FastQCFastQC Report
Thu 12 May 2022
EGAF00005284923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69117043
Sequences flagged as poor quality0
Sequence length91
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT62084218.982474843433334No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA7174111.0379654118015436No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4150790.6005450782956673Clontech SMART CDS Primer II A (100% over 26bp)
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG3830270.55417156662793No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT3220660.46597190218337325No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA3057470.4423612277510194No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG2794680.4043402146124799No Hit
GCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGG1942750.28108117993415893No Hit
GTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTTG1462370.21157878527876256No Hit
CGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTC1409020.2038599944155597No Hit
CTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGCTCG1172590.16965280184223158No Hit
ACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAG992950.14366210660950873No Hit
TATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGC740940.10720076667631745No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG732070.10591743631161998No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT698120.10100547848958179Clontech SMART CDS Primer II A (100% over 24bp)
GAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGA697370.10089696690293883No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)