FastQCFastQC Report
Thu 16 Jun 2022
EGAF00005284887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61853112
Sequences flagged as poor quality0
Sequence length91
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC30624164.951110624797666No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC14546372.351760409403491No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGTTGG11303311.8274440257751299No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC8680351.4033812882365564No Hit
CTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGTTGGACC4975460.8043993000707871No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG4938100.798359183609064No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT3900820.630658648185721No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC3750520.6063591432553951No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGTTGGA3630130.58689528830821No Hit
CTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATGACC2292080.3705682585542341No Hit
CTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGTTGGACCAA1938100.3133391251195251No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC1719860.27805553259793947No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATGA1674760.27076406438531336No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC1056010.170728677321846No Hit
CTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATGACCGT894690.14464753204333516No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT848180.13712810440321901Clontech SMART CDS Primer II A (100% over 26bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG781700.12638005990709086No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGCTTT771890.12479404431583005No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGTTG759520.12279414494132486No Hit
TCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGTTGGACCA727200.11756886217786423No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATATGGGTTT719440.11631427695990462No Hit
CAGGCAGTATCTGGAGTCATTGAGGGCGGGCTGCTCCTTGAGGGGCTGCG696920.11267339305417648No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)