3681070.10359074373492085No Hit
TAGACCTGACATACTG3646070.10260579206849715No Hit
TATTGGTCTGCTGAGA3570300.10047351241807082No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)